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Studies on biologically active proteinaceous substances contained in the skin mucus of the Japanese eel

日本鳗鱼皮肤粘液中生物活性蛋白质物质的研究

基本信息

批准号：
02660211
负责人：
SHIOMI Kazuo
金额：
$ 1.34万
依托单位：
Tokyo University of Fisheries
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660211/
关键词：
Japanese eel European eel Pike eel Skin mucus Proteinaceous toxins Lectins Serum toxins Purification

项目摘要

1. Extracts of the skin mucus of the Japanese eel Anguilla japonica were found to be highly lethal to mice when administered intravenously. The toxin was an acidic protein with a molecular weight of 400000 and was comprised of two types of subunits with molecular weights of 230000 and 110000. Gly and Ala were abundant in the toxin while Met, Cys, Tyr and Trp were very poor. The LD_<50> (i. v. injection into mice) of the purified toxin was estimated to be as low as 3.1 mug/kg.2. At least two lectins, both of which were specific to galactose-related sugars, were detected in the skin mucus of A. japonica. One of them was purified and assumed to be an acidic protein with a molecular weight of 40000-50000. Hemolysins were also contained in the skin mucus but their purification was unsuccessful due to instability.3. A proteinaceous toxin was purified from the serum of A. japonica. The purified toxin had an i. v. LD_<50> of 670 mug/kg and a molecular weight of 150000. Since no neutralization of the serum toxin was exhibited by the antiserum raised against the skin toxin of A. japonica, both toxins were immunologically distinguishable from each other.4. The presence of toxins in the skin mucus was newly established in the following fish : European eel Anguilla anguilla, pike eel Muraenesox cinereus, conger eel Conger myriaster, loach Misgurnus anguillicaudatus, sculpin Cottus pollux and two species of puffer, Takifugu niphobles and Canthigaster rivulata. The toxins of A. anguilla and M. cinereus were chemically and immunologically similar to that of A. japonica.

1.研究发现，日本鳗鱼（Anguilla japonica）皮肤粘液的提取物经静脉注射后对小鼠具有高度致死性。该毒素是一种分子量为40万的酸性蛋白质，由分子量为23万和11万的两种亚基组成。毒素中甘氨酸和丙氨酸含量较高，蛋氨酸、半胱氨酸、酪氨酸和色氨酸含量较低。LD_（i.<50>静脉注射到小鼠中）的纯化毒素估计低至3.1 μ g/kg。在A.的皮肤粘液中至少检测到两种凝集素，这两种凝集素都是半乳糖相关糖特异性的。日本。其中一个被纯化，并假定是一个酸性蛋白，分子量为40000 - 50000。皮肤粘液中也含有溶血素，但由于不稳定，纯化不成功.从A.日本。纯化后的毒素具有I. v.LD_670 μ g/kg和分子量150000。<50>由于抗A. japonica，两种毒素在免疫学上是可以区分的.在以下鱼类的皮肤粘液中发现了毒素：欧洲鳗Anguilla anguilla、海鳗Muraenesox cinereus、海鳗Conger myriaster、泥鳅Misgurnus anguillicaudatus、杜父鱼Cottus pollux和两种河豚鱼Takifugu niphobles和Canthigaster rivulata。A. anguilla和M. cinereus与A.日本。