Development of chromatography System for multi components with high-yield, high-purity, high-concentration and high-productivity

提出了一种新的用于多组分分离的高效液相色谱回收系统。该系统通过回收进料两侧的残余组分来进行加宽的干扰。该系统实现了目标成分的高纯度、高浓度、高产率。此外，还实现了洗脱液消耗的减少。在本研究中，进行了模拟和实验。(1)制备柱实验研究了用于分离柱校正的反式氧化二苯乙烯的分离。用于制备分离的柱、泵、阀、管的尺寸被选择。不受设备尺寸增大的扩散影响，实现了两个目标组分和杂质组分的分离。将该系统与传统的简单循环系统进行了比较，结果表明，该系统在产率、浓度和生产率方面都具有明显的优势。(2)变体积管的研制在该系统中，以管作为体积来保持色谱线。通过改变管子的长度来处理目标组分条件的变化，例如浓度和所需产率。建立了基于高速模拟器的规划系统，开发了用于规划管子体积和换阀时机的高效液相模拟器。对粗分离的目标混合物进行层析，以设定模拟器的参数。利用该模拟器可以对分离结果进行准确的预测。

A new HPLC recycling system for multi-component separation is proposed. This system uses the interference of broadening by recycling the residual components on both sides of the introduced feed. By using this system, the high purity, high concentration, and high yield of target components are achieved. Furthermore, reduction of the eluent consumption is achieved. In this research simulations and experiments are performed.(1)Experiments with preparative columnThe separation of trans-stilben oxide that is used for calibration of separation column is performed. The size of the column, pump, valves, tubes are chosen for preparative separation. The influence of diffusion from those enlarged size of equipments is not observed, and the separation of 2 target components and the impure component is achieved. The proposed system is compared with the conventional simple recycle system, and the results showed the obvious advantage in yield, concentration and productivity.(2)Development of tube with variable volumeIn this system, the tube is used as volume for keeping the chromatography profile. The change in the condition of target components such as the concentration and required yield is handled by changing the length of the tubes. The tube with variable volume is developed and its availability is confirmed by the experiment.(3)Establishment of planning system with high-speed simulatorThe HPLC simulator is developed for planning the volume of tube and the timing of change of the valves. The chromatography of the target mixture with roughly separated is used for setting the parameters of the simulator. By using this simulator, the accurate prediction of result of separation can be obtained.