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Detection and treatment of repeat breeder cows with altered epidermal growth factor expression in the uterus

子宫内表皮生长因子表达改变的重复种牛的检测和治疗

基本信息

批准号：
15580284
负责人：
KATAGIRI Seiji
金额：
$ 2.37万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

This project aimed to develop diagnostic method and treatment of repeat breeder cows with uterine dysfunction as a cause of infertility. First the profile of epidermal growth factor (EGF) in the endometrium was determined. EGF showed two peaks during the estrus cycles, Days 2-4 and 13-14. About 70% of repeat breeder cows showed abnormality in the EGF profile, typically lack of these peaks. This abnormality persisted between the estrous cycles and normalization of EGF profile resulted in restoration of fertility, indicating that the abnormal EGF profile may be a part of causes of infertility in dairy cattle. Then, treatment to the abnormality has been developed. When a high dose of estradiol-benzoate (EB, 5 mg) was given in combination of a CIDR device, the EGF profile was normalized in about 70% and 70-80% of them became pregnant after treatment. Then, the EGF profile was determined in recipient cattle for embryo transfer (ET) to assess if the endometrial EGF profile may serve as an indicator of fertility in cattle. Cows with a normal EGF profile showed higher pregnancy rate after ET compared with those with an abnormal EGF profile (70 vs. 30%), indicating the EGF profile can be an indicator of fertility and, thus, become a useful marker of recipient selection in ET program. Finally, factors which may affect the recovery of the EGF profile after parturition was examined. Luteal phase of at least for 8 days may be required for the recovery of cyclicity in the endometrial EGF profile. However, appearance of a luteal phase (> 11 days) before uterine involution and repeated short (< 8 days) luteal phases seemed a risk factor to increase the frequency of a abnormal EGF profile at the time of insemination.

本项目旨在探讨以子宫功能障碍为不育原因的重复种牛的诊断方法和治疗方法。首先确定了子宫内膜中表皮生长因子（EGF）的分布。EGF在发情周期第2 ~ 4天和第13 ~ 14天出现两个高峰。约70%的重复种牛EGF谱显示异常，通常缺乏这些峰值。这种异常持续存在于发情周期和EGF谱的正常化之间，导致奶牛的生育能力恢复，这表明EGF谱的异常可能是奶牛不育的部分原因。然后，对异常的治疗已经发展起来。当高剂量雌二醇-苯甲酸酯（EB, 5mg）与CIDR装置联合使用时，约70%的EGF谱恢复正常，70-80%的人在治疗后怀孕。然后，在胚胎移植（ET）受体牛中测定EGF谱，以评估子宫内膜EGF谱是否可以作为牛生育能力的指标。EGF表达正常的奶牛在ET后的妊娠率高于EGF表达异常的奶牛（70% vs. 30%），这表明EGF表达可以作为生育能力的指标，从而成为ET计划中受体选择的有用标记。最后，探讨了可能影响分娩后EGF谱恢复的因素。至少8天的黄体期可能需要恢复子宫内膜EGF的周期性。然而，在子宫复旧前出现黄体期（10 ~ 11天）和反复出现的短黄体期（< 8天）似乎是人工授精时增加异常EGF谱频率的危险因素。