Screening for the novel glycanase using fish mucus glycoprotein

利用鱼粘液糖蛋白筛选新型聚糖酶

• 批准号: 23580281
• 负责人: HAMA Yoichiro
• 金额: $ 3.41万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580281/
• 关键词: O-グリカナーゼ; 糖質分解酵素; 粘質物; 魚類体表; 魚類; ムチン; O-グリカン

O-Glycanase is an enzyme which is capable of releasing entire sugar chains from O-glycan such as mucin. However, since the enzymes which were reported to data act only specific structures, those which could liberate sugar chains from O-glycans more universally are required. In this study, we isolated O-glycan from fish mucus with novel sugar chain structures, and used the O-glycan as the substrate for screening the O-glycanase-producing bacterium. We tested over 500 samples such as soil, environmental water, and fish mucus, but we could not detect the activity of the target enzyme. However, release of KDN at the non-reduing end of the glycan observed very often. KDN-containing O-glycan in the fish mucus is thought to be a protective barrier from the environmental microbes.

o -聚糖酶是一种能够从o -聚糖（如粘蛋白）中释放整个糖链的酶。然而，由于已报道的酶仅作用于特定结构，因此需要能够更普遍地从o -聚糖中释放糖链的酶。本研究从鱼黏液中分离出具有新型糖链结构的o -聚糖，并将其作为底物筛选o -聚糖酶产菌。我们测试了500多个样品，如土壤、环境水和鱼粘液，但我们无法检测到目标酶的活性。然而，在聚糖的非还原端经常观察到KDN的释放。鱼类黏液中含有kdn的o -聚糖被认为是一种保护屏障，可以防止环境微生物的侵害。