Research on skeletal muscle reinforcement agent and the side effect

骨骼肌增强剂及其副作用的研究

• 批准号: 11680017
• 负责人: YAMADA Shigeru
• 金额: $ 2.3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680017/
• 关键词: Insulin; Muscle hypertrophy; muscle fiber type; Blood Component; Organ Weight; 筋線維組成; 臓器組織; 筋肥大; インスリン投与; ミオシン重鎖; 筋重量; ATPase染色; 筋線繊維型; 腱切除; マウス

Experiment I.The effect of drug administration on the skeletal muscle growth and compensatory muscle hypertrophy1. The body weight significantly decreased with the increase (especially, 8U/Kg administrated group 16.0 U/Kg administrated group) in the insulin dose.2. The blood glucose level of only 1.0U/kg insulin administrated group statistically significantly decreased compared it with Control group3. Soleus muscle weight of only 1.0U/kg administrated group was significantly higher than other group. Plantaris muscle weight dose not change significantly.4. The result of examining the effect of the compensatory load on the muscle weight soleus muscle weight increased 75%. The increment was statistically significantly. Though plantaris muscle weight increased average 30%, it was do not increase statistically. However, the soleus muscle weight of insulin administration group (2.0U/kg administrated group 16.0 U/kg administrated group) did not increase by the compensatory load. The increment … More of plantaris muscle weight in the 16U/kg insulin administrated group showed about 65% and the increment was statistically significant.Experiment II.The effect of insulin administration on muscle fiber composition in normal mice and muscle fiber composition of compensatory muscle hypertrophy.1. The percentage of fast muscle fiber of the soleus muscle increased with the increase in the insulin dose, but muscle fiber composition of plantaris muscle was not influenced. It was clarified that the effect of the insulin administration on the muscle fiber composition in slow and fast muscle was different.3. The percentage of slow muscle fiber of soleus muscle in the normal group increased by the compensatory load, but muscle fiber composition of plantaris muscle was not influenced by tenotomy. The result of examining the effect of insulin administration on the muscle fiber composition of compensatory muscle hypertrophy, the percentage of slow muscle fiber in coleus muscle increased. The percentage of slow muscle fiber of plantaris muscle increased in the 2.0 U/Kg insulin administrated group.Experiment III.The effect of drug administration on organ weight and bood component1. The weights of kidney, spleen, liver and heart changed statistically significant by insulin administration. However, the insulin administration did not influence the weight of lung and testis.2. The insulin administration did not affect each concentration of GOT, triacylglycerol, glucose in blood. Urea nitrogen concentration in blood statistically significantly decreased in only 4.0 U/kg administrated group compared it with the control group. Total cholesterol concentration in blood statistically significantly increased only the 8.0 U/kg group compared it with the control group. Creatinine concentration statistically significant increased in 2.0 U/kg insulin administrated group compared it with the control group. GPT concentration statistically significantly decreased in 1.0U/kg insulin administrated group compared it with the control group. Less

实验一：给药对骨骼肌生长和代偿性肌肥大的影响1。随着胰岛素剂量的增加（8 U/Kg给药组、16.0U/Kg给药组），体重显著下降.与对照组相比，仅给予1.0U/kg胰岛素组的血糖水平显著降低。1.0U/kg组比目鱼肌重显著高于其他组。跖肌重量变化不显著.对肌肉重量影响的研究结果表明，补偿负荷使比目鱼肌重量增加了75%。增量具有统计学显著性。跖肌重量平均增加30%，但无统计学意义。而胰岛素组（2.0U/kg给药组和16.0U/kg给药组）的比目鱼肌重量没有因代偿负荷而增加。增量 ...更多信息 16 U/kg胰岛素组足底肌重增加约65%，差异有统计学意义。实验二、胰岛素对正常小鼠肌纤维组成及代偿性肌肥大肌纤维组成的影响。比目鱼肌的快肌纤维百分比随胰岛素剂量的增加而增加，而跖肌的肌纤维组成不受影响。说明胰岛素对慢肌和快肌肌纤维组成的影响是不同的.正常组比目鱼肌慢肌纤维百分比随代偿负荷的增加而增加，而跖肌的肌纤维组成不受断腱的影响。研究胰岛素对补偿性肥大肌肉肌纤维组成的影响，结果表明：鞘蕊肌中慢肌纤维的百分比增加。2.0U/Kg胰岛素组大鼠跖肌慢肌纤维百分比增加。实验三、给药对脏器重量和血液成分的影响1.肾、脾、肝和心脏的重量在胰岛素给药后发生统计学显著变化。而胰岛素给药对肺和睾丸重量无影响.胰岛素给药不影响血中GOT、三酰甘油、葡萄糖的浓度。与对照组相比，仅4.0 U/kg剂量组的血液尿素氮浓度显著降低。与对照组相比，仅8.0 U/kg组血液中的总胆固醇浓度在统计学上显著增加。与对照组相比，2.0 U/kg胰岛素组肌酐浓度显著升高。与对照组相比，1.0U/kg胰岛素组的GPT浓度显著降低。少

项目成果

山田 茂: "運動と生体諸機能(森谷敏夫 編)"ナップ社. 254 (1999)

山田茂：“运动和各种生物功能（森谷俊夫编辑）”Knapp Publishing 254（1999）。

山田 茂: "インスリン投与が筋肥大及び筋腺維型に及ぼす影響"臨床スポーツ医学. (発売予定).

Shigeru Yamada：“胰岛素给药对肌肉肥大和肌纤维类型的影响”临床运动医学（待发布）。

山田 茂: "インスリン投与がヒト筋細胞の成長に及ぼす影響"臨床スポーツ医学. (発売予定).

Shigeru Yamada：“胰岛素给药对人体肌肉细胞生长的影响”临床运动医学（待发布）。