Gene expression profiling of leukemia cells traduced with mutated p16INK4A gene sensitive to temperature

温度敏感突变 p16INK4A 基因转导的白血病细胞的基因表达谱

• 批准号: 15591137
• 负责人: URASHIMA Mitsuyoshi
• 金额: $ 2.37万
• 依托单位: Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591137/
• 关键词: oncosuppressor; leukemia; p16; proteosome; RNA; expression; TSLP; IDO; 神経芽細胞腫; DNAチップ; 細胞周期

Using temperature sensitive p16INK4A inducible leukemic cell lines and 12,000 gene chip by Affymetrix, we have investigated gene expression profiles altered by p16 gene expression under different temperature. As results, expressions of Rb1,p107,p130,p300,DP-1, CDK4,CDK6,cyclin D1,cyclin E were increased. In contrast, CD19,CD83,T cell leukemia/lymphoma 1A were decreased. Of interest, expressions of many kinds of proteosomes were altered by upregulation or by downregulation of p16 RNA expression. Moreover, we examined the effects of TSLP with or without CD40 ligand on leukocytes to find indoleamine 2,3-dioxygenase (IDO) as an important factor. Pediatric Research (in press).Moreover, effects of retinoic acid on differentiation of neuroblastoma cells were studied. Like leukemia cells, retinoic acid influenced the expressions of N-myc, cyclin D34,Wnt10B as well as RB, p107,p130,p300/CBP, E2F-1,DP-1. These findings have published in Journal of Pediatric Hematology/Oncology 2003;25:715-720.

我们利用温度敏感的p16INK4A可诱导白血病细胞系和Affymetrix公司的12000基因芯片，研究了不同温度下p16基因表达变化对基因表达谱的影响。结果显示，Rb1、p107、p130、p300、DP-1、CDK4、CDK6、细胞周期蛋白D1、细胞周期蛋白E的表达增加。而CD19、CD83、T细胞白血病/淋巴瘤1A则下降。有趣的是，通过上调或下调p16RNA的表达，许多蛋白酶体的表达都发生了变化。此外，我们检测了有或没有CD40配体的TSLP对白细胞的影响，发现吲哚胺2，3-双加氧酶(IDO)是一个重要的因素。此外，还研究了维甲酸对神经母细胞瘤细胞分化的影响。与白血病细胞一样，维甲酸影响N-myc、细胞周期蛋白D34、WNT10B以及Rb、p107、p130、p300/CBP、E2F-1、DP-1的表达。这些发现发表在《儿科血液学/肿瘤学杂志》2003；25：715-720上。

项目成果

Gene expression profiles of peripheral and cord blood mononuclear cells altered by thymic stromal lymphopoietin (TSLP)

胸腺基质淋巴细胞生成素 (TSLP) 改变外周血和脐带血单核细胞的基因表达谱

• 期刊: Pediatric Research (in press)
• 作者: Urashima M;et al.
• 通讯作者: et al.

Gene and Protein Expression Profiling During Differentiation of Neuroblastoma Cells Triggered by 13-cis Retinoic Acid

13-顺式视黄酸触发神经母细胞瘤细胞分化过程中的基因和蛋白质表达谱

• 发表时间: 2003
• 期刊: Journal of Pediatric Hematology/Oncology 25/9
• 作者: Yuza Y;Urashima M;et al.
• 通讯作者: et al.

Use of the curved linear-array echo endoscope to identify gastrorenal shunts in patients with gastric fundal varices

• DOI: 10.1055/s-2004-825658
• 发表时间: 2004-08-01
• 期刊: ENDOSCOPY
• 影响因子: 9.3
• 作者: Kakutani, H;Hino, S;Urashima, M
• 通讯作者: Urashima, M

CD147 and matrix metalloproteinase-2 protein expression as significant prognostic factors in esophageal squamous cell carcinoma

• DOI: 10.1002/cncr.20593
• 发表时间: 2004-11-01
• 期刊: CANCER
• 影响因子: 6.2
• 作者: Ishibashi, Y;Matsumoto, T;Urashima, M
• 通讯作者: Urashima, M

Infrared ray electronic endoscopy combined with indocyanine green injection for sentinel nodes of patients with gastric cancer.

红外线电子内镜联合吲哚菁绿注射液治疗胃癌患者前哨淋巴结。

• 发表时间: 2004
• 期刊: Br J Surg 91
• 作者: Nimura H;Urashima M;et al.
• 通讯作者: et al.