Clarification of the relationship between the cytokine gene polymorphism and the rejection/tolerance after liver transplantation

阐明细胞因子基因多态性与肝移植术后排斥/耐受的关系

• 批准号: 15591350
• 负责人: ASONUMA Katsuhiro
• 金额: $ 2.11万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591350/
• 关键词: liver transplantation; tolerance; cytokine; SNP; 肝移植; 拒絶反応

BackgroundCytokine gene polymorphisms have been associated with cytokine production and transplant outcome in some studies. But only few studies have investigated the cytokine profile of patients weaning off immunosuppression. In this study we analyzed the association of gene polymorphisms in IL10,IL2,IL6,IFNγ and TNFα after weaning off immunosuppression in living donor liver transplant recipienta.Materials & methods:Liver transplant recipients who were successfully withdrawn from immunosuppression(n=19). A control group of liver transplant recipients who required maintenance of immunosuppression(n=30)Using the sequence specific primer PCR(PCR-SSP) method, the following cytokine gene polymorphisms were determined : IL10-592,IL10-819,IL10-1082,IL2-330,IL6-174,IFNγ+874 and TNFα-308.ResultOur study showed that Polymorphisms of low IFNγ+874 profile(G/A genotype) was associated with requiring prolonged immunsuppression.ConclusionPatients that require maintained immunosuppression are more likely to have a polymorphism of IFNγ(G/A genotype)This result is consistent with the proinflammatory properties of this cytokine, but previous reports showed no association with acute graft rejection in kidney and liver transplantation. So we need to analyse more sample and other cytokines.

背景 在一些研究中，细胞因子基因多态性与细胞因子的产生和移植结果相关。但只有很少的研究调查了停止免疫抑制的患者的细胞因子谱。本研究分析活体肝移植受者停止免疫抑制后IL10、IL2、IL6、IFNγ和TNFα基因多态性的相关性。材料与方法：成功退出免疫抑制的肝移植受者（n=19）。对照组需维持免疫抑制的肝移植受者(n=30)采用序列特异性引物PCR(PCR-SSP)法检测细胞因子基因多态性：IL10-592、IL10-819、IL10-1082、IL2-330、IL6-174、IFNγ+874、TNFα-308。 研究表明，低 IFNγ+874 谱（G/A 基因型）的多态性与需要长期免疫抑制相关。结论需要维持免疫抑制的患者更有可能具有 IFNγ（G/A 基因型）的多态性。该结果与该细胞因子的促炎特性一致，但之前的报道表明与肾脏急性移植排斥无关 和肝移植。所以我们需要分析更多的样本和其他细胞因子。

项目成果

Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation

• DOI: 10.1111/j.1600-6143.2004.00611.x
• 发表时间: 2004-12-01
• 期刊: AMERICAN JOURNAL OF TRANSPLANTATION
• 影响因子: 8.8
• 作者: Li, Y;Koshiba, T;Tanaka, K
• 通讯作者: Tanaka, K

Rescue for rare complications of the hepatic artery in living donor liver transplantation using grafts of autologous inferior mesenteric artery

• DOI: 10.1007/s00147-004-0763-y
• 发表时间: 2004-11-01
• 期刊: TRANSPLANT INTERNATIONAL
• 影响因子: 3.1
• 作者: Asonuma, K;Ohshiro, H;Inomata, Y
• 通讯作者: Inomata, Y

劇症肝不全の治療

暴发性肝衰竭的治疗

• 发表时间: 2003
• 期刊: 今日の移植 16(2)
• 作者: Okajima H;Pirenne J;吉元和彦;Okajima H;Pirenne J;Asonuma K;Lee KJ;Izaki T;Li Y;Kitade H;猪股裕紀洋;猪股裕紀洋;石河隆敏;Asonuma K;Ohashi M;Lee KJ;Izaki T;Li Y;Kitade H;Koshiba T;猪股裕紀洋
• 通讯作者: 猪股裕紀洋

Okajima H, Inomata Y, et al.: "Long-term effects of liver transplantation on bone mineral density in children with end-stage liver deisease : a 2-year prospective study"Liver Transplantation. 9.4. 360-364 (2003)

Okajima H、Inomata Y 等人：“肝移植对终末期肝病儿童骨矿物质密度的长期影响：一项为期 2 年的前瞻性研究”肝移植。

Regulatory cell-mediated tolerance does not protect against chronic rejection

• DOI: 10.1097/01.tp.0000080980.26287.11
• 发表时间: 2003-08-15
• 期刊: TRANSPLANTATION
• 影响因子: 6.2
• 作者: Koshiba, T;Kitade, H;Pirenne, J
• 通讯作者: Pirenne, J