Molecular biological studies on virulence factor s and molecular pathogenesis of infectious bursal dissease virus

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In the present study, to understand the molecular mechanism for the virulence or pathogenicity of the virulent infectious bursal disease virus (IBDV), several experiments were employed as listed below.1. Usefulness of chicken B lymphoma LSCC-DT40 cell as a tool for in vitro analysis of virulent IBDV was examined. Although freshly isolated virulent IBDV doesn't propagate in chicken embryo fibroblast cell without adaptation, the virulent IBDV easily propagate in DT40 cell. This findings indicate the usefulness of the cell for in vitro analysis of the virulent IBDV.2. Cytotoxicity and subcellular localization of viral proteins of virulent IBDV was examined. Transient expression of VP4 derived from virulent IBDV localized in nucleus and is apoptotic to the host cell. Furthermore, it was revealed that palmitoylation of VP5 is important for the localization of VP5 at plasma membrane, which is known as essential event for cell lysis observed in IBDV infection.3. Microarray analysis of host cell gene transcription in response to virulent IBDV infection of DT40 was examimned. Transcriptional change in 170 genes of DT40 cell was found after virulent IBDV infection. Different transcriptional changes were found depend on the virulence of IBDV.4. IBDV infection resistant DT40 cell was established and the transcriptional profiles were examined. Thousands of significant changes were found in the transcription between IBDV susceptible and resistant DT40 cells.