权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Evaluation of endocrine disruptors using chicken fertilized egg

利用鸡受精卵评价内分泌干扰物

基本信息

批准号：
15580249
负责人：
MAEDA Teruo
金额：
$ 2.24万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Various doses of diethylstilbestrol(DES), nonylphenol(NP) and bisphenol A(BPA) were injected into chicken fertilized eggs, and the influences on the viability on day 7 of incubation, hatchability and development of the gonads were examined. Both the viability and hatchability were significantly lower, and a sex-reverse male (phenotypic female having ovary like left gonad regardless of genotypic male) appeared when high dose of DES (1mg) was injected to the eggs. Though high doses of NP or BPA (100mg) was also poisonous for the development of chicken embryos, they had no effect on the male left testis into the ovary. Exposure to high dose of DES, NP and BPA appear to be harmful to the development of embryos in wildlife. The effects of BPA and o,p'- DDT on mRNA expression of vitellogenin (VTG II) in the liver of quail embryo after their sexing were also investigated. RT-PCR analysis detected no mRNA expression of VTG II in the liver of males and females in the control group, whereas distinct VTG II mRNA transcripts were detected in the Estradiol 17β treated female and male groups (183 bp). In BPA treated group, there was a slight expression of VTG II in female embryos. However, no induction of mRNA expression was detected in male embryos. o,p'- DDT treatment induced a remarkable expression of VTG II in female embryos. Moreover, it induced a slight increase in the mRNA levels of VTG II in male embryos at the dose used. Both chemicals have weak estrogenic action.In conclusion, chicken fertilized eggs can be utilized to assess endocrine disruptors, which have estrogenic activity. BPA and o,p'-DDT have weak estrogenic action.

将不同剂量的己烯雌酚（DES）、壬基酚（NP）和双酚A（BPA）注射到鸡受精卵中，考察其对孵化第7天活力、孵化率和性腺发育的影响。当向卵中注射高剂量DES（1mg）时，存活率和孵化率均显着降低，并且出现性别反转雄性（无论基因型如何，雌性都具有类似左侧性腺的表型雄性）。虽然高剂量的NP或BPA（100毫克）对鸡胚胎的发育也有毒，但它们对雄性左睾丸进入卵巢没有影响。接触高剂量的 DES、NP 和 BPA 似乎对野生动物的胚胎发育有害。还研究了 BPA 和 o,p'- DDT 对雌雄鉴定后鹌鹑胚胎肝脏中卵黄蛋白原 (VTG II) mRNA 表达的影响。 RT-PCR 分析检测到对照组男性和女性肝脏中没有 VTG II mRNA 表达，而雌二醇 17β 处理的女性和男性组中检测到不同的 VTG II mRNA 转录本（183 bp）。在BPA处理组中，VTG II在雌性胚胎中有轻微表达。然而，在雄性胚胎中没有检测到 mRNA 表达的诱导。 o,p'- DDT 处理诱导雌性胚胎中 VTG II 的显着表达。此外，在所用剂量下，它还诱导雄性胚胎中 VTG II mRNA 水平略有增加。这两种化学物质都具有微弱的雌激素作用。总之，鸡受精卵可用于评估具有雌激素活性的内分泌干扰物。 BPA 和 o,p'-DDT 具有弱雌激素作用。