Establishment of transgenic mice that recognize bacteria specific antigen and develop spontaneous colitis under specific pathogen free condition.

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In this study, we clarified that intestinal bacteria flora is essential for the development of chronic colitis using murine model of chronic colitis. We in general used adoptive transfer model that normal CD4+CD45RBhigh T cells from spleens are transferred into SCID mice. This model has the strong advadvantages that we can exactly evaluate the pathogenic T cells in clonal level.We are now under investigation to establish bacteria-specidfic antigen recognizing TCR transgenic mouse after the isolation of TCRab genes. Using this model, we believe that we can brake through to understand the exact mechanism of action in terms of antigen-specific analysis. Furthermore, we will find out a new strategy using probiotics for the treatment of inflammatory bowel diseases.