Cloning of genes controlling quail plumage pigmentation and their expression

鹌鹑羽毛色素控制基因的克隆及其表达

• 批准号: 15570173
• 负责人: SHIOJIRI Nobuyoshi
• 金额: $ 2.05万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570173/
• 关键词: Feather; Coloration pattern; Black at hatch; Quail; Melanocytes; GDRDA; 黒色初毛致死変異; 羽毛芽; 発生遺伝学

The present study with quail Bh (black at hatch) mutants was undertaken to reveal molecular mechanisms of pattern formation in plumage pigmentation. The following conclusions were obtained from the studies carried out in 2003 and 2004 :1.Quail cDNAs for α-MSH, its receptor (MC1R) and agouti protein were cloned. Although the localization of their mRNAs is not related to the plumage pigmentation pattern, α-MSH produced in epidermal cells can work on melanocytes expressing MC1R in feather germs, leading to melanin production.2.The Bh gene maps to the long-arm of chromosome 1 (FISH analysis).3.Bioinformatics analysis using the chicken genome and linkage analysis in Bh quails showed that the Bh gene may be located between MHD1B3 and Q1H3orf1 in chromosome 1.

本研究以鹌鹑Bh（black at hatch）突变体为研究对象，旨在揭示羽毛色素形成模式的分子机制。通过2003年和2004年的研究，获得以下主要结论：1.克隆了鹌鹑α-MSH及其受体（MC 1 R）和agglutinin蛋白的cDNA。虽然它们的mRNA定位与羽毛色素沉着模式无关，但表皮细胞产生的α-MSH可以作用于羽胚中表达MC 1 R的黑素细胞，导致黑色素的产生。2. Bh基因定位于1号染色体的长臂（FISH分析）3.利用鸡基因组进行生物信息学分析和Bh鹌鹑的连锁分析表明，Bh基因可能位于MHD 1B 3和Q1 H3 orf 1之间。在1号染色体上。

项目成果

Niwa, T., Shibusawa, M., Matsuda, Y., Terashima, A., Nakamura, A., Shiojiri, N.: "The Bh (black at hatch) gene that causes abnormal feather pigmentation maps to chromosome 1 of the Japanese quail"Pigment Cell Research. 16. 656-661 (2003)

Niwa, T.、Shibusawa, M.、Matsuda, Y.、Terashima, A.、Nakamura, A.、Shiojiri, N.：“引起异常羽毛色素沉着的 Bh（孵化时黑色）基因映射到羽毛色素的 1 号染色体上。

Bh (black at hatch) gene that causes abnormal feather pigmentation was mapped on chromosome 1 of Japanese quail

导致羽毛色素异常沉着的 Bh（孵化时黑色）基因被定位在日本鹌鹑 1 号染色体上

• 发表时间: 2003
• 期刊: Pigment Cell Research 16
• 作者: Masami Nagahama;Masami Nagahama;T.Niwa et al.;T.Niwa et al.
• 通讯作者: T.Niwa et al.

Bh (black at hatch) gene that causes abnormal feather pigmentation was mapped on chromosome 1 Japanese quail

导致羽毛色素异常沉着的 Bh（孵化时黑色）基因被定位在日本鹌鹑 1 号染色体上

• 发表时间: 2003
• 期刊: Pigment Cell Research 16
• 作者: Masami Nagahama;Masami Nagahama;T.Niwa et al.
• 通讯作者: T.Niwa et al.