Development of anti-tuberculosis vaccine using cytokine gene-transduced dendritic cells

使用细胞因子基因转导的树突状细胞开发抗结核疫苗

• 批准号: 15590803
• 负责人: SUDA Takafumi
• 金额: $ 2.11万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590803/
• 关键词: Dendritic cell; vaccine; tuberculosis

In the present study, we evaluated antigen 85A (Ag85A)-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A and injected to BALB/c mice intravenously. The DCs vaccine was capable of generating purified protein derivative (PPD)-, and the antigen-specific spleen cell proliferation and IFN-gamma production from the spleen cells. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) exhibiting lytic activity and IFN-gamma production specific to a CTL epitope on Ag85A molecule. This eliciting cellular immunity led to induction of moderate protective immunity against Mycobacterium tuberculosis

在本研究中，我们评估抗原85 A（Ag 85 A）转导的树突状细胞（DCs）疫苗抗结核分枝杆菌。小鼠骨髓来源的DC用分枝杆菌Ag 85 A逆转录病毒转导并静脉注射到BALB/c小鼠。DC疫苗能够产生纯化的蛋白衍生物（PPD），并且能够从脾细胞增殖抗原特异性脾细胞和产生IFN-γ。此外，DC疫苗接种诱导的细胞毒性T淋巴细胞（CTL）表现出对Ag 85 A分子上的CTL表位特异性的裂解活性和IFN-γ产生。这引发细胞免疫导致诱导针对结核分枝杆菌的适度保护性免疫

项目成果

alpha-Galactosylceramide, a ligand of natural killer T cells, inhibits allergic airway inflammation.

• 发表时间: 2005
• 期刊: American journal of respiratory cell and molecular biology
• 影响因子: 6.4
• 作者: H. Matsuda;T. Suda;J. Sato;T. Nagata;Y. Koide;K. Chida;Hirotoshi Nakamura

Nakamura Y, et al.: "Induction of protective immunity to Listeria monocytogenes with dendritic cells retrovirally transduced with a cytotoxic T lymphocyte epitope minigene"Infect Immun. 71. 1748-1754 (2003)

Nakamura Y等人：“用细胞毒性T淋巴细胞表位小基因逆转录病毒转导的树突状细胞诱导对单核细胞增生李斯特氏菌的保护性免疫”感染免疫。

Differences in clinical features and prognosis of interstitial pneumonia between Polymyositis and dermatomyositis

多发性肌炎与皮肌炎间质性肺炎临床特征及预后的差异

• 发表时间: 2005
• 期刊: J Rheumatol 32
• 作者: Fujisawa T;Suda T;et al.
• 通讯作者: et al.

Vegetable, fruit, and cereal intake and risk of idiopathic pulmonary fibrosis in Japan

• DOI: 10.1159/000082465
• 发表时间: 2004-01-01
• 期刊: ANNALS OF NUTRITION AND METABOLISM
• 影响因子: 3.9
• 作者: Miyake, Y;Sasaki, S;Tanaka, H
• 通讯作者: Tanaka, H

The incidence and clinical charactaristics of bronchiolitis obliterans organizing pneumonia syndrome after radiation therapy for breast cancer

乳腺癌放射治疗后闭塞性细支气管炎机化性肺炎综合征的发生率及临床特点

• 发表时间: 2004
• 期刊: Sarcoidosis Vasc Diffuse Lung Dis 21
• 作者: Miwa S;Suds T;et al.
• 通讯作者: et al.