High Performance Analysis of Environmental-Interest Analytes by Non-Aqueous Capillary Electrophoresis

研究了非水溶液毛细管电泳（CE）分析环境样品或环境污染物的方法。研究了非水毛细管电泳手性分离、环糊精衍生物组成对水相毛细管电泳手性分离的影响、在线毛细管电泳-质谱立体选择性分离检测除草剂对映体、新型手性表面活性剂胶束电动色谱手性分离以及在线富集高灵敏度分析二恶英类化合物.发现二甲基甲酰胺、二甲基亚砜（DMSO）、丙酮和四氢呋喃（THF）是非水CE的有效溶剂。2.采用非水毛细管电泳法，以β-CD/DMSO或β-CD/甲酰胺为流动相，实现了除草剂对映体和丹磺酰-DL-氨基酸的手性分离.药物组分对映体的手性分离通过使用OD-R聚合物/丙酮或OD-R聚合物/THF溶液的非水CE实现。(OD-R聚合物是手性聚合物。）4.考察了市售二甲基-β-环糊精的组成对环糊精修饰毛细管区带电泳手性拆分的影响.成功地实现了β-环糊精衍生物对苯氧酸类除草剂对映体的CE-MS手性分离和检测.采用新型手性表面活性剂对部分药物组分对映体和DL-氨基酸衍生物进行了光学拆分.利用CD修饰的MEKC，采用在线富集技术对二恶英相关化合物进行了高灵敏度分析.采用毛细管电色谱手性固定相，实现了药物组分的手性分离。

Analysis of environmental-interest analytes or environmental pollutants by capillary electro phoresis (CE) using non-aqueous solutions was investigated. Chiral separations by non-aqueous CE, effects of compositions of cyclodextrin (CD) derivatives in chiral separations by aqueous CE, stereoselective separation and detection of herbicides enantiomers by on-line CE - mass spectrometry (MS), chiral separations by micellar electrokinetic chromatography (MEKC) using novel chiral surfactants, and high sensitive analysis of dioxins and related compounds by MEKC with on-line concentration were studied.1. Dimethylformamide, dimethylsulfoxide (DMSO), acetone, and tetrahydrofuran (THF) were found to be effective solvents for non-aqueous CE.2. Chiral separations of herbicide enantiomers and dansyl-DL-amino acids were achieved by non-aqueous CE using a beta-CD/DMSO or beta-CD/formamide solution.3. Chiral separations of drug component enantiomers were achieved by non-aqueous CE using an OD-R polymer/acetone or OD-R polymer/THF solution. (OD-R polymer is a chiral polymer.)4. Effects of compositions of commercial dimethyl-beta-CDs on chiral recognitions in CD modified capillary zone electrophoresis were investigated.5. Chiral separation and detection of phenoxy acid herbicide enantiomers by CE-MS using beta-CD derivatives was successfully achieved.6. Some drug component enantiomers and DL-amino acid derivatives were optically resolved by MEKC using novel chiral surfactants.7. High sensitive analysis of dioxin related compounds were carried out by CD modified MEKC using an on-line concentration technique.8. By using capillary electrochromatography with chiral stationary phases, chiral separations of some drug components were achieved.