Molecular biological Study about Recovery from hemiplagia on the model of rat cerebral thrombosis

大鼠脑血栓模型偏瘫恢复的分子生物学研究

• 批准号: 15500367
• 负责人: IKEDA Satoshi
• 金额: $ 2.3万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15500367/
• 关键词: stroke; reorganization; neurotrophic factors; rehabilitation medicine; rat; リハビリテーション

This study is to investigate the molecular biological mechanism about muscle strengthening and to clarify the factors concerning to muscle strengthening. Muscle strengthening training is one of the most important treatment procedure in the rehabilitation medicine. However, there are very few study about muscle strengthening on rehabilitation medicine from the view point of molecular biology. We investigate the muscle strengthening training as the gene transcription and protein synthesis and discover the efficient method to muscle strengthening. Almost former researches concerned about muscle tension or muscle hypertrophy character of muscle fibers. We have studied the expression of immediate early gene "c-fos" and muscle specific transcription factor "myogenin" on stretching in the skeletal muscle by northern blotting method. In this study we examined the rehabilitative effects on skeletal muscle using real time RT-PCR method. There is significant increase of expression of mechano-growth factor on the skeletal muscle treated with anabolic steroid and the expression of mechano-growth factor and glial cell derived neurotrophic factor are increased on repetitive stretching but not on static stretching.

本研究旨在探讨肌肉强化的分子生物学机制，阐明肌肉强化的相关因素。肌肉强化训练是康复医学中最重要的治疗手段之一。然而，从分子生物学的角度对肌肉强化在康复医学上的研究却很少。从基因转录和蛋白质合成两个方面对肌肉强化训练进行研究，探索肌肉强化的有效方法。以往的研究多关注肌纤维的肌张力或肌肥大特征。用北方印迹法研究了骨骼肌即刻早期基因c-fos和肌特异性转录因子myogenin在牵张过程中的表达。本研究采用真实的实时RT-PCR方法检测了骨骼肌的康复效果。有显着增加的机械生长因子的表达在骨骼肌上的合成代谢类固醇和机械生长因子和胶质细胞源性神经营养因子的表达增加重复拉伸，而不是静态拉伸。

项目成果

Satoshi Ikeda: "Induction of myogenin mRNA in rat skeletal muscle by one-hour passive repetitive stretching"Archives of Physical Medicine and Rehabilitation. 85. 166-167 (2004)

Satoshi Ikeda：“通过一小时被动重复拉伸诱导大鼠骨骼肌中的肌生成素 mRNA”物理医学和康复档案。

Induction of myogenin mRNA in rat skeletal muscle by one-hour passive repetitive stretching

一小时被动重复拉伸诱导大鼠骨骼肌肌生成素 mRNA

• 发表时间: 2004
• 期刊: Archives of Physical Medicine and Rehabilitation 85
• 作者: 吉田 輝;野元佳子 他;吉田 輝;Satoshi Ikeda
• 通讯作者: Satoshi Ikeda

池田 聡: "光感受性色素ローズベンガルを用いた脳血栓片麻痺モデルラットの作製"鹿児島リハビリテーション医学会誌. 14. 1-4 (2003)

池田聪：“使用光敏染料Rose Bengal制作脑血栓偏瘫模型大鼠”鹿儿岛康复医学会杂志14. 1-4 (2003)。

光感受性色素ローズベンガルを用いた脳血栓片麻痺モデルラットの作成

光敏染料玫瑰红建立脑血栓偏瘫模型大鼠

• 发表时间: 2003
• 期刊: 鹿児島リハビリテーション医学研究会会誌 14巻
• 作者: Kawahira;K;et al.;池田 聡
• 通讯作者: 池田 聡

Muscle weakness from bed ridden and mechanism of muscle strengthening

卧床肌无力及其强化机制

• 期刊: Kagoshima medical assosiation journal 639
• 作者: 野口裕美;原 和子;國見ゆみ子;安藤徳彦;Satoshi IKEDA Nobuyuki TANAKA
• 通讯作者: Satoshi IKEDA Nobuyuki TANAKA