A STUDY OF NEURONAL CIRCUIT FOR JAW MOVEMENTS,WITH IMMUNOHISTOCHEMICAL AND IN SITU HYBRIDIZATION TECHNIQUE.

最近，利用谷氨酰胺酶（一种谷氨酸合成酶）对大脑中含有谷氨酸的神经元进行了研究。利用抗谷氨酸多克隆抗体对三叉神经运动核和感觉核中的谷氨酸能神经元细胞体及其末端进行鉴定。本研究采用雌性Wistar大鼠。深度麻醉后灌注2.0%多聚甲醛和0.25%戊二醛。立即切除脑干，连续冰冻切片。用异硫氰酸荧光素（FITC）对谷氨酸免疫反应神经元细胞体和静脉曲张进行免疫组化标记，并在荧光显微镜下观察。采用免疫荧光技术检测谷氨酸免疫反应（Glu-IR）细胞体和细胞曲张。在三叉神经运动核（Vm）内，在运动神经元及其近端树突上观察到少量的Glu-IR变异。三叉神经脊髓核（Vsp）尾侧亚核（Vc）内可见密集的Glu-IR纤维，三叉神经主感觉核（Vp）和Vsp极间亚核（Vi）内可见大量Glu-IR细胞。在Vsp附近和Vm周围的parvocellular reticular nucleus （pcRN）中也观察到大量的Glu-IR细胞。

Recently a study of glutamic acid containing neurons in the brain have been carried out with the use of glutaminase, a synthetic enzyme of glutamic acid. This study was undertaken to identify the glutamatergic neuronal cell bodies and their terminals in the trigeminal motor nucleus and the trigeminal sensory nuclei, with the use of polyclonal antibody against glutamic acid.Female Wistar rats were used for this study. They were anesthetized deeply and were perfused with 2.0% paraformaldehyde and 0.25% glutaraldehyde. The brain stem was removed immediately and serial frozen sections were made. Immunohistochemical labeling of glutamate - immunoreactive neuronal cell bodies and varicosities were performed with fluorescein isothiocyanate (FITC), and were observed under a fluorescent microscope.Glutamate-immunoreactive (Glu-IR) cell bodies and varicosities were detected by immunofluorescence technique. In the confine of cytoarchitechtonicaliy defined trigeminal motor nucleus (Vm), a few Glu-IR varicosities were observed on motoneurons and their proximal dendrites. Dense Glu-IR fibers were seen in the caudal subnucleus (Vc) of the spinal trigeminal nucleus (Vsp), and a number of Glu-IR celis were encountered in the trigminal main sensory nucleus (Vp) and the interpolar subnucleus (Vi) of Vsp. A number of Glu-IR cells were also observed in the parvocellular reticular nucleus (pcRN) adjacent to Vsp and around Vm.