Mosaic analysis of cell lineages in mouse liver

小鼠肝脏细胞谱系的镶嵌分析

• 批准号: 09680722
• 负责人: SHIOJIRI Nobuyoshi
• 金额: $ 2.11万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680722/
• 关键词: Mosaic mouse; Ornithine transcarbamylase; Liver; Cell image; Bile cfucts; 性モザイクマウス; オルニチルトランスカルバミラーゼ; グルタミン合成酵素

The present study with the spfィイD1ashィエD1-heterozygous female mouse analyzed the cell lineages in the liver and small intestine, and leads to the following conclusions:1. The mosaic pattern in the heterozygous liver is not related to the liver anatomy such as the liver lobule. This result suggests that hepatocytes do not migrate straight from the portal area to central area. Hepatocytes allocate their daughter cells randomly and coherently.2. There are three types of hepatoblasts in their cell lineages: hepatoblasts giving rise to only mature hepatocytes, hepatoblasts giving rise to only biliary epithelial cells, and hepatoblasts giving rise to both cells.3. The crypt formation in the small intestine starts at the neonatal period, but the immature crypt was not monoclonal. The monoclonal crypts were established in 2-weeks-old small intestine.4. Patches in fetal liver and small intestine were very small, suggesting that extensive cell mixing and migration take place at these periods.

本研究以spfィイD1ashエD1杂合雌性小鼠为对象，分析了其肝脏和小肠的细胞谱系，得出以下结论： 1．杂合子肝脏中的镶嵌图案与肝小叶等肝脏解剖结构无关。该结果表明肝细胞不会直接从汇管区迁移到中心区。肝细胞随机、一致地分配其子细胞。 2．成肝细胞的细胞谱系分为三种类型：仅产生成熟肝细胞的成肝细胞、仅产生胆管上皮细胞的成肝细胞、以及产生两种细胞的成肝细胞。3．小肠中的隐窝形成始于新生儿期，但未成熟的隐窝不是单克隆的。 2周龄小肠内建立单克隆隐窝。 4．胎儿肝脏和小肠中的斑块非常小，表明在这些时期发生了广泛的细胞混合和迁移。

项目成果

Shiejiri, N., Sane, M., Inujima, S., Nitou, M., Kanazawa M. and Mori, M.: "Quantitative analysis of cell allocation during liver development using the spfィイD1ashィエD1-heterozygous female mouse"American Journal of Pathology. 156(1). 65-75 (2000)

Shiejiri, N.、Sane, M.、Inujima, S.、Nitou, M.、Kanazawa M. 和 Mori, M.：“使用 spfiiD1ashieD1 杂合雌性小鼠对肝脏发育过程中的细胞分配进行定量分析”美国病理学杂志156（1）。65-75（2000）。

N.Shiojiri et al.: "Quantitative analysis of cell allocation during liver development, using the splash-heterozygous female mouse"American Journal of Pathology. 156(1). 65-75 (2000)

N.Shiojiri 等人：“使用飞溅杂合雌性小鼠对肝脏发育过程中的细胞分配进行定量分析”美国病理学杂志。

N.Shiojiri et al.: "Quantitative analysis of cell allocation during liver development,using the spf-ash-heterozygous female mouse"American Journal of Pathology. 156(1). 65-75 (2000)

N.Shiojiri 等人：“使用 spf-ash 杂合雌性小鼠对肝脏发育过程中的细胞分配进行定量分析”美国病理学杂志。