A study of fiber connections controlling jaw movement and salivary secretion with the use of mmnohistochemical and in situ hybridization technique

GABA和甘氨酸被认为是主要的抑制性神经递质。本研究观察了大鼠三叉神经运动核（Vm）内GABA_A受体样免疫反应（R-LI）、GABA_B受体样免疫反应（GABAB-R-LI）和甘氨酸受体样免疫反应（Glycine-R-LI）在生后发育过程中的共定位。实验在0 - 35日龄雌性Wistar大鼠上进行。用激光共聚焦显微镜和电子衍射显微镜观察GABA-R-LI和Gly-R-LI的表达。7日龄时，Gly-R-LI分布在Vm的背外侧部分聚集。GABA_B-R-LI阳性细胞主要分布于胞体和Vm运动神经元近端树突的细胞膜上，部分胞体也被GABA_B-R-LI标记。28 ~ 35日龄时，Vm神经元胞体内Gly-R-LI几乎消失，但几乎所有运动神经元上仍可见GABA_A-R-LI和GABA_B-R-LI。这些结果表明，抑制性神经递质的受体转移其分布在哺乳的过渡阶段的大鼠咬的行为。

GABA and glycine are thought to be major inhibitory neurotransmitters. This study was undertaken to demonstrate co-localization of GABA_A receptor-like immunoreactive (R-LI), GABA_B-R-LI and glycine-R-LI profiles in the rat trigeminal motor nucleus (Vm) during the course of their postnatal development. Experiments were carried out on female Wistar rats of 0 to 35 days old. GABA-R-LI and Gly-R-LI profiles were detected immunocytochemically with the use confocal laser scanning microscope and electron spectroscopic diffraction microscope. At 7 days old Gly-R-LI profiles were aggregated in the dorsolateral part of Vm. They were seen on the cell membrane of the somata and the proximal dendrites of a Vm motoneuron, and some cell bodies were also labeled with GABA_B-R-LI. In 28 to 35 days old animals Gly-R-LI was almost disappeared in the confine of Vm but GABA_A-R-LI and GABA_B-R-LI were still observed on almost all motoneurons of Vm neuronal cell bodies. These results suggest that receptors for inhibitory neurotransmitters shift their distribution in the transition phase of suckling to biting behavior of the rat.