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Studies on molecular determinants for virulence of lethal infection of infectious bursal disease virus

传染性法氏囊病病毒致死感染毒力的分子决定因素研究

基本信息

批准号：
15580257
负责人：
YAMAGUCHI Tsuyoshi
金额：
$ 2.37万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580257/
关键词：
IBDV infectious bursal disease virulence lethality 鶏 VP5 VP3 相互作用 VP2

项目摘要

Infectious bursal disease virus (IBDV) is the causative agent of immunosuppressive disease of young chicken. There are two types of IBDV based on the lethality for chicken, highly virulent and classical types. However, the molecular determinants for the difference of lethality have not been defined. In the present study, IBDV strains which show high mortality and low mortality designated as highly virulent type and classical type, respectively were used for comparative study of subcellular localization of viral structural proteins, VP2,VP3 and VP4, and nonstructureal protein VP5. In addition, cell toxicity of each viral protein was analyzed. For the VP2 and VP5, bacterial 2-hybrid system was used to determine the cellular molecules which interact with the viral proteins. Comparative study of subcellular localization of each viral protein showed that the VP5 showed differences between the highly virulent type and the classical type. Furthermore, VP5 derived from highly virulent strain showed higher cell toxixity than that of VP5 derived from classical type. Unfortunately, the cellular molecules which interact with the viral proteins were not detected. These results indicate that VP5 is responsible for the virulence of highly virulent IBDV. Previous report of the deduced amino acid sequence allignment showed 4 amino acid differences between the sequence of highly virulent type and classical type. The only 4 amino acids in VP5 may contribute to the virulence of highly virulent type of IBDV.

传染性法氏囊病病毒（IBDV）是雏鸡免疫抑制病的病原。根据对鸡的致死率，有两种类型的IBDV，高毒力型和经典型。然而，造成致死率差异的分子决定因素尚未确定。本研究采用高致死率和低致死率分别为高毒型和经典型的IBDV菌株，对病毒结构蛋白VP2、VP3和VP4以及非结构蛋白VP5的亚细胞定位进行了比较研究。此外，还分析了各病毒蛋白的细胞毒性。对于VP2和VP5，采用细菌2-杂交系统测定与病毒蛋白相互作用的细胞分子。对各病毒蛋白亚细胞定位的比较研究表明，VP5在高毒型和经典型之间存在差异。此外，高毒力菌株衍生的VP5比经典型衍生的VP5具有更高的细胞毒性。不幸的是，没有检测到与病毒蛋白相互作用的细胞分子。这些结果表明VP5与高毒力IBDV的毒力有关。先前报道的氨基酸序列比对结果显示，高毒型与经典型之间存在4个氨基酸的差异。VP5中仅有的4个氨基酸可能与高毒型IBDV的毒力有关。