The time of acquired tolerance formation in liver transplantation, and elucidation and clinical application of participating bioacctive substance

桥本是一名主要研究员，他于2004年4月与藤田健康大学医学院助理教授杉冈敦合作开始了这项研究。Sugioka助理教授从他之前的研究中澄清说，获得性耐受是在使用所有小鼠品系组装的实验性肝移植中衍生出来的。我们从2004年6月7日开始在藤田健康大学开展肝脏移植项目。目前，我们已经经历了9例活体供肝移植。虽然我们失去了一例23岁的女性血栓性血小板减少性紫癜，但剩下的8例是健康的，其中6例儿童有望获得耐受性。目前正在进行淋巴细胞交叉配型研究，并对血清标本进行定时保存。临床肝移植中一种免疫抑制相关体液因子的阐明我们最初计划使用蛋白质提示方法来阐明一种新的多肽。然而，很明显，SELDI…更多的蛋白质尖端需要花费大量的费用来检测一个样本。然后，我们没有获得关于这一过程的从头数据，除了直到现在的过去的值。为比较未使用免疫抑制剂的患者与门诊随访患者的免疫状态，我们选择并检测了免疫一致性细胞因子(IL-4、IL-8、IL-10、IL-12)，并对其进行评价。阻断FK506的获得性耐受为阐明小鼠肝移植后获得性耐受性的机制，阻断获得性耐受的获得有一定的参考价值。Sugioka教授已经证明，在小鼠肝移植中，在没有免疫抑制剂的情况下，在任何类型的组装中都可以得出获得性耐受。另一方面，我们知道获得性耐受是在肝移植后早期的严重排斥反应后发生的。因此，我们给予候选人FK506(他克莫司)，这是一种有效的免疫抑制剂，以阐明预防初始排斥反应的影响。这种治疗的结果是，我们能够防止排斥反应，但获得性耐受来源被阻止。较少

Hashimoto who was a main researcher started study in April 2004 in cooperation with assistant professor Atsushi Sugioka in Fujita Health University School of Medicine.Assistant professor Sugioka elucidates from his previous study that acquired tolerance is derivate in an experimental liver transplantation using all assembly of mouse strains.1. We started liver transplantation program in Fujita Health University from June 7 2004. Now, we have experienced 9 cases of living donor liver transplantation.Although we lost a case of 23 year old female from thrombotic thrombocytopenic purpura, but remaining 8 cases are healthy, and acquired tolerance derivation is expected in 6 of child cases. Now we are investigating the lymphocyte cross matching and storing the serum samples with time.2. Elucidation of an immunosuppression related humoral factors in a clinical liver transplantationWe initially planed to use a protein tip method to elucidate a new peptide. However, it becomes clear that SELDI … More protein tip needs a large amount of expense to examine one specimen. We then did not obtain a denovo data concerning this procedure except only past values until the present. For a purpose to compare immune status of cases without an immunosuppressive agent with other cases in follow up at the outpatient clinic, we choose and measure immunology coherence cytokines (IL-4, IL-8, IL-IL-10, IL-12) and are evaluating it.3. A block of derivation of acquired tolerance by FK506For an elucidation of a mechanism of acquired tolerance derivation easily after mouse liver transplantation, it will be useful to block the obtaining of tolerance. Professor Sugioka has proved that the acquired tolerance is concluded in any kind of assembly without administration of immunosuppressive agent on a liver transplantation of mouse. On the other hand, we know that acquired tolerance is delivered after sever rejection in early period of the post transplantation in this liver transplantation. Therefore, we gave FK506 (tacrolimus) which was effective immunosuppressive agent to clarify influence by preventing of initial rejection, to the candidate. Resulting of this treatment, we were able to prevent the rejection, but acquired tolerance derivation was blocked. Less