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Development of chromatography System for multi components with high-yield, high-purity, high-concentration and high-productivity

高产率、高纯度、高浓度、高产率多组分色谱系统开发

基本信息

批准号：
15360409
负责人：
ISHIDA Masaru
金额：
$ 6.66万
依托单位：
TOKYO INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15360409/
关键词：
HPLC Sandwiching Recycle System 2ループリサイクルシステム

项目摘要

A new HPLC recycling system for multi-component separation is proposed. This system uses the interference of broadening by recycling the residual components on both sides of the introduced feed. By using this system, the high purity, high concentration, and high yield of target components are achieved. Furthermore, reduction of the eluent consumption is achieved. In this research simulations and experiments are performed.(1)Experiments with preparative columnThe separation of trans-stilben oxide that is used for calibration of separation column is performed. The size of the column, pump, valves, tubes are chosen for preparative separation. The influence of diffusion from those enlarged size of equipments is not observed, and the separation of 2 target components and the impure component is achieved. The proposed system is compared with the conventional simple recycle system, and the results showed the obvious advantage in yield, concentration and productivity.(2)Development of tube with variable volumeIn this system, the tube is used as volume for keeping the chromatography profile. The change in the condition of target components such as the concentration and required yield is handled by changing the length of the tubes. The tube with variable volume is developed and its availability is confirmed by the experiment.(3)Establishment of planning system with high-speed simulatorThe HPLC simulator is developed for planning the volume of tube and the timing of change of the valves. The chromatography of the target mixture with roughly separated is used for setting the parameters of the simulator. By using this simulator, the accurate prediction of result of separation can be obtained.

提出了一种用于多组分分离的高效液相色谱循环系统。该系统通过回收引入进料两侧的残留组分来利用加宽的干扰。通过使用该系统，实现了目标组分的高纯度、高浓度和高收率。此外，实现了洗脱剂消耗的减少。在这项研究中，模拟和实验进行。（1）进行了反式氧化芪的制备柱分离实验，并将其用于分离柱的标定。选择柱、泵、阀、管的尺寸用于制备分离。没有观察到由于设备尺寸增大而引起的扩散影响，实现了2个目标组分和杂质组分的分离。与传统的简单循环系统进行了比较，结果表明，该系统在收率、浓度和生产能力方面具有明显的优势。（2）可变体积管的研制在本系统中，可变体积管用作保持色谱轮廓的体积。通过改变管的长度来处理目标组分的条件的变化，例如浓度和所需产率。研制了变容管，并通过实验验证了其有效性。（3）建立了高效液相色谱模拟器的规划系统。利用粗分离后的目标混合物的色谱图来设定模拟器的参数。利用该模拟器可以对分离结果进行准确的预测。