Screening for the novel glycanase using fish mucus glycoprotein

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O-Glycanase is an enzyme which is capable of releasing entire sugar chains from O-glycan such as mucin. However, since the enzymes which were reported to data act only specific structures, those which could liberate sugar chains from O-glycans more universally are required. In this study, we isolated O-glycan from fish mucus with novel sugar chain structures, and used the O-glycan as the substrate for screening the O-glycanase-producing bacterium. We tested over 500 samples such as soil, environmental water, and fish mucus, but we could not detect the activity of the target enzyme. However, release of KDN at the non-reduing end of the glycan observed very often. KDN-containing O-glycan in the fish mucus is thought to be a protective barrier from the environmental microbes.