Clarification of the relationship between the cytokine gene polymorphism and the rejection/tolerance after liver transplantation

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BackgroundCytokine gene polymorphisms have been associated with cytokine production and transplant outcome in some studies. But only few studies have investigated the cytokine profile of patients weaning off immunosuppression. In this study we analyzed the association of gene polymorphisms in IL10,IL2,IL6,IFNγ and TNFα after weaning off immunosuppression in living donor liver transplant recipienta.Materials & methods:Liver transplant recipients who were successfully withdrawn from immunosuppression(n=19). A control group of liver transplant recipients who required maintenance of immunosuppression(n=30)Using the sequence specific primer PCR(PCR-SSP) method, the following cytokine gene polymorphisms were determined : IL10-592,IL10-819,IL10-1082,IL2-330,IL6-174,IFNγ+874 and TNFα-308.ResultOur study showed that Polymorphisms of low IFNγ+874 profile(G/A genotype) was associated with requiring prolonged immunsuppression.ConclusionPatients that require maintained immunosuppression are more likely to have a polymorphism of IFNγ(G/A genotype)This result is consistent with the proinflammatory properties of this cytokine, but previous reports showed no association with acute graft rejection in kidney and liver transplantation. So we need to analyse more sample and other cytokines.