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Genomic analysis of Drosophila ananassae and its sibling species by means of parthenogenetic hybrid

通过孤雌杂交对果蝇及其同胞物种进行基因组分析

基本信息

批准号：
15570002
负责人：
SAWAMURA Kyoichi
金额：
$ 2.37万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Hybrids were made from the cross of parthenogenetic strains of Drosophila ananassae (a unisexual impaternate strain females) and D.pallidosa (a bisexual bridge strain males). As the former has a visible marker yellow on XL (the left arm of the X chromosome ; hereafter chromosome arms are abbreviated similarly (L, the left arm ; R, the right arm)), hybridism is confirmed by the yellow^+ phenotype of the females. The F_1 females produce offspring parthenogenetically and 266 lines were established from individual F_2 females. The lines have various genotypes with interspecific mosaic genomes which are made homozygous (i.e., iso-genic). We investigated the genomic structure (the species origin of every chromosome region) of each mosaic genome lines. Chromosome inversions In(3R)A of D.ananassae and In(XL)A, In(2L)CD+B and In (3R)B of D. pallidosa were utilized for cytological markers. 6 PCR-RFLP markers whose cytological locations have been determined (w (XR), Om (2D)(2mid), eyg (2R), cn (3L), Amyrel (3mid) and RfaBp (4)) plus 2 RAPD markers completely linked to the above-mentioned inversions (OPA15-950 (2L) and OPA15-1200 (3R)) were also utilized. The genomic structure of all the lines has been determined. The statistical test indicated quasi-linkage between 2L and 3R. This might be caused by either (1)low fitness (fertility or viability) of some genotypes or (2)simultaneous distribution of the chromosomes of the same species origin in meiosis. We are going to use the interspecific mosaic genome lines established here for the mapping of genes determining species-specific characters (including sexual behavior).

用单性无父系果蝇孤雌生殖品系与两性桥果蝇品系杂交，获得杂交后代。由于前者在XL(X染色体的左臂；此后染色体臂缩写为类似的缩写(L，左臂；R，右臂)上具有可见的标记黄色)，因此，雌性的黄色^+表型证实了杂交。F_1代以孤雌生殖方式产生后代，由F_2代雌性单株建立了266个品系。这些品系具有不同的基因类型，其种间马赛克基因组是纯合的(即等基因)。我们调查了每个花叶基因组系的基因组结构(每个染色体区域的物种起源)。利用(3R)A和(XL)A、(2L)Cd+B和In(3R)B染色体倒位作为细胞学标记。还利用了6个细胞学位置已确定的PCR-RFLP标记(w(XR)、Om(2D)(2MID)、EYG(2R)、CN(3L)、Amyrel(3Mid)和RfaBp(4))和2个与上述倒位完全连锁的RAPD标记(OPA15-950(2L)和OPA15-1200(3R))。所有品系的基因组结构都已确定。统计检验表明，2L和3R之间存在准连锁。这可能是由于(1)某些基因类型的适合度低(育性或活力)或(2)在减数分裂过程中起源于同一物种的染色体同时分布所致。我们将使用这里建立的种间马赛克基因组系来绘制决定物种特有特征(包括性行为)的基因图谱。