Development of technology for biosynthesis new polysaccharide by the bacteria

几种糖被鉴定为木醋杆菌的碳源。主要检测甘油、甘油醛等三碳糖。在SH培养基中用甘油代替葡萄糖作碳源，提高了产量，实现了长时间培养。通过碳13核磁共振分析，明确了以碳13标记的甘油为碳源的多糖代谢途径。结果表明，甘油衍生物被消化后重新与葡萄糖分子结合，并改变了传统含葡萄糖酶的SH培养基的组成。结果表明，氨基水解物可以有效地替代细菌蛋白胨和耶斯特提取物，从而消除细菌纤维素的产生。在培养基中加入硝酸铵和硝酸钠作为培养基的氮源。在前一个实验中， 关于我们 h作为氯化铵有效地将N-乙酰葡糖胺引入纤维素主链中。硝酸盐的使用对于在纤维素骨架中引入N-乙酰葡糖胺也是有效的。然而，酶消化和分子量测定表明，硝酸盐诱导的多糖结构不同于铵盐诱导的多糖结构。为了提高细菌纤维素的产量，我们设计了一种新型的浅盘培养器，用于细菌纤维素的生物合成。这是基于木醋杆菌是一种产碳细菌这一事实而提出的一种全新的思路。利用该技术，将细菌纤维素制成纤维状、薄膜状等多种形态。该膜可制成长条形和非长条形。由于生物合成的细菌纤维素薄膜被缓慢地去除，因此细菌纤维素在新形成的培养基表面连续地形成。因此，培养基有效地用于细菌纤维素的生物合成。少

Several sugars were elucidated for the carbon source of Acetobacter xylinum. Three carbon sugars such as glycerol and glyceraldehyde were mainly examined. Improvement of yield and long tena cultivation were aacomplished by the use of glycerol as a carbon source instead of glucuse in the SH medium. Carbon thirteen NMR examination of the obtained polysacaharide was carried out to clarify the biosynthesis path way of the metabolism using carbon thirteen labeled glycerol as a carbon source. As a result, it was found that glycerol derivatives was digested and recunstnictedto glucese molecule again.In addition, medium cumposition was changed from the cunventional SH medium cuntaining glucase. It was found that amino hydrolysate was effective instead for bactopeptone and yeste extract, which lead the elimination of production cast of bacterial cellulose. Ammonium nitrate and sodium nitrate were added in the medium as a nitrogen source of the medium. In the former experiment, ammonium salt suc … More h as ammonium chloride was effective for the introduction of N-acetylglucosamine in the cellulose backbone. The use of nitrate was also effective for the introduction of N-acetylglucusamineinthe cellulose backbone. However enzyme digestive and molecular weight measurements revealed that the structure of the nitrate induced polysacaharide was different from that induced from ammonium salts. It was found that N-acetylglucusamine residue existed in the side chain of the cellulose backbone not in the cellulose backbone.In order to improve the yield of the bacterial cellulose, shallow pan cultivator was newly designedfor the biosynthesis of bacterial cellulose. This is a completely new ideabased on the fact thatAcetobacter xylinum is an arcobic bacteria Using this tethnology, bacterial cellulose is fabricated in several shapes, such as fiber, thin film. The film can be fabricated into elongated one and non-elongated one. Since bio synthesized bacterial cellulose thin film was removed slowly bacterial cellulose was continuously formed in the newly formed surface of the medium. Therefore, medium was effectively used for the bio synthesis of bacterial cellulose. Less