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Evolution and host adaptation strategy of carnivorous parvoviruses

肉食性细小病毒的进化和宿主适应策略

基本信息

批准号：
15580255
负责人：
TOHYA Yukinobu
金额：
$ 2.37万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580255/
关键词：
dog cat parvovirus host range evolution mutation monoclonal antibody virulence 宿主城

项目摘要

The final goal of this study is to define evolution (mutation) of carnivorous parvoviruses and to analyze mechanisms lying for the viruses to gain ability to infect and replicate in new host species. During the study, we obtained several new findings as follows.1)Sequence analysis on the nonstructural protein gene was performed using a new antigenic variant V203 strain from a Vietnamese cat and antigenic type CPV2b strains isolated from Vietnamese cats and Japanese dogs. Compared with Japanese strains, all the Vietnamese strains had a unique substitution (Glu to Val) at the amino acid position 545.2)Four new monoclonal antibodies (mAbs) were produced against V203 strain. These mAbs were categorized into three groups according to the reactivity to each of the antigenic type strain. Results of the analysis using resistant variants showed that two mAbs recognized the antigenic site A and that the other two recognized the antigenic site B or its neighborhood.3)Out of the 126 rectal swab samples obtained from dogs and cats in Hanoi and Ho Chi Meth cities in Vietnam, eight parvovinus strains were isolated from dogs and one from a cat.4)The new Vietnamese isolates were examined on their antigenic types using the mAbs. Eight strains including one from a cat were clearly shown to be CPV-2b, but HM-4-1 strain isolated from a dog in Hanoi city showed much lower reactivity to two of the mAbs.5)HNI-4-1 strain had an amino acid substitution at a position 426 in VP2, suggesting that HNI-4-1 strain was a novel antigenic variant of CPV-2b.In conclusion, the mAbs produced in this study are expected to be useful for the detection as well as typing of parvoviruses including newly emerging antigenic variants, because carnivorous parvoviruses are still considered to be evolving by mutations.

本研究的最终目标是定义食肉细小病毒的进化（突变），并分析病毒获得感染和复制新宿主物种的能力的机制。本研究取得了以下新的发现：1）对越南猫新抗原变异株V203和越南猫、日本犬CPV 2b株的非结构蛋白基因进行了序列分析。与日本株相比，所有越南株在545位氨基酸上都有一个独特的替换（Glu替换为瓦尔）。2）制备了4株新的抗V203株单克隆抗体。根据对每种抗原型菌株的反应性，将这些mAb分为三组。使用抗性变体的分析结果表明，两种mAb识别抗原位点A，另外两种识别抗原位点B或其邻近位点。3）在越南河内和胡志明市的126个狗和猫的直肠拭子样品中，从狗中分离出8个细小病毒株，从猫中分离出1个细小病毒株。4）使用单克隆抗体检测新的越南分离株的抗原类型。其中8株为CPV-2b，其中1株来自猫，但从河内市犬分离的HM-4-1株与其中2株的反应性较低。5）HNI-4-1株在VP 2的第426位氨基酸发生了突变，提示HNI-4-1株是CPV-2b的一种新的抗原变异株。由于食肉性细小病毒仍然被认为是通过突变而进化的，因此预期本研究中产生的mAb可用于细小病毒（包括新出现的抗原变体）的检测和分型。