Development of anti-tuberculosis vaccine using cytokine gene-transduced dendritic cells

在本研究中，我们评估了抗原85 A（Ag 85 A）转导的抗结核分支杆菌的树突状细胞（DCs）疫苗。小鼠骨髓来源的DC用分枝杆菌Ag 85 A逆转录病毒转导并静脉注射到BALB/c小鼠。DC疫苗能够产生纯化的蛋白衍生物（PPD），并且能够从脾细胞增殖抗原特异性脾细胞和产生IFN-γ。此外，DC疫苗接种诱导的细胞毒性T淋巴细胞（CTL）表现出对Ag 85 A分子上的CTL表位特异性的裂解活性和IFN-γ产生。这引发细胞免疫导致诱导针对结核分枝杆菌的适度保护性免疫

In the present study, we evaluated antigen 85A (Ag85A)-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A and injected to BALB/c mice intravenously. The DCs vaccine was capable of generating purified protein derivative (PPD)-, and the antigen-specific spleen cell proliferation and IFN-gamma production from the spleen cells. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) exhibiting lytic activity and IFN-gamma production specific to a CTL epitope on Ag85A molecule. This eliciting cellular immunity led to induction of moderate protective immunity against Mycobacterium tuberculosis