Studies on the infection mechanisms of Strongyloides venezelensis by analyzing genes expressed in infective larvae.

采用常规EST分析和下一代pyrosequencing大规模测序技术，对啮齿动物肠道线虫委内瑞拉圆线虫（Strongyloides venezuela）感染幼虫的基因表达进行了检测。在感染幼虫期特异性表达的基因中，有近一半编码了功能未知的新蛋白，但也检测到一些与虾青蛋白样金属蛋白酶有关的基因。这些锌金属蛋白酶可能解释了感染幼虫在感染后侵入皮肤的原因。astacin样金属蛋白酶似乎形成一个大的基因家族，提示其在寄生虫病中的重要性。

Gene expression of infective larvae of Strongyloides venezuelensis, an intestinal nematode of rodents, was examined by means of conventional EST analysis and a large-scale sequencing with a next generation pyrosequencer. Nearly half of the genes which were expressed specifically in the infective larva stage coded novel proteins of unknown function, though several genes for astacin-like metalloprotease were detected. These zinc-metalloproteases could account for the skin invasion of infective larvae upon infection. Astacin-like metalloproteases appeared to form a large gene family, suggesting its importance in parsitism.