Shizuoka University Developmental genetics on mechanisms of plumage pigmentation in quails

静冈大学 鹌鹑羽毛色素沉着机制的发育遗传学

• 批准号: 12680711
• 负责人: SHIOJIRI Nobuyoshi
• 金额: $ 2.3万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680711/
• 关键词: Feather; Pigmentation; Black at hatch; Quail; Melanocytes; GDRDA; 羽毛色素パターン; Bh; 変異体; 神経冠; 初代培養; 黒色初毛致死突然変異; メラノブラスト; 羽毛芽色素パターン; Mitf; 黒色初毛致死突然変異体; 遺伝子発現; ニホンウズラ

The present study with quail Bh (black at hatch) mutants isolated some genetic markers linked to the Bh locus, histochemically analyzed the development of the longitudinal black and yellow stripes of pigments in the embryonic dorsal plumages, and led to the following conclusions :1. GDRDA allowed us to isolate three genetic markers linked to the Bh locus, and the approximate genetic distances between the Bh locus and each marker locus are 1-14 cM. Genotyping methods using PCR and a restriction enzyme were established for those genetic markers.2. MelEM antigen was expressed in melanoblasts committed to produce black pigment before apparent melanogenic gene expression, and Bh heterozygotes and homozygotes showed abnormal expression patterns of the MelEM antigen. These results indicate that MelEM antigen is a good marker for melanoblasts committed to produce black pigment, and suggest that the Bh locus directs melanocytes to produce eumelanin in proper position.

本研究分离了一些与Bh基因座连锁的遗传标记，并对胚胎背部羽毛中黑色和黄色纵纹的发育进行了组织化学分析，得到以下结论：1.GDRDA允许我们分离到3个与Bh基因座连锁的遗传标记，Bh基因座与每个标记座位之间的遗传距离约为1-14 cM。建立了利用聚合酶链式反应和限制性内切酶对这些遗传标记进行基因分型的方法。在黑素生成基因表达之前，MelEM抗原在致力于产生黑色素的黑素母细胞中表达，而Bh杂合子和纯合子则表现出MelEM抗原的异常表达模式。这些结果表明，MelEM抗原是致力于产生黑色素的黑素母细胞的良好标记物，并提示Bh基因座引导黑素细胞在适当的位置产生真黑素。

项目成果

Niwa, T. et al.: "Plumage pigmentation and expression of its regulatory genes during quail development"Mechanisms of Development. 118. 139-146 (2002)

Niwa, T. 等人：“鹌鹑发育过程中羽毛色素沉着及其调控基因的表达”发育机制。

Niwa, Tohru: "Plumage pigmentation and expression of its regulating genes during quail development"Mechanisms of Development. 118. 139-146 (2002)

Niwa, Tohru：“鹌鹑发育过程中羽毛色素沉着及其调节基因的表达”发育机制。

Niwa, T. et al.: "Plumage Pigmentation and expression of its regulatory genes during quail development"Mechanisms of Development. Vol.118. 139-146 (2002)

Niwa, T. 等人：“鹌鹑发育过程中羽毛色素沉着及其调控基因的表达”发育机制。