Genomic analysis of Drosophila ananassae and its sibling species by means of parthenogenetic hybrid

通过孤雌杂交对果蝇及其同胞物种进行基因组分析

基本信息

批准号：
15570002
负责人：
SAWAMURA Kyoichi
金额：
$ 2.37万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Hybrids were made from the cross of parthenogenetic strains of Drosophila ananassae (a unisexual impaternate strain females) and D.pallidosa (a bisexual bridge strain males). As the former has a visible marker yellow on XL (the left arm of the X chromosome ; hereafter chromosome arms are abbreviated similarly (L, the left arm ; R, the right arm)), hybridism is confirmed by the yellow^+ phenotype of the females. The F_1 females produce offspring parthenogenetically and 266 lines were established from individual F_2 females. The lines have various genotypes with interspecific mosaic genomes which are made homozygous (i.e., iso-genic). We investigated the genomic structure (the species origin of every chromosome region) of each mosaic genome lines. Chromosome inversions In(3R)A of D.ananassae and In(XL)A, In(2L)CD+B and In (3R)B of D. pallidosa were utilized for cytological markers. 6 PCR-RFLP markers whose cytological locations have been determined (w (XR), Om (2D)(2mid), eyg (2R), cn (3L), Amyrel (3mid) and RfaBp (4)) plus 2 RAPD markers completely linked to the above-mentioned inversions (OPA15-950 (2L) and OPA15-1200 (3R)) were also utilized. The genomic structure of all the lines has been determined. The statistical test indicated quasi-linkage between 2L and 3R. This might be caused by either (1)low fitness (fertility or viability) of some genotypes or (2)simultaneous distribution of the chromosomes of the same species origin in meiosis. We are going to use the interspecific mosaic genome lines established here for the mapping of genes determining species-specific characters (including sexual behavior).

杂种是由果蝇ananassae（单性的沉闷菌株雌性）和d.pallidosa（双性恋桥菌株雄性）的孤子发育菌株的十字架制成的。由于前者在XL上有明显的标记（X染色体的左臂；以后的染色体臂也被缩写为类似的（L，左臂； R，右臂）），女性的黄色^+表型证实了杂交。 F_1女性在遗传学上产生后代，并从单个F_2女性中建立了266条线。这些线具有各种基因型，具有种间镶嵌基因组，这些基因组是纯合的（即同基因）。我们研究了每个镶嵌基因组系的基因组结构（每个染色体区域的物种起源）。 d.ananassae和（xl）a的（3r）A中的染色体反转（2L）cd+b和（3r）b的（3r）B中用于细胞学标志物。 6个PCR-RFLP标记，其细胞学位置已确定（W（XR），OM（2d）（2MID），EYG（2R），CN（3L），Amyrel（3MID）和RFABP（4）和2个RAPD标记，以及2个完全链接到上述反逆上的RAPD标记（OPA15-950（OPA15-950（2L）和OPLILINITION（2L）和OPLILILIDILILION EPLILINILILION IPARITION和OPLILILINIT UPILILINILILINIT（3L）和OPERIP。所有线的基因组结构已确定。统计检验表明2L和3R之间的准链接。这可能是由于（1）某些基因型的低适应性（生育能力或生存力）或（2）同一物种起源在减数分裂中的染色体的同时分布。我们将使用此处建立的种间镶嵌基因组线，以绘制确定物种特异性特征（包括性行为）的基因。