Cloning of genes controlling quail plumage pigmentation and their expression

鹌鹑羽毛色素控制基因的克隆及其表达

• 批准号: 15570173
• 负责人: SHIOJIRI Nobuyoshi
• 金额: $ 2.05万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570173/
• 关键词: Feather; Coloration pattern; Black at hatch; Quail; Melanocytes; GDRDA; 黒色初毛致死変異; 羽毛芽; 発生遺伝学

The present study with quail Bh (black at hatch) mutants was undertaken to reveal molecular mechanisms of pattern formation in plumage pigmentation. The following conclusions were obtained from the studies carried out in 2003 and 2004 :1.Quail cDNAs for α-MSH, its receptor (MC1R) and agouti protein were cloned. Although the localization of their mRNAs is not related to the plumage pigmentation pattern, α-MSH produced in epidermal cells can work on melanocytes expressing MC1R in feather germs, leading to melanin production.2.The Bh gene maps to the long-arm of chromosome 1 (FISH analysis).3.Bioinformatics analysis using the chicken genome and linkage analysis in Bh quails showed that the Bh gene may be located between MHD1B3 and Q1H3orf1 in chromosome 1.

本研究用鹌鹑BH（孵化时为黑色）突变体进行了揭示羽毛色素沉着中模式形成的分子机制。从2003年和2004年进行的研究中得出以下结论：1。α-MSH的Quail cDNA，其受体（MC1R）和Agouti蛋白是克隆的。尽管其MRNA的定位与羽毛色素沉着模式无关，但在表皮细胞中产生的α-MSH会引起梅拉曲线的造成粉丝MC1。 BH基因映射到1染色体1的长臂（鱼类分析）。3。使用鸡基因组和BH鹌鹑中的链接分析的生物信息学分析表明，BH基因可能位于染色体1中的MHD1B3和Q1H3ORF1之间。

项目成果

Niwa, T., Shibusawa, M., Matsuda, Y., Terashima, A., Nakamura, A., Shiojiri, N.: "The Bh (black at hatch) gene that causes abnormal feather pigmentation maps to chromosome 1 of the Japanese quail"Pigment Cell Research. 16. 656-661 (2003)

Niwa, T.、Shibusawa, M.、Matsuda, Y.、Terashima, A.、Nakamura, A.、Shiojiri, N.：“引起异常羽毛色素沉着的 Bh（孵化时黑色）基因映射到羽毛色素的 1 号染色体上。

Bh (black at hatch) gene that causes abnormal feather pigmentation was mapped on chromosome 1 of Japanese quail

导致羽毛色素异常沉着的 Bh（孵化时黑色）基因被定位在日本鹌鹑 1 号染色体上

• 发表时间: 2003
• 期刊: Pigment Cell Research 16
• 作者: Masami Nagahama;Masami Nagahama;T.Niwa et al.;T.Niwa et al.
• 通讯作者: T.Niwa et al.

Bh (black at hatch) gene that causes abnormal feather pigmentation was mapped on chromosome 1 Japanese quail

导致羽毛色素异常沉着的 Bh（孵化时黑色）基因被定位在日本鹌鹑 1 号染色体上

• 发表时间: 2003
• 期刊: Pigment Cell Research 16
• 作者: Masami Nagahama;Masami Nagahama;T.Niwa et al.
• 通讯作者: T.Niwa et al.