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Immunohistochemical analyses of occludin expression in human diseases and their application for diagnostic pathology

人类疾病中occludin表达的免疫组织化学分析及其在诊断病理学中的应用

基本信息

批准号：
15590306
负责人：
TOBIOKA Hirotoshi
金额：
$ 2.24万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590306/
关键词：
tight junction neoplasm occludin immunohistochemistry carcinoma 不妊症

项目摘要

The tight junction is one of the most characteristic structural markers of the polarized epithelial phenotype. We established a new anti-occludin monoclonal antibody that can be used for formalin-fixed paraffin- embedded tissue sections. And we examined the usefulness of this antibody for the diagnostic surgical pathology of human diseases.By using this antibody, we examined the expression of occludin in 68 human lung carcinomas and surrounding normal lung tissues. In normal lung tissues, the anti-occludin antibody strongly stained the apicoluminal borders of the bronchiaUbronchiolar epithelia and bronchial glands as a dot or short line. The antibody also stained the intercellular borders of alveolar epithelia. In cancer cells that faced lumina of all adenocarcinomas, regardless of grade, including bronchioloalveolar carcinomas, occludin showed an expression pattern identical to that of the normal bronchial and alveolar epithelia. Occludin reactivity was not noted in any cases of squamous cell carcinoma, large cell carcinoma, small cell carcinoma, or large cell neuroendocrine carcinoma. The results of the present study suggest that occludin can serve as an immunohistochemical indicator of the "true" glandular differentiation that forms tubulo-papillary structures in human lung carcinoma tissues.Then we examined ooccludin expression in human breast invasive ductal carcinomas and lobular carcinomas. Occludin expression was detected in all cases of invasive ductal carcinomas. However, occludin was detected in only 20% of invasive lobular carcinomas. We concluded that occludin immunostaing could be a useful marker for distinguishing these two types of invasive breast carcinomas.We also examined 40 human rectal carcinoid tumors. In 8 (20%) samples of typical carcinoid tumors, a small number of resette-like tubular structures outlined by occludin were detected. The results provide supportive evidence that carcinoid tumor cells are capable of glandular differentiation.

紧密连接是极化上皮表型最具特征性的结构标志之一。我们建立了一种新的抗闭合蛋白单克隆抗体，可用于福尔马林固定石蜡包埋的组织切片。利用该抗体检测了68例肺癌组织和癌旁正常肺组织中occludin的表达。在正常肺组织中，抗occludin抗体强烈染色支气管上皮细胞和支气管腺体的顶腔边界，呈点状或短线。该抗体还染色肺泡上皮细胞间的边界。在所有腺癌（无论级别如何，包括细支气管肺泡癌）的癌细胞中，occludin的表达模式与正常支气管和肺泡上皮细胞相同。在鳞状细胞癌、大细胞癌、小细胞癌或大细胞神经内分泌癌的任何病例中均未观察到Occludin反应性。本研究的结果表明，occludin可以作为一个免疫组化指标的“真正的”腺分化，形成管状乳头状结构在人肺癌组织中。然后，我们研究了occludin在人乳腺浸润性导管癌和小叶癌的表达。在所有浸润性导管癌中均检测到Occludin表达。然而，occludin仅在20%的浸润性小叶癌中检测到。我们的结论是，occludin免疫染色可能是区分这两种类型的浸润性乳腺癌的一个有用的标记。在8例（20%）典型类癌中，检测到少量由occludin勾画的重置样管状结构。这些结果为类癌细胞具有腺分化能力提供了支持性证据。

项目成果

期刊论文数量（40）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Tight junctions and human diseases.

DOI：
10.1007/s00795-003-0219-y
发表时间：
2003-09-01
期刊：
Medical Electron Microscopy
影响因子：
0
作者：
Sawada, Norimasa;Murata, Masaki;Chiba, Hideki
通讯作者：
Chiba, Hideki

Expression of occludin in human rectal carcinoid tumors as a possible maker of glandular differentiation

occludin 在人直肠类癌中的表达作为腺体分化的可能标志

DOI：
发表时间：
2004
期刊：
Histopathology 44
影响因子：
0
作者：
Shinmura;K;Kuroda;M;et al.;Tokunaga Y.
通讯作者：
Tokunaga Y.

Expression of occludin in human rectal carcinoid tumors as a possible marker of glandular differentiation.

occludin 在人直肠类癌中的表达作为腺体分化的可能标志。

DOI：
发表时间：
2004
期刊：
Histopathology. 44
影响因子：
0
作者：
Yoshida S;Nakamura N;Sasaki Y;Yoshida S;Yasuda M;Sagara H;Ohtake T;Takenoshita S;Abe M.;Tokunaga Y et al.
通讯作者：
Tokunaga Y et al.

Occludin expression disease with the progression of human endooometrial carcinoma.

Occludin 表达疾病与人子宫内膜癌的进展。