Studies on the infection mechanisms of Strongyloides venezelensis by analyzing genes expressed in infective larvae.

通过分析感染性幼虫表达的基因来研究威尼斯类圆线虫的感染机制。

• 批准号: 21590466
• 负责人: MARUYAMA Haruhiko
• 金额: $ 3万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590466/
• 关键词: 線虫; トランスクリプトーム; 糞線虫; メタロプロテアーゼ; 寄生虫; 感染; 熱帯病; 体内移行; 感染幼虫; 宿主侵入; 遺伝子発現; 遺伝子ノックダウン; ゲノム

Gene expression of infective larvae of Strongyloides venezuelensis, an intestinal nematode of rodents, was examined by means of conventional EST analysis and a large-scale sequencing with a next generation pyrosequencer. Nearly half of the genes which were expressed specifically in the infective larva stage coded novel proteins of unknown function, though several genes for astacin-like metalloprotease were detected. These zinc-metalloproteases could account for the skin invasion of infective larvae upon infection. Astacin-like metalloproteases appeared to form a large gene family, suggesting its importance in parsitism.

采用常规EST分析和下一代焦磷酸测序仪大规模测序的方法，对啮齿类肠道线虫委内瑞拉圆线虫感染幼虫的基因表达进行了研究。近一半的基因在感染幼虫期特异性表达，编码功能未知的新蛋白质，但也检测到了几个astacin样金属蛋白酶基因。这些锌金属蛋白酶可以解释感染性幼虫在感染后的皮肤侵入。Astacin样金属蛋白酶似乎形成一个大的基因家族，表明其在寄生性中的重要性。

项目成果

Notch2 signaling is required for proper mast cell distribution and mucosal immunity in the intestine.

• DOI: 10.1182/blood-2010-07-289611
• 发表时间: 2011-01
• 期刊: Blood
• 影响因子: 20.3
• 作者: M. Sakata-Yanagimoto;Tôru Sakai;Yasuyuki Miyake;Toshiki I. Saito;H. Maruyama;Y. Morishita;Etsuko Nakagami-Yamaguchi;K. Kumano;H. Yagita;M. Fukayama;S. Ogawa;M. Kurokawa;K. Yasutomo;S. Chiba

A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum

通过 EST 分析在猪蛔虫组织迁移幼虫中鉴定出一种新型 C 型凝集素

• DOI: 10.1007/s00436-011-2677-9
• 发表时间: 2012
• 期刊: Parasitology Research
• 影响因子: 2
• 作者: Yoshida A;Nagayasu E;Horii Y;Maruyama H
• 通讯作者: Maruyama H

ベネズエラ糞線虫のゲノム/トランスクリプトーム解析

委内瑞拉类圆线虫的基因组/转录组分析

• 发表时间: 2011
• 作者: Inoue S;Niikura M;Inoue M;Mineo S;Kawakami Y;Uchida A;Ohnishi H;Kamiya S;Watanabe T;Kobayashi F.;長安英治,伊藤武彦,小椋義俊,吉田彩子,林哲也,丸山治彦
• 通讯作者: 長安英治,伊藤武彦,小椋義俊,吉田彩子,林哲也,丸山治彦

Gene expression analysis of lung stage larvae of Ascaris suum, the swine large intestinal roundworm

猪大肠蛔虫肺期幼虫基因表达分析

• 发表时间: 2009
• 作者: Ayako Yoshida;Eiji Nagayasu;Yoichiro Horii;Haruhiko Maruyama
• 通讯作者: Haruhiko Maruyama

Transcripts analysis of infective larvae of an intestinal nematode, Strongyloides venezuelensis

肠道线虫委内瑞拉类圆线虫感染性幼虫的转录本分析

• 发表时间: 2010
• 期刊: Parasitol Int
• 影响因子: 1.9
• 作者: Yoshida A;Nagayasu E;Nishimaki A;Sawaguchi A;Yanagawa S;Maruyama H
• 通讯作者: Maruyama H