权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of A Sensing System for Endocrine Disrupting Chemicals Utilizing Nano Chemical Receptor Modified Sensor Chip

利用纳米化学受体修饰的传感器芯片开发内分泌干扰化学品传感系统

基本信息

批准号：
15550130
负责人：
KUBO Izumi
金额：
$ 2.05万
依托单位：
Soka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this study, molecularly imprinted polymer (MIP) receptor was utilized as a molecular recognition element of chemical sensor based on electrochemical detection of endocrine disrupting chemicals (EDCs). Triazine herbicides such as atrazine and cimazine (CAT) were chosen as EDCs, because they are widely used in agricultural field and/or golf course. In order to prepare MIP receptor, CAT was used as a template molecule and methacrylic acid (MAA) and ethylenedimethacrylate (EDMA) were used as functional monomer and cross linking monomer, respectively.NMR studies demonstrated that the chemical shifts of carboxyl proton in MAA and amino proton in CAT interacted in chlorofolm and DMF. And these chemical shfts were inclinced as to the ratio of MAA/CAT. The inclination reached steady till the MAA/CAT came to around 7. Thus seven MAA molecules would interact with one CAT molecule in chloroform and DMF.CAT-MIP was prepared in DMF or chloroform. CAT-MIP prepared in chloroform showed no difference in the re-binding of CAT with non-MIP. However, CAT-MIP prepared in DMF showed its binding of more amount than non-MIP and it was served to the selective binding assay. CAT-MIP showed selective binding to CAT among other triazines(atrazine and propazine).Electrochemical detection of CAT was performed with gold or amalgamated gold electrode. Amalgamated gold electrode showed higher electrochemical response to CAT than a gold electrode. CAT recuction was observed at pH 3-5. At higher pH o reduction current was observed and at lower pH, proton recuction current overwhelmed the CAT reduction. At pH 3, the maximum reductive current of CAT was observed with an amalgamated gold electrode. CAT sample was bound to MIP and eluted by Methanol and it was detedted with the electrode. The detection was selective to CAT.

本研究利用分子印迹聚合物(MIP)受体作为电化学检测内分泌干扰物(EDCs)的化学传感器的分子识别元件。三嗪类除草剂如阿特拉津和西马津(CAT)因其在农田和高尔夫球场上的广泛应用而被选为除草剂。为了制备MIP受体，以CAT为模板分子，甲基丙烯酸(MAA)为功能单体，二甲基丙烯酸乙二醇酯(EDMA)为交联剂，核磁共振研究表明MAA中的羧基质子和CAT中的氨基质子在氯仿和DMF中发生化学位移。这些化学位移与MAA/CAT的比例有关。在氯仿中，7个MAA分子与1个CAT分子相互作用，在DMF或氯仿中制备了CAT-MIP。在氯仿中制备的CAT-MIP对CAT与非MIP的再结合没有影响。而在DMF中制备的CAT-MIP显示出比非MIP更多的结合量，并用于选择性结合实验。CAT-MIP与其他三嗪类化合物(阿特拉津和异丙嗪)有选择性结合。CAT的电化学检测采用金电极或金电极。与金电极相比，金电极对过氧化氢具有更高的电化学响应。在pH值为3-5的条件下可观察到过氧化氢酶的还原。在较高pH值下观察到还原电流，而在较低pH值时，质子吸收电流抑制了CAT的还原。在pH=3的条件下，金电极的还原电流最大。将CAT样品与MIP结合，用甲醇洗脱，用电极进行检测。该检测方法对CAT具有选择性。