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Gene expression analysis of intestinal intraepithelial lymphocytes derived from mice fed a food antigen or functional food factors.

对饲喂食物抗原或功能性食物因子的小鼠肠上皮内淋巴细胞进行基因表达分析。

基本信息

批准号：
15580101
负责人：
TOTSUKA Mamoru
金额：
$ 2.3万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We focused on intestinal intraepithelial lymphocytes (IEL), which are a kind of T cells residing in intestinal epithelia, and the gene expression in IEL derived from mice fed a food antigen was analyzed. When ovalbumin-specific T-cell receptor transgenic mice (DO11.10 mice) were fed a diet containing egg white proteins (EW-diet) ad libitum for 3 days, increase in the number of CD4^+ IEL among whole IEL and alteration of cell-surface molecule expression on IEL were observed. DNA microarray analysis of IEL derived from mice fed the EW-diet showed the increased expression of 98 genes and decreased expression of 24 genes. Genes with increased expression contained cell-cycle related genes and immune-related genes including interleukin-10 (IL-10). IEL derived from the EW-fed mice showed increased secretion of IL-10 compared to the control IEL. IL-10 gene expression was significantly observed in CD4^+ IEL subset without in vitro stimulation, and the expression level was higher than CD4^+ T cells from other gut-associated lymphoid tissues. Since IL-10 gene expression per CD4^+ IEL was not increased by feeding the EW-diet, the increased cell number of CD4^+ IEL subsets was suggested to be responsible for the increased gene expression of IL-10 in IEL from the EW-fed mice. When the gene expression profiles were compared among IEL subsets, the expression of granzyme A was shown to be much less in CD4^+ IEL than in other IEL subsets. Since IL-10 is a cytokine important for the immune-suppressive function of a kind of regulatory T cells, CD4^+ IEL may have a function in regulating the immune responses in intestinal mucosa, and the regulatory function of IEL would be increased by feeding a food antigen.

我们专注于肠上皮内淋巴细胞（IEL），这是一种存在于肠上皮细胞中的T细胞，并分析了来自喂食食物抗原的小鼠的IEL中的基因表达。当卵清蛋白特异性T细胞受体转基因小鼠（DO11.10小鼠）自由采食含有鸡蛋白色蛋白的饲料（EW饲料）3天后，观察到整个IEL中CD 4 ^+ IEL数量增加，IEL上细胞表面分子表达改变。来自饲喂EW饮食的小鼠的IEL的DNA微阵列分析显示98个基因的表达增加和24个基因的表达减少。表达增加的基因包括细胞周期相关基因和免疫相关基因，包括白细胞介素-10（IL-10）。与对照IEL相比，来自EW喂养小鼠的IEL显示IL-10分泌增加。IL-10基因在未经体外刺激的CD 4 ^+ IEL亚群中表达显著，且表达水平高于其他肠道相关淋巴组织中的CD 4 ^+ T细胞。由于饲喂EW饲料并没有增加每个CD 4 ^+ IEL中IL-10的基因表达，因此，CD 4 ^+ IEL亚群细胞数量的增加被认为是EW喂养小鼠IEL中IL-10基因表达增加的原因。当比较IEL亚群的基因表达谱时，颗粒酶A的表达在CD 4 ^+ IEL中比在其他IEL亚群中少得多。由于IL-10是一种对调节性T细胞的免疫抑制功能重要的细胞因子，因此CD 4 ^+ IEL可能具有调节肠粘膜免疫反应的功能，并且通过喂食食物抗原可以增强IEL的调节功能。

项目成果

期刊论文数量（52）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Dendritic cells from spleen, mesenteric lymph node and Peyer's patch can induce both production of IL-4 and IFN-γ of naive CD4^+ T cells in their primary culture, depending on antigen doses.

来自脾脏、肠系膜淋巴结和派尔氏集结的树突状细胞可以诱导原代培养物中幼稚 CD4^+ T 细胞产生 IL-4 和 IFN-γ，具体取决于抗原剂量。

DOI：
发表时间：
2003
期刊：
Cytotechnology 43
影响因子：
0
作者：
Masanobu Hibi;Satoshi Hachimura;Wataru Ise;Ayuko Sato;Tadashi Yoshida;Tsuyoshi Takayama;Kastumi Sasaki;Takashi Senga;Shuichi Hashizume;Mamoru Totsuka;Shuichi Kaminogawa.
通讯作者：
Shuichi Kaminogawa.

新しい診断と治療のABC 26/免疫 4 食物アレルギー

新诊疗基础知识26/免疫学4 食物过敏

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
Masanobu Hibi;Satoshi Hachimura;Wataru Ise;Ayuko Sato;Tadashi Yoshida;Tsuyoshi Takayama;Kastumi Sasaki;Takashi Senga;Shuichi Hashizume;Mamoru Totsuka;Shuichi Kaminogawa.;Mamoru Totsuka. 戸塚護;戸塚護(分担執筆)
通讯作者：
戸塚護(分担執筆)

Altered gene expression in intestinal intraepithelial lymphocytes derived from mice fed a specific antigen. (in Japanese : 特異抗原経口投与時における小腸上皮内リンパ球の遺伝子発現変化)

来自饲喂特定抗原的小鼠的肠上皮内淋巴细胞的基因表达发生改变。