Morphological and molecular evolution of Radiolaria based on the 18S rDNA study

基于18S rDNA研究的放射虫形态和分子进化

• 批准号: 15540449
• 负责人: TAKAHASHI Osamu
• 金额: $ 2.37万
• 依托单位: Tokyo Gakugei University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15540449/
• 关键词: Polycystinea; Acantharea; Phaeodarea; Nassellarida; Spumellarida; 18S rDNA; molecular phylogeny; Radiolaria; Spumellaria; Nassellaria; 18SrDNA; 系統樹; 分子糸統

The marine, holoplanktonic protists group called Phaeodarea is today represented by several hundred species. The phaeodarian skeletons consist of opaline silica as well as organic matter, and are very fragile and vulnerable to dissolution. Their tests are therefore rarely found in the fossil record, and this has caused much uncertainty around their phylogenetic evolution. In this study, three small, solitary phaeodarian species; Protocystis xiphodon (Haeckel), Challengeron diodon Haeckel, and Conchellium capsula Borgert were examined using molecular techniques in order to clarify the phylogenetic position of the Phaeodarea. The obtained phylogenetic trees for all analyses, the neighbor-joining, maximum-parsimony, and maximum-likelihood methods, combined with sequences of Polet et al. (2004) from large phaeodarian species, showed that the all phaeodarians formed a monophyletic group within Phylum Cercozoa. This result run counters to Haeckel's classical taxonomy grouping the phaeodarians together with the polycystines (i.e., nassellarians and spumellarians) and the achantharians, under the common name Radiolaria, and suggesting a monophyly for these groups. Our results strongly suggested the polyphyly of Radiolaria.

被称为Phaeodarea的海洋全浮游原生生物群今天有几百个物种。褐藻骨架由蛋白石二氧化硅和有机物组成，非常脆弱，容易溶解。因此，他们的测试很少在化石记录中找到，这给他们的系统发育进化带来了很多不确定性。在本研究中，有三种小型、独居的食虫动物；利用分子技术对剑剑原囊藻（procystis xiphodon (Haeckel)）、棘囊藻（Challengeron diodon Haeckel）和贝壳藻（Conchellium capsula Borgert）进行了系统发育鉴定。所有分析的系统发育树，邻居连接法，最大简约法和最大似然法，结合Polet et al.（2004）对大型食腐动物物种的序列，表明所有食腐动物在尾藻门内形成了一个单系群。这一结果与海克尔的经典分类学背道而驰。海克尔将phaeodarians与多胱胺类（即鼻虫类和刺虫类）和无卵虫类归在一起，共同命名为放射虫类，并表明这些群体是单一的。本研究结果强烈提示放射菌属存在多聚性。

项目成果

Phylogenetic analysis of the polycystine Radiolaria based on the 18S rDNA sequences of the Spumellarida and the Nassellarida.

基于 Spumellarida 和 Nassellarida 的 18S rDNA 序列对多胱氨酸放射虫进行系统发育分析。

• 发表时间: 2005
• 期刊: European Journal of Protistology 41-1
• 作者: Yuasa;T. et al.
• 通讯作者: T. et al.

Phaeodarian Radiolaria from the Upper Cretaceous beds of the central Japan

来自日本中部上白垩纪地层的斐奥达放射虫

• 发表时间: 2004
• 期刊: Revue de micropaleontologie 47-3
• 作者: Takahashi;O.;Yuasa;T.;Honda;D.;Mayama;S.;O.Takahashi
• 通讯作者: O.Takahashi

PCR primers for the amplification of the nuclear small subunit ribosomal DNA sequences from polycystine radiolarians.

用于扩增多胱氨酸放射虫核小亚基核糖体 DNA 序列的 PCR 引物。

• 发表时间: 2004
• 期刊: Japanese Journal of Protozoology 37
• 作者: Yuasa;T. et al.
• 通讯作者: T. et al.

Takahashi, O., Mayama, S., Matsuoka A.: "Host-symbiont assocition of polycystine Radiolaria : epifluoresence microscopic observation of living Radiolaria"Marine Micropaleontlogy. 49-3. 187-194 (2003)

高桥，O.，真山，S.，松冈A.：“多胱氨酸放射虫的宿主共生关联：活体放射虫的落射荧光显微镜观察”海洋微古生物学。

Takahashi, O., Yuasa, T., Honda, D., Mayama, S.: "Molecular phylogeny of the solitary shell-bearing Polycystinea (Radiolaria)"Revue de Micropaleontlogie. 47-2(in press). (2004)

Takahashi, O.、Yuasa, T.、Honda, D.、Mayama, S.：“孤生壳多囊藻（放射虫）的分子系统发育”Revue de Micropaleontlogie。