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Functional analyses of esophageal glands of parasitic nematodes

寄生线虫食管腺的功能分析

基本信息

批准号：
15590372
负责人：
MARUYAMA Haruhiko
金额：
$ 2.3万
依托单位：
NAGOYA CITY UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1.Changes in Strongyloides venezuelensis larvae upon entry into the hostInfective larvae of S.venezuelensis has a matrix metalloprotease. We found that this activity disappeared right after the entry into the host, which was associated with the disappearance of skin penetration ability. In addition to the percutaneous infectivity and metalloprotease activity, drastic changes in locomotion occurred in larvae. In order to identify factors that caused these changes in larvae, we cultured infective larvae in serum-free culture medium at 37 degrees for 3 days. We clearly demonstrated that infective larvae transformed into connective tissue larvae as judged by all parameters we examined. Trans formation required both temperature and nutritional factors.2.Secreted adhesion molecules of adult wormsAdult worms of S.venezuelensis have a potent mucoal invasion capacity, which was confirmed by surgically implanted adult worms invading into chick small intestine. During tissue migration, larvae started expressing adhesion molecules, which are essentially important in mucosal invasion in the lungs.3.Effets of secreted antigens on host immune systemS.venezuelensis is usually expelled from the host by mucosal mast cells which are T cell-dependent. However, in IL-5 transgenic mice, hyperactivated eosinophils infiltrated into epithelial layer of small intestine and cleared mucosal adult worms. Studies on T cell induction in S. venezuelensis infected mice revealed that collaboration between IL-18-dependent mastocytosis before antigenic activation of T cells, and Th2 cell-dependent mastocytosis is important for prompt parasite expulsion.

1.委内瑞拉圆线虫幼虫进入宿主后的变化委内瑞拉圆线虫感染性幼虫具有基质金属蛋白酶。我们发现这种活性在进入宿主后立即消失，这与皮肤渗透能力的消失有关。除了经皮感染性和金属蛋白酶活性外，幼虫的运动也发生了剧烈变化。为了确定引起幼虫这些变化的因素，我们将感染性幼虫在无血清培养基中37度培养3天。我们清楚地证明，根据我们检查的所有参数判断，感染性幼虫转化为结缔组织幼虫。转化需要温度和营养因素的共同作用。 2.成虫分泌的粘附分子委内瑞拉链球菌成虫具有强大的粘膜侵袭能力，通过手术植入成虫侵入雏鸡小肠证实了这一点。在组织迁移过程中，幼虫开始表达粘附分子，这对于肺部粘膜侵袭至关重要。3.分泌抗原对宿主免疫系统的影响委内瑞拉链球菌通常通过T细胞依赖性粘膜肥大细胞从宿主体内排出。然而，在IL-5转基因小鼠中，过度活化的嗜酸性粒细胞浸润到小肠上皮层并清除粘膜成虫。对委内瑞拉沙门氏菌感染小鼠中 T 细胞诱导的研究表明，T 细胞抗原激活前的 IL-18 依赖性肥大细胞作用和 Th2 细胞依赖性肥大细胞作用之间的协作对于迅速驱除寄生虫至关重要。

项目成果

期刊论文数量（36）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

家庭医学大全科:線虫によっておきる病気(寄生虫・原虫で起こる感染症)

家庭医学百科全书：线虫引起的疾病（寄生虫、原虫引起的传染病）

DOI：
发表时间：
2004
期刊：
影响因子：
0
作者：
丸山治彦
通讯作者：
丸山治彦

Adult worms of the rodent intestinal nematode, Strongyloides venezuelensis, successfully invade chick intestinal mucosa.

啮齿动物肠道线虫委内瑞拉圆线虫成虫成功侵入雏鸡肠粘膜。

DOI：
发表时间：
2003
期刊：
Parasitology International 52(1)
影响因子：
0
作者：
Maruyama H;Aoki M;Okamura S;Yoshida A;Itagaki T;Ohta N
通讯作者：
Ohta N

ntraepithelial infiltration of eosinophils and their contribution to the elimination of adult intestinal nematode, Strongyloides venezuelensis in mice.

嗜酸性粒细胞上皮内浸润及其对消除小鼠成虫肠道线虫委内瑞拉类圆线虫的贡献。

DOI：
发表时间：
2004
期刊：
Parasitology International 52
影响因子：
0
作者：
Mohamed El-Malky;Haruhiko Maruyama;Yoshiaki Hirabayashi;Shoichi Shimada;Ayako Yoshida;Teruaki Amano;Akira Tominaga;Kiyoshi Takatsu;Nobuo Ohta.
通讯作者：
Nobuo Ohta.

分子寄生虫学入門:寄生虫を作る(C.elegans寄生虫化計画)

分子寄生虫学简介：创建寄生虫（线虫寄生项目）