Gene expression profiling of leukemia cells traduced with mutated p16INK4A gene sensitive to temperature

利用温度敏感的p16 INK 4A诱导的白血病细胞系和Affytelm公司的12,000基因芯片，我们研究了不同温度下p16基因表达改变的基因表达谱。结果显示Rb 1、p107、p130、p300、DP-1、CDK 4、CDK 6、cyclin D1、cyclin E表达增加。CD 19、CD 83、T细胞白血病/淋巴瘤1A降低。感兴趣的是，多种蛋白体的表达通过上调或下调p16 RNA表达而改变。此外，我们研究了TSLP与或不与CD 40配体对白细胞的影响，发现吲哚胺2，3-双加氧酶（IDO）是一个重要因素。此外，研究了视黄酸对神经母细胞瘤细胞分化的影响。与白血病细胞一样，维甲酸影响N-myc、cyclin D34、Wnt 10 B以及RB、p107、p130、p300/CBP、E2 F-1、DP-1的表达。这些发现发表在Journal of Pediatric Hematology/Oncology 2003;25：715-720上。

Using temperature sensitive p16INK4A inducible leukemic cell lines and 12,000 gene chip by Affymetrix, we have investigated gene expression profiles altered by p16 gene expression under different temperature. As results, expressions of Rb1,p107,p130,p300,DP-1, CDK4,CDK6,cyclin D1,cyclin E were increased. In contrast, CD19,CD83,T cell leukemia/lymphoma 1A were decreased. Of interest, expressions of many kinds of proteosomes were altered by upregulation or by downregulation of p16 RNA expression. Moreover, we examined the effects of TSLP with or without CD40 ligand on leukocytes to find indoleamine 2,3-dioxygenase (IDO) as an important factor. Pediatric Research (in press).Moreover, effects of retinoic acid on differentiation of neuroblastoma cells were studied. Like leukemia cells, retinoic acid influenced the expressions of N-myc, cyclin D34,Wnt10B as well as RB, p107,p130,p300/CBP, E2F-1,DP-1. These findings have published in Journal of Pediatric Hematology/Oncology 2003;25:715-720.