The study of the effect of preoperative heavy-ion irradiation on inhibition of local recurrence in patients with resectable pancreas cancer after curative resection.

我们测量了MIAPaCa（乳头状腺癌），Bx-Pc和Panc-1（浸润性导管癌）治疗的重离子治疗的存活分数。与X射线照射相比，重离子治疗的细胞杀伤效果不依赖于氧浓度。检测血管生成相关蛋白的表达。乳头状腺癌比浸润性导管癌血流丰富。Panc-1组VEGF-A和VEGFZR-2的表达高于MIAPaCa组。结论：宫颈切除术后VEGF-A和VEGFR-2的表达无明显变化。而低氧条件下VEGFR-3的表达增强，与淋巴结转移和局部复发有关。重离子治疗降低了VEGFR-3的表达。因此，重离子治疗可减少胰腺癌根治性切除术后的局部复发。我们用抗体芯片系统检测了736种蛋白质。重离子处理可增加细胞周期调控蛋白的表达。而缺氧条件下细胞周期调控蛋白的表达减少，损伤细胞的细胞周期延迟。在此期间，受损细胞能够修复DNA断裂。

We measured surviving fractions of MIAPaCa (papillary adenocarcinoma), Bx-Pc and Panc-1 (Invasive ductal carcinoma) treated by heavy-ion therapy. Cell killing effects of heavy ion therapy did not depend on the oxygen concentrations compared to that of X-ray irradiations. Then we measured the protein expressions related angiogenesis. Papillary adenocarcinoma has richer blood flow than invasive ducatal carcinoma. Expressions of VEGF-A and VEGFZR-2 at Panc-1 were higher that at MIAPaCa. Hypoxic concision did not change the expressions of VEGF-A and VEGFR-2. However hypoxic condition enhanced the expression of VEGFR-3 which was related to the lympho node metastasis and local recurrence. Heavy ion therapy reduced the expression of VEGFR-3. So heavy ion therapy reduced the local recurrence after curative resections in patients with pancreas cancer. We measured 736 proteins by Antibody Array System. Heavy ion therapy increased the expressions of cell cycle regulating proteins under oxic condition. However under hypoxic condition the expressions of cell cycle regulating proteins were decreased and cell cycle of damaged cell were delayed. During this delay damaged cell were able to repair DNA breaks.