Potential of chicken egg (IgY) neutraceutical antibodies to improve animal production and food safety through passive immune protection against pathogens

近年来，多种抗生素耐药细菌的出现以及新抗生素的有限开发和批准，给降低风险和限制对农业和食品安全行业以及公共卫生至关重要的细菌病原体的传播带来了严峻而艰巨的挑战。通过给药目标特异性抗体的被动免疫可以为减少细菌病原体提供一种有希望和可行的替代抗生素。这种抗体的施用可以提供一种防止细菌定植的手段，以及一种低耐药性的快速保护。有效的保护可能需要反复给药，因为这种抗体在肠道或循环系统中存在有限的时间。这项合作研究与发展资助涉及免疫生物解决方案、魁北克鸡蛋生产者联合会和INRS的一个学术研究小组。他们的目标是证明从鸡蛋中提取的抗体对猪和人类的一种重要细菌病原体艰难梭菌的功效。抗体提取的程序是由Immune Biosolutions开发的，现在需要进行调整，以提供更工业化规模的提取和加工。该研究小组将由inrs - center Armand-Frappier教授C. M. Dozois领导，他将协调该项目，并将负责在细胞培养和动物挑战模型中证明抗艰难梭菌毒素抗体的生物学功效。这项研究将建立抗体生产/提取的新方法，并证明预防/治疗艰难梭菌感染的可行性，但重要的是，也确立了该技术在减少感染或防止与农业、水产养殖、食品安全和公共卫生相关的其他特定病原体定植方面的潜力。

The emergence of multiple antibiotic resistant bacteria and the limited development and approval of new antibiotics in recent years have resulted in a critical and daunting challenge to reduce the risk and limit the spread of bacterial pathogens of importance to agricultural and food safety industries, and public health. Passive immunity through administration of target-specific antibodies can provide a promising and feasible alternative to antibiotics for reduction of bacterial pathogens. Administration of such antibodies can provide a means of preventing bacterial colonization as well as a rapid protection with a low probability of resistance. Effective protection can require repeated administration as such antibodies are present for a limited period in the intestinal tract or circulatory system. This Collaborative Research and Development grant involves Immune Biosolutions, the Quebec Federation of Egg Producers, and an academic research team from INRS. Their goal is to demonstrate the efficacy of antibodies extracted from chicken eggs against an important bacterial pathogen for both swine and humans, Clostridium difficile. The procedure for antiboby extraction has been developed by Immune Biosolutions, and now requires adaptations to provide extraction and processing at a more industrial scale. The research team will be led by C. M. Dozois, Prof. at INRS-Centre Armand-Frappier, who will coordinate the project and will be responsible for demonstrating the biological efficacy of the antibodies against C. difficile toxins in cell culture, and in animal challenge models. This research will establish new methods for production/extraction of antibodies and demonstrate the feasibility of prevention/treatment of C. difficile infections, but importantly also establish the potential for this technology to reduce infections or prevent colonization of other specific pathogens of relevance to agriculture, aquaculture, food safety and public health.