The study of the effect of preoperative heavy-ion irradiation on inhibition of local recurrence in patients with resectable pancreas cancer after curative resection.

我们测量了经重离子治疗的 MIAPaCa（乳头状腺癌）、Bx-Pc 和 Panc-1（浸润性导管癌）的存活分数。与 X 射线照射相比，重离子治疗的细胞杀伤效果不依赖于氧气浓度。然后我们测量与血管生成相关的蛋白质表达。乳头状腺癌比浸润性导管癌具有更丰富的血流。 Panc-1 处 VEGF-A 和 VEGFZR-2 的表达高于 MIAPaCa 处。缺氧简洁并没有改变VEGF-A和VEGFR-2的表达。而缺氧条件下VEGFR-3的表达增强，与淋巴结转移和局部复发有关。重离子治疗降低了VEGFR-3的表达。因此，重离子治疗减少了胰腺癌患者根治性切除后的局部复发。我们通过抗体阵列系统测量了 736 种蛋白质。重离子治疗增加了有氧条件下细胞周期调节蛋白的表达。而在缺氧条件下，细胞周期调节蛋白的表达减少，受损细胞的细胞周期延迟。在此延迟期间，受损细胞能够修复 DNA 断裂。

We measured surviving fractions of MIAPaCa (papillary adenocarcinoma), Bx-Pc and Panc-1 (Invasive ductal carcinoma) treated by heavy-ion therapy. Cell killing effects of heavy ion therapy did not depend on the oxygen concentrations compared to that of X-ray irradiations. Then we measured the protein expressions related angiogenesis. Papillary adenocarcinoma has richer blood flow than invasive ducatal carcinoma. Expressions of VEGF-A and VEGFZR-2 at Panc-1 were higher that at MIAPaCa. Hypoxic concision did not change the expressions of VEGF-A and VEGFR-2. However hypoxic condition enhanced the expression of VEGFR-3 which was related to the lympho node metastasis and local recurrence. Heavy ion therapy reduced the expression of VEGFR-3. So heavy ion therapy reduced the local recurrence after curative resections in patients with pancreas cancer. We measured 736 proteins by Antibody Array System. Heavy ion therapy increased the expressions of cell cycle regulating proteins under oxic condition. However under hypoxic condition the expressions of cell cycle regulating proteins were decreased and cell cycle of damaged cell were delayed. During this delay damaged cell were able to repair DNA breaks.