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Development of technology for biosynthesis new polysaccharide by the bacteria

细菌生物合成新型多糖技术开发

基本信息

批准号：
14593007
负责人：
TAMURA Hiroshi
金额：
$ 2.11万
依托单位：
Kansai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14593007/
关键词：
Acetobacter xvlinum Bacterial cellulose Cultibation Elongation Degree of crvstalinitv Film

项目摘要

Several sugars were elucidated for the carbon source of Acetobacter xylinum. Three carbon sugars such as glycerol and glyceraldehyde were mainly examined. Improvement of yield and long tena cultivation were aacomplished by the use of glycerol as a carbon source instead of glucuse in the SH medium. Carbon thirteen NMR examination of the obtained polysacaharide was carried out to clarify the biosynthesis path way of the metabolism using carbon thirteen labeled glycerol as a carbon source. As a result, it was found that glycerol derivatives was digested and recunstnictedto glucese molecule again.In addition, medium cumposition was changed from the cunventional SH medium cuntaining glucase. It was found that amino hydrolysate was effective instead for bactopeptone and yeste extract, which lead the elimination of production cast of bacterial cellulose. Ammonium nitrate and sodium nitrate were added in the medium as a nitrogen source of the medium. In the former experiment, ammonium salt suc … More h as ammonium chloride was effective for the introduction of N-acetylglucosamine in the cellulose backbone. The use of nitrate was also effective for the introduction of N-acetylglucusamineinthe cellulose backbone. However enzyme digestive and molecular weight measurements revealed that the structure of the nitrate induced polysacaharide was different from that induced from ammonium salts. It was found that N-acetylglucusamine residue existed in the side chain of the cellulose backbone not in the cellulose backbone.In order to improve the yield of the bacterial cellulose, shallow pan cultivator was newly designedfor the biosynthesis of bacterial cellulose. This is a completely new ideabased on the fact thatAcetobacter xylinum is an arcobic bacteria Using this tethnology, bacterial cellulose is fabricated in several shapes, such as fiber, thin film. The film can be fabricated into elongated one and non-elongated one. Since bio synthesized bacterial cellulose thin film was removed slowly bacterial cellulose was continuously formed in the newly formed surface of the medium. Therefore, medium was effectively used for the bio synthesis of bacterial cellulose. Less

木醋酸菌的碳源有几种糖类。重点考察了甘油和甘油醛等三种碳糖。在SH培养基中用甘油代替葡萄糖作碳源，可提高产量，延长培养时间。以碳13标记的甘油为碳源，对得到的多糖进行了碳13核磁共振检测，以阐明其代谢的生物合成途径。结果发现，甘油衍生物被消化并重新结合成葡糖分子，此外，还改变了传统的含有葡萄糖酶的SH培养基。结果表明，氨基水解物能有效地替代细菌内酯和耶斯特提取液，从而消除细菌纤维素的产生型。在培养基中加入硝酸铵和硝酸钠作为氮源。在前一次实验中，铵盐加入…。氯化铵浓度越高，N-乙酰氨基葡萄糖在纤维素骨架中的引入越有效。硝酸盐的使用也能有效地将N-乙酰氨基葡萄糖胺引入纤维主干。然而，酶消化和相对分子质量测定表明，硝酸盐诱导的多糖与铵盐诱导的多糖结构不同。发现N-乙酰葡糖胺残基存在于纤维素骨架的侧链中，而不存在于纤维素骨架中，为了提高细菌纤维素的产量，新设计了用于细菌纤维素生物合成的浅盘培养机。这是一种全新的概念，基于木醋酸菌是一种弧菌，利用这种技术，细菌纤维素被制造成几种形状，如纤维、薄膜。该薄膜可制成拉长薄膜和非拉长薄膜。由于生物合成的细菌纤维素薄膜被缓慢去除，细菌纤维在新形成的介质表面不断形成。因此，培养基能有效地用于细菌纤维素的生物合成。较少