Immunohistochemical detection of cell components related to the acid secreting dynamics in gastric parietal cells.

本研究的主要目的是使细胞在哪里或不是在功能上类似于通过地面，以及细胞基元组件的转化机制与锌分子和氯离子通道的分布有关。在本研究中，该物种被处理为一种冷冻方法，在光和电子微观水平上展示了良好的形态保存和保留溶剂组件。为了抑制它们的免疫化学性质的严重冰晶体损伤或损失，从广告Libitum-fed或Starved的老鼠准备好用快速冻结跟随的快速冻结--用于光显微镜替代，以及用于电子显微镜的高压力冻结。“半细胞的可变形结构”被测试了酸保密的活跃和静止阶段之间的关系。这些结构是退化的内衬金丝雀，包括秘密阶段中的数字微囊，以及在apicalcy中开发的管膜。 ... More toplasm at resting phase.用H^+·K^+-ATP酶抗体在两个相位的细胞中本地化。标签比带有化学固定的室内金丝雀、微型细菌和管子的薄膜更密集和清晰。“积极的固定的半导体细胞被分散，大幅度地穿过地球。”细胞被秘密地定位在上面的第三个地球上，被严重地固定在一个反磷酸化的异黄酮抗体上。在静止的阶段，抗体表现出了一种微弱的反应。However， the anti-nonphosphorylated ezrin antibody hardly labeled those cells。反氯离子通道抗体免疫性抑制在保密阶段展示了颈部区域周围积极反应的狭窄区域。这些标签被发现在室内金丝雀、微型细菌和结核杆菌的薄膜上。它被包括在上面的第三个细胞中，有更多的活性细胞用于功能性封闭比其他部分更活跃的细胞，而半导体细胞则分散出了垃圾地面。Less（低）

The main purpose of this study was to ascertain whether or not the cells are functionally similar throughout the gland, and how transformation mechanism with cytoskeletal components is related to the distribution of ezrin molecules and chloride channel. In this study, the specimens were processed with cryofixation method showed good morphological preservation and retention of soluble components at the light and electron microscopic level. To suppress severe ice-crystal damage or loss of their immunocytochemical properties, stomach tissues from ad libitum-fed or starved rats were prepared with rapid freezing followed by freeze-substitution for light microscopy, and with high pressure freezing for electron microscopy. The transformable structure of parietal cell was examined between the active and resting phases of acid secretion. These structures were the dilated intracellular canaliculi, including numerous microvilli in the secreting phase, and the developed tubulovesicles in apical cy … More toplasm at resting phase. The immunohistochemical staining with anti-alpha- or beta-subunits of H^+・K^+-ATPase antibodies was localized on the cells from both phases. Labeling was more intense and clear on the membranes of intracellular canaliculi, microvilli and tubulovesicles than that with chemical fixation. The positively stained parietal cells were scattered widely throughout the gland. The cells in the secreting phase located in the upper one third of the gland were densely stained with an anti-phosphorylated ezrin antibody. In the resting phase, the antibody showed a weak reaction. However, the anti-nonphosphorylated ezrin antibody hardly labeled those cells. Anti-chloride channel antibody immunostaining showed a narrow zone of positive reaction around the neck region in the secreting phase. These labelings were observed on the membrane of intracellular canaliculi, microvilli and tubulovesicles. It was concluded that the cells in around the upper one third are more active cells for functional secretion than those of other segments, while parietal cells were distributed throughout the gastric gland. Less