Development of anti-tuberculosis vaccine using cytokine gene-transduced dendritic cells

在本研究中，我们评估了抗原85A （Ag85A）转导的树突状细胞（DCs）抗结核分枝杆菌疫苗。用分枝杆菌Ag85A逆转录病毒转导小鼠骨髓源性dc，并静脉注射到BALB/c小鼠。该dc疫苗能够产生纯化的蛋白衍生物(PPD)-，并能从脾脏细胞中产生抗原特异性的脾脏细胞增殖和ifn - γ。此外，DC疫苗诱导细胞毒性t淋巴细胞（CTL）表现出Ag85A分子上特异性CTL表位的裂解活性和ifn - γ产生。这种诱导细胞免疫导致了对结核分枝杆菌的中度保护性免疫的诱导

In the present study, we evaluated antigen 85A (Ag85A)-transduced dendritic cells (DCs) vaccine against Mycobacterium tuberculosis. Murine bone marrow-derived DCs were retrovirally transduced with mycobacterial Ag85A and injected to BALB/c mice intravenously. The DCs vaccine was capable of generating purified protein derivative (PPD)-, and the antigen-specific spleen cell proliferation and IFN-gamma production from the spleen cells. In addition, the DC vaccination induced cytotoxic T-lymphocytes (CTL) exhibiting lytic activity and IFN-gamma production specific to a CTL epitope on Ag85A molecule. This eliciting cellular immunity led to induction of moderate protective immunity against Mycobacterium tuberculosis