Development of Effective Analytical System for Studies on Human Exposure to Toxic Elements Caused by Environmental Pollution

2003财政年度，建立了一种未经处理的指甲样本的无标准方法，并已在菲律宾和蒙古等多个国家应用于人体汞和砷暴露的研究。此外，我们还利用两年前建立的“三探测器测量系统”开发了一种定量分析氟的方法。这种方法使我们能够讨论氟与其他有毒元素之间的关系，如砷和锰。将该方法应用于污染地区中国人样品的测定，证实了氟、锰、砷之间的相关性。该方法已进一步应用于植物、海产品、真菌和自来水等领域，成为环境科学和地理学等领域的一种强有力的方法。2004财年，购买了一台新的外部多道分析仪，建立了三个探测器光谱数据的有效分析系统。该方法已应用于中国、莫戈利亚、哈萨克斯坦等污染地区人群的2000多份毛发、尿液和指甲的分析，并建立了脊髓液、唾液、汗液、泪水和鼻涕的无标样分析方法，使对极少量的样品进行定量分析成为可能。我们正计划将这些方法应用于亚洲许多国家对人类接触有毒元素的研究。

In 2003 fiscal year, a standard-free method for untreated nail samples was established, and it has been applied to studies on human exposure to mercury and arsenic in various countries such as Philippines and Mongolia. Further, we developed a method of quantitative analysis of fluorine making use of the "Three-detector measuring system" which we established two years ago. This method allows us to discuss correlation between fluorine and other toxic elements such as arsenic and manganese. This method has been applied to the samples taken from Chinese people living in contaminated areas, and the correlation among fluorine, manganese and arsenic were confirmed. This method has been further applied to plants, sea foods, fungi, and tap waters, and becomes a powerful method in the fields of environmental science and sitology etc.In 2004 fiscal year, a new external multi-channel analyzer was purchased and the system for effective analyses of spectrum data from three detectors was established. It has been applied to more than 2000 hairs, urines and nails taken from people living in contaminated areas in China, Monogolia and Kazakhstan etc. Furthermore, Standard-free methods for spinal fluid, saliva, sweat, tear and snivel have been developed and it made it possible to quantitatively analyze extremely small amount of these samples. We are planning to apply these methods to studies on human exposure to toxic elements in many countries in Asia.