In vivo patch-clamp analysis of the thermal response in VR-1 knockout mouse

VR-1 敲除小鼠热反应的体内膜片钳分析

基本信息

  • 批准号:
    15300135
  • 负责人:
  • 金额:
    $ 8.19万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

Recently a capsaicin receptor named VR-1 has been cloned and further clarified to respond to noxious heat and proton, and turned out to be one of the TRP family. However, the physiological significance of the receptor in in vivo has not been elucidated yet. To clarify the function of the receptor in in vivo condition, we need an in vivo patch-clamp recording method which enables us to evaluate receptor's function under the physiological conditions. Whole cell patch-clamp recordings were made from substantia gelatinosa (SG) neurons that received predominantly C afferent fibers in the mouse spinal cord in vivo. Unexpectedly, none of SG neurons tested responded to noxious heat, in spite of the densest termination of C afferents from the skin. Anatomical data has reported that a subpopulation of deep dorsal horn neurons extends their dendrites to the SG and receives inputs from C afferent fibers. Thus, we next made recordings from deep dorsal horn neurons and analyzed a heat response. As expected, about 20 % of neurons responded to noxious heat stimulation with increasing a barrage of fast EPSCs. However, no slow synaptic current was observed in all neurons tested, suggesting that the heat sensation is transmitted by a release of glutamate onto the dendrites of deep dorsal horn neurons. We further confirmed whether SG neurons received C afferent inputs expressing TRPV1 receptors by application of capsaicin during the recording from SG neurons in the spinal cord slices. Application of capsaicin elicited a barrage of mEPSCs, indicating that all SG neurons received C afferent inputs which were endowed the TRPV1 receptor. This clear discrepancy is required to be resolved.
最近,一个被命名为VR-1的辣椒素受体被克隆并进一步阐明了对有害热和质子的反应,并且被证明是TRP家族的一员。然而,该受体在体内的生理意义尚未阐明。为了阐明受体在体内的功能,我们需要一种在体膜片钳记录方法,使我们能够在生理条件下评估受体的功能。采用全细胞膜片钳技术记录了小鼠脊髓胶状质(SG)主要接受C类传入纤维的神经元。出乎意料的是,没有SG神经元测试响应有害的热,尽管从皮肤的C传入的denanderous终止。解剖学数据已经报道,深背角神经元的亚群将它们的树突延伸到SG并接收来自C传入纤维的输入。因此,我们接下来从深背角神经元进行记录并分析热反应。正如预期的那样,大约20%的神经元对有害的热刺激作出反应,增加了快速EPSC的弹幕。然而,在所有测试的神经元中没有观察到缓慢的突触电流,这表明热感觉是通过释放谷氨酸到深背角神经元的树突上来传递的。我们进一步证实了是否SG神经元接受C传入输入表达TRPV 1受体的辣椒素在记录过程中从SG神经元在脊髓切片。辣椒素的应用引起了mEPSC的弹幕,表明所有SG神经元接受C传入输入,赋予TRPV 1受体。这一明显的差异需要解决。

项目成果

期刊论文数量(166)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Brain-Inspired IT I
脑启发IT I
Yang K: "Pre-and postsynaptic inhibition mediated by GABA_B receptors in rat ventrolateral periaqueuctal gray neurons."Biochemical and Biophysical Research Communication. 302. 233-237 (2003)
Yang K:“大鼠腹外侧导水管周围灰色神经元中 GABA_B 受体介导的突触前和突触后抑制。”生物化学和生物物理研究通讯。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
In vivo patch-clamp analysis of effects of noradrenaline on spmatosensory input to substantia gelatinosa.
去甲肾上腺素对凝胶质的液体感觉输入的影响的体内膜片钳分析。
The role of dopamine receptors in spinal antinociception.
多巴胺受体在脊髓抗伤害作用中的作用。
The difference between excitatory and inhibitory projection fields of primary nociceptive afferent.
初级伤害性传入的兴奋性和抑制性投射场之间的差异。
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YOSHIMURA Megumu其他文献

YOSHIMURA Megumu的其他文献

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{{ truncateString('YOSHIMURA Megumu', 18)}}的其他基金

Clarification of dorsal root evoked synaptic responses in lamina I neurons
阐明 I 层神经元背根诱发的突触反应
  • 批准号:
    21600005
  • 财政年份:
    2009
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Clarification of nociceptive transmission in the primary somatosensory cortex
初级体感皮层伤害性传递的澄清
  • 批准号:
    17200027
  • 财政年份:
    2005
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
In vivo patch-clamp analysis of hyperalgesia induced in ovarlectomized rats
去卵巢大鼠痛觉过敏的体内膜片钳分析
  • 批准号:
    13480276
  • 财政年份:
    2001
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
In vivo patch clamp analysis of pain transmission in the spinal dorsal horn
脊髓背角疼痛传递的体内膜片钳分析
  • 批准号:
    10680766
  • 财政年份:
    1998
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Long lasting slow IPSP in substantia gelatinosa of the rat spinal cord
大鼠脊髓胶质质中持久缓慢的 IPSP
  • 批准号:
    07680905
  • 财政年份:
    1995
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

In vivo patch clamp analysis of diabetic cystopathy for treatment
糖尿病性膀胱病治疗的体内膜片钳分析
  • 批准号:
    19J11791
  • 财政年份:
    2019
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  • 项目类别:
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Elucidation of spinal mechanism of chronic myalgia using novel in vivo patch clamp method and search for therapeutic agents.
使用新型体内膜片钳方法阐明慢性肌痛的脊柱机制并寻找治疗药物。
  • 批准号:
    19K09323
  • 财政年份:
    2019
  • 资助金额:
    $ 8.19万
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Development of in vivo patch-clamp recording technique from the anterior cingulate cortex
前扣带皮层体内膜片钳记录技术的发展
  • 批准号:
    16K15341
  • 财政年份:
    2016
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Elucidation of mechanism of itch transmission to spinal cord using in vivo patch clamp and three-dimensional electron microscope.
使用体内膜片钳和三维电子显微镜阐明瘙痒向脊髓传播的机制。
  • 批准号:
    15K08667
  • 财政年份:
    2015
  • 资助金额:
    $ 8.19万
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    Grant-in-Aid for Scientific Research (C)
Investigation of sensory integration in the insular cortex -multiple in vivo patch clamp analysis-
岛叶皮质感觉统合研究-多重体内膜片钳分析-
  • 批准号:
    26462824
  • 财政年份:
    2014
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of the mechanisms of acupuncture analgesia using in vivo patch-clamp technique
体内膜片钳技术针刺镇痛机制分析
  • 批准号:
    26460711
  • 财政年份:
    2014
  • 资助金额:
    $ 8.19万
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    Grant-in-Aid for Scientific Research (C)
In vivo patch-clamp analysis of central cytokine action on visceral functions
体内膜片钳分析中枢细胞因子对内脏功能的作用
  • 批准号:
    26860391
  • 财政年份:
    2014
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    $ 8.19万
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In vivo patch-clamp analysis of spinal GABA-mediated inhibitory system
脊髓 GABA 介导的抑制系统的体内膜片钳分析
  • 批准号:
    25670289
  • 财政年份:
    2013
  • 资助金额:
    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
In vivo patch-clamp analysis of itch transmission in central nerve system
中枢神经系统瘙痒传播的体内膜片钳分析
  • 批准号:
    25860431
  • 财政年份:
    2013
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    $ 8.19万
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    Grant-in-Aid for Young Scientists (B)
Activated microglia induce neuropathic pain after spinal cord injury -in vivo patch-clamp analysis-
激活的小胶质细胞在脊髓损伤后诱发神经性疼痛-体内膜片钳分析-
  • 批准号:
    24791558
  • 财政年份:
    2012
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    $ 8.19万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
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