Studies on biochemical properties of fish skin-mucus glycoproteins

鱼皮粘液糖蛋白生化特性的研究

• 批准号: 15580181
• 负责人: HAMA Yoichiro
• 金额: $ 1.66万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580181/
• 关键词: fish; skin mucus; glycoprotein; mucin; N-linked; O-linked; 粘質物

This research was conducted to clarify the characteristics of fish skin-mucus glycoproteins. Five kinds of fish species that are indigenous to Ariake Sea, Mutsugoro, Warasubo, Hazekuchi, Akashitabirame, and Shoukihaze, were used for fish samples.Fish skin-mucus was prepared from live fish samples. Glycoproteins in the skin-mucus were separated by Nuclease digestion, heat treatment, acid treatment, ion-exchange chromatography, and gel chromatography. Chromatographic behaviors on both ion-exchange and gel chromatographies of glycoproteins from Akashitabirame and Shoukihaze (the group I) was almost identical to those of glycoproteins from other fish species we had already studied so far. However, glycoproteins from Mutsugoro, Warasubo, and Hazekuchi (the group II) did not behaved similarly in both chromatographies.Sugar compositional analysis of these glycoproteins revealed that : (1)total sugar contents of glycoproteins from the groups I and II were 50-75% and around 10%, respectively : (2)NeuAc and GalNAc were found dominantly in the group I, and GlcNAc,Gal,Man,NeuAc were found in the group II. Mannose is characteristic for N-linked glycoproteins but not for O-linked glycoproteins. Mannose had not been detected in glycoproteins isolated from fish mucus.Sugar chains of the glycoproteins could be released by β-elimination and hydrazinolysis for the groups I and II, respectively. Therefore, glycoproteins from the groups I and II were confirmed to be O-linked and N-linked glycoproteins, respectively. Structure of major sugar chain from the group I was identical, and was identified to be NeuAcα2-6GalNAc. On other hand, structure of the major sugar chain from Mutsugoro (the group II) was presumed to be the complex type, which was composed of NeuAc,Gal,Man,and GlcNAc.

本研究的目的是阐明鱼皮粘液糖蛋白的特性。 Five kinds of fish species that are indigenous to Ariake Sea, Mutsugoro, Warasubo, Hazekuchi, Akashitabirame, and Shoukihaze, were used for fish samples.Fish skin-mucus was prepared from live fish samples.通过核酸酶消化、热处理、酸处理、离子交换色谱和凝胶色谱分离皮肤粘液中的糖蛋白。 Akashitabirame 和 Shoukihaze（第一组）糖蛋白在离子交换和凝胶色谱上的色谱行为与我们迄今为止研究过的其他鱼类糖蛋白的色谱行为几乎相同。 However, glycoproteins from Mutsugoro, Warasubo, and Hazekuchi (the group II) did not behaved similarly in both chromatographies.Sugar compositional analysis of these glycoproteins revealed that : (1)total sugar contents of glycoproteins from the groups I and II were 50-75% and around 10%, respectively : (2)NeuAc and GalNAc were found dominantly in the group I, and GlcNAc,Gal,Man,NeuAc were found in the group II.甘露糖是 N 连接糖蛋白的特征，但不是 O 连接糖蛋白的特征。从鱼粘液中分离的糖蛋白中未检测到甘露糖。I组和II组的糖蛋白分别通过β-消除和肼解作用释放糖链。因此，I组和II组的糖蛋白分别被确认为O-连接糖蛋白和N-连接糖蛋白。 I组的主糖链结构相同，被鉴定为NeuAcα2-6GalNAc。 On other hand, structure of the major sugar chain from Mutsugoro (the group II) was presumed to be the complex type, which was composed of NeuAc,Gal,Man,and GlcNAc.