NERBL Core 3: Biocontainment Research Support Services

项目概要-核心3 新英格兰地区生物安全实验室（NERBL）成立于2008年，旨在：（1）确保 针对潜在大流行病原体和其他新发感染（PPP/EI）的研究和任务包括： 安全，高效，严格完成;（2）作为学术和工业的区域资源 参与PPP/EI知识和医疗对策活动的合作伙伴;（3）做好准备 由NIH/NIAID及其联邦合作伙伴指导。NERBL的关键功能包括 开发和不断改进BSL 3/ABSL 3独有的专业研究功能， 特别是那些关注对人类有影响的病原体的研究。在塔夫茨，现有的专业知识和知识 连接了几个重点领域，包括细菌学（结核病[Mtb]）、病毒学（流感）和病媒- 病原体相互作用（东部马和Powassan脑炎，兔热病）。在此基础上， 核心3的主要目标是优化和扩展BSL 3/ABSL 3资源 为学术界、工业界和联邦研究人员提供服务， 需求此外，核心3将提供研究服务，培训和专业知识，在更大的区域 生物防护实验室网络，同时还开发，验证和分发标准操作 进行BSL 3/ABSL 3研究的程序。为了实现这一目标，我们将优化Mtb的核心能力 研究，优化流感研究的核心能力，加强病媒-病原体相互作用方面的专门知识 研究，并通过更大的RBL网络伙伴关系发展核心竞争力。资助项目 由一组团队成员组成，他们具有开展涉及结核病、流感， 和蜱传疾病，包括：1）病原体的繁殖和分离; 2）使用几种实验 包括蜱攻击模型的动物模型系统; 3）医学对策筛选的应用; 4） 使用各种空气生物学方法; 5）评估相关的免疫生物标志物/结局 与感染因子;和6）整合病理学和基因组数据，包括空间 转录组学/蛋白质组学。总之，完成这些具体目标将加强总体的成果预算制 * 在强有力的公私伙伴关系/环境倡议响应所需的集体专门知识和能力方面进行协作， 并为更大的传染病研究社区提供资源工具箱。

PROJECT SUMMARY – CORE 3 The New England Regional Biosafety Laboratory (NERBL) was established in 2008 to: (1) ensure that research and tasks in response to pathogens of pandemic potential and other emerging infections (PPP/EI) are safely, efficiently, and rigorously accomplished; (2) serve as a regional resource for academic and industry partners engaged in activities that yield knowledge and medical countermeasures to PPP/EI; and (3) be ready to serve as directed by NIH/NIAID and their federal partners. A critical function of the NERBL includes the development and continuous improvement of specialized research functions unique to BSL3/ABSL3, particularly those focused on pathogens with human impact. At Tufts, existing expertise and knowledge bridges several areas of emphasis including bacteriology (tuberculosis [Mtb]), virology (influenza), and vector- pathogen interactions (eastern equine and Powassan encephalitis, tularemia). Building upon these fundamental capacities, the primary goal of Core 3 is to optimize and expand BSL3/ABSL3 resources and services for academic, industry and federal researchers to address both regional and national needs. Additionally, Core 3 will provide research services, training, and expertise across the larger Regional Biocontainment Laboratories network while also developing, validating and distributing standard operating procedures for conducting BSL3/ABSL3 research. To accomplish this, we will optimize core capacities for Mtb research, optimize core capacities for influenza research, enhance expertise in vector-pathogen interaction research, and grow core competencies through the larger RBL Network partnership. This work is supported by a set of team members with several key skills necessary for undertaking studies that involve Mtb, influenza, and tick-borne disease, including: 1) propagation and isolation of pathogens; 2) use of several experimental animal model system including tick challenge models; 3) application of medical countermeasure screening; 4) use of various aerobiology methodologies; 5) assessment of immunologic biomarkers/outcomes associated with infectious agents; and 6) integration of pathology and genomic data, including spatial transcriptomics/proteomics. Together, completion of these specific aims will strengthen the overall RBL collaborative with respect to collective expertise and capacities necessary for robust PPP/EI responsiveness, and provide a toolbox of resources for the larger infectious disease research community.