DEVELOPMENT OF CAPILLARY AND MICROCHIP SEPARATION-DETECTION SYSTEMS TOWARD PROTEOMICS
DEVELOPMENT OF CAPILLARY AND MICROCHIP SEPARATION-DETECTION SYSTEMS TOWARD PROTEOMICS
批准号:
15350041
负责人:
OTSUKA Koji
金额:
$9.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2004
中文摘要
针对毛细管电泳(CE)和微芯片电泳(MCE)对蛋白质的高效分离和检测,主要研究了以下五个方面。1)建立了一种高性能的微芯片等电聚焦(MCIEF)分析方法。MCIEF在210 s内成功分离了4个模型蛋白,其中蛋白的pI值与其聚焦位置呈良好的线性关系。2)开发了一种固定化蛋白质的高效手性分离技术——毛细管电色谱和微芯片电色谱。以亲和素为手性选择剂,成功地分离了4对对映体。3)在线预富集法提高MCE检测灵敏度。在MCE的胶束电动色谱(MEKC)模式下,利用新设计和制造的T -cross型通道石英芯片,成功地实现了SRW在线预富集。4)利用热透镜显微镜(TLM)提高CE和MCE的检出率。在微芯片MEKC-TLM系统中,以扫描作为在线样品预富集技术,获得了日落黄在4 nM或8 amol绝对注射量下的检出限。5)开发了一种具有质谱(MS)检测的高性能MCE系统。建立了一种具有电喷雾电离界面的MCE-MS系统,成功地分离和检测了部分肽和蛋白质。
英文摘要
Toward the high performance separation and detection of proteins by capillary electrophoresis(CE) and microchip electrophoresis(MCE), following five subjects were mainly investigated.1)Development of a high performance analysis method by microchip isoelectric focusing(MCIEF). Four model proteins were successfully separated within 210 s by MCIEF, where good linear relationship between a pI value of the protein and its focused position.2)Development of a highly efficient chiral separation technique by capillary electrochromatography and microchip electrochromatography using immobilized proteins. Avidin was used as a chiral selector and four pairs of enantiomers were successfully resolved.3)Improvement of detection sensitivity in MCE using on-line sample preconcentration. Under a micellar electrokinetic chromatography (MEKC) mode in MCE, successful on-line sample preconcentration by SRW was achieved by using a newly designed and fabricated T -cross type channel quartz chip.4)Improvement of detectability in CE and MCE by using thermal lens microscopy (TLM). In a microchip MEKC-TLM system with sweeping as an on-line sample preconcentration technique, the limit of detection of 4 nM or 8 amol in absolute amount injected for Sunset Yellow was obtained.5)Development of a high performance MCE system with mass spectrometric (MS) detection. An MCE-MS system with an electrospray ionization interface was developed, where some peptides and proteins were successfully separated and detected.
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Preparation of uniformy sized molecularly imprinted polymers for phenolic compounds and their application to the assay of bisphenol A in river water
均匀尺寸酚类分子印迹聚合物的制备及其在河水中双酚A测定中的应用
DOI:
--
发表时间:
2003
期刊:
Anal.Sci. 19
影响因子:
--
作者:
[H.Sanbem, K.Hosoya, J.Haginaka]
通讯作者:
J.Haginaka
分離技術(マイクロ化学チップの技術と応用)(化学とマイクロ・ナノシステム研究会 監修;北森武彦, 庄子習一, 馬場嘉信, 藤田博之 編)
分离技术(微量化学芯片的技术与应用)(化学与微纳系统研究组指导;北森武彦、翔子修一、马场庆信、藤田博之主编)
DOI:
--
发表时间:
2004
期刊:
影响因子:
--
作者:
[北川文彦, 大塚浩二]
通讯作者:
大塚浩二
Chiral micellar electrokinetic chromatography (Chapter 20) in Chiral separations - Methods and protocols (Guebitz, G.; Schmid, M.G., Eds.)
手性分离中的手性胶束电动色谱(第 20 章)- 方法和方案(Guebitz, G.;Schmid, M.G.,编辑)
DOI:
--
发表时间:
2003
期刊:
影响因子:
--
作者:
[K.Otsuka, S.Terabe]
通讯作者:
S.Terabe
A possible purification method of DNAs' fragments from humic matters in solid extracts using novel stimulus responsive polymer adsorbent
使用新型刺激响应聚合物吸附剂从固体提取物中的腐殖质中纯化 DNA 片段的可能方法
DOI:
--
发表时间:
2003
期刊:
J.Pharm.Biomed.Anal. 30
影响因子:
--
作者:
[K.Hosoya, T.Kubo, N.Tanaka, J.Haginaka]
通讯作者:
J.Haginaka
Retentivity and enentioselectivity of uniformly sized molecularly imprinted polymers for d-chlorpheniramine and -brompheniramine in hydro-organic mobile phase
均匀尺寸的分子印迹聚合物在水有机流动相中对 d-扑尔敏和 溴苯那敏的保留性和对映体选择性
DOI:
--
发表时间:
2004
期刊:
J.Chromatogr.B 804
影响因子:
--
作者:
[J.Haginaka, C.Kagawa]
通讯作者:
C.Kagawa
共 45 条
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Development of Seaweed Bed Restoration Technology Using Deep Ocean Water
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Viscous Hydroelasticity of Riser for Pumping-Up Deep Ocean Water
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Development of Ecosystem Model for Optimum Design of Artificial Lagoon
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DEVELOPMENT OF HIGH PERFORMANCE TECHNIQUES FOR CHIRAL SEPARATION AND DETECTION BY CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY
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High Performance Analysis of Environmental-Interest Analytes by Non-Aqueous Capillary Electrophoresis
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WATER PERMEABILITY OF REINFORCED CONCRETE MEMBER WITH NON-PENETRATION CRACK.
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SIZE EFFECT IN STRENGTH OF CONCRETE STRUCTURES AND PROPERTIES OF FRACTURE PROCESS ZONE
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DETECTION OF FRACTURE PROCESS ZONE IN CONCRETE BY X-RAY WITH CONTRAST MEDIUM
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