Molecular biological Study about Recovery from hemiplagia on the model of rat cerebral thrombosis

本研究旨在探讨肌肉增强的分子生物学机制，阐明肌肉增强的相关因素。肌肉强化训练是康复医学中最重要的治疗方法之一。然而，从分子生物学的角度对康复医学中肌肉强化的研究却很少。我们从基因转录和蛋白质合成两个方面对肌肉强化训练进行了研究，发现了有效的肌肉强化方法。以往的研究大多关注肌纤维的肌肉张力或肌肉肥大特征。我们用northern印迹法研究了骨骼肌拉伸中直接早期基因c-fos和肌肉特异性转录因子myogenin的表达。在本研究中，我们使用实时RT-PCR方法检测了骨骼肌的康复效果。合成代谢类固醇处理后的骨骼肌机械生长因子的表达显著增加，重复拉伸时机械生长因子和胶质细胞源性神经营养因子的表达增加，而静态拉伸时机械生长因子和胶质细胞源性神经营养因子的表达增加。

This study is to investigate the molecular biological mechanism about muscle strengthening and to clarify the factors concerning to muscle strengthening. Muscle strengthening training is one of the most important treatment procedure in the rehabilitation medicine. However, there are very few study about muscle strengthening on rehabilitation medicine from the view point of molecular biology. We investigate the muscle strengthening training as the gene transcription and protein synthesis and discover the efficient method to muscle strengthening. Almost former researches concerned about muscle tension or muscle hypertrophy character of muscle fibers. We have studied the expression of immediate early gene "c-fos" and muscle specific transcription factor "myogenin" on stretching in the skeletal muscle by northern blotting method. In this study we examined the rehabilitative effects on skeletal muscle using real time RT-PCR method. There is significant increase of expression of mechano-growth factor on the skeletal muscle treated with anabolic steroid and the expression of mechano-growth factor and glial cell derived neurotrophic factor are increased on repetitive stretching but not on static stretching.