The time of acquired tolerance formation in liver transplantation, and elucidation and clinical application of participating bioacctive substance

肝移植获得性耐受形成时间及参与活性物质的阐明及临床应用

• 批准号: 15591890
• 负责人: HASHIMOTO Takashi
• 金额: $ 2.05万
• 依托单位: FUJITA HEALTH UNIVERSITY (2004)Nagoya City University (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591890/
• 关键词: acquired tolerance; liver transplantation; cytokine; immunosuprression; protein; FK506; 再灌流障害

Hashimoto who was a main researcher started study in April 2004 in cooperation with assistant professor Atsushi Sugioka in Fujita Health University School of Medicine.Assistant professor Sugioka elucidates from his previous study that acquired tolerance is derivate in an experimental liver transplantation using all assembly of mouse strains.1. We started liver transplantation program in Fujita Health University from June 7 2004. Now, we have experienced 9 cases of living donor liver transplantation.Although we lost a case of 23 year old female from thrombotic thrombocytopenic purpura, but remaining 8 cases are healthy, and acquired tolerance derivation is expected in 6 of child cases. Now we are investigating the lymphocyte cross matching and storing the serum samples with time.2. Elucidation of an immunosuppression related humoral factors in a clinical liver transplantationWe initially planed to use a protein tip method to elucidate a new peptide. However, it becomes clear that SELDI … More protein tip needs a large amount of expense to examine one specimen. We then did not obtain a denovo data concerning this procedure except only past values until the present. For a purpose to compare immune status of cases without an immunosuppressive agent with other cases in follow up at the outpatient clinic, we choose and measure immunology coherence cytokines (IL-4, IL-8, IL-IL-10, IL-12) and are evaluating it.3. A block of derivation of acquired tolerance by FK506For an elucidation of a mechanism of acquired tolerance derivation easily after mouse liver transplantation, it will be useful to block the obtaining of tolerance. Professor Sugioka has proved that the acquired tolerance is concluded in any kind of assembly without administration of immunosuppressive agent on a liver transplantation of mouse. On the other hand, we know that acquired tolerance is delivered after sever rejection in early period of the post transplantation in this liver transplantation. Therefore, we gave FK506 (tacrolimus) which was effective immunosuppressive agent to clarify influence by preventing of initial rejection, to the candidate. Resulting of this treatment, we were able to prevent the rejection, but acquired tolerance derivation was blocked. Less

主要研究人员Hashimoto于2004年4月与藤田保健大学医学院的Atsushi Sugioka助理教授合作开始研究。Sugioka助理教授从他之前的研究中阐明，获得性耐受是在使用所有小鼠品系的实验性肝移植中获得的。 1．我们从2004年6月7日开始在藤田保健大学开展肝移植项目。目前，我们已经经历了9例活体肝移植。虽然我们失去了1例因血栓性血小板减少性紫癜而失去的23岁女性，但剩下的8例都是健康的，并且预计6例儿童会获得获得性耐受。目前正在研究淋巴细胞交叉配型和血清样本随时间的保存。 2．临床肝移植​​中免疫抑制相关体液因子的阐明我们最初计划使用蛋白质尖端方法来阐明新的肽。然而，很明显，SELDI … More 蛋白质尖端需要大量费用来检查一个样本。然后，我们没有获得有关此过程的从头数据，除了迄今为止的过去值。为了比较未使用免疫抑制剂的病例与门诊随访的其他病例的免疫状态，我们选择并测量免疫学相干细胞因子（IL-4、IL-8、IL-IL-10、IL-12）并对其进行评估。 3． FK506阻断获得性耐受的产生对于阐明小鼠肝移植后容易获得的获得性耐受的产生机制，阻断耐受的获得将是有用的。 Sugioka教授通过小鼠肝移植证明，在不使用免疫抑制剂的情况下，任何类型的组装都会产生获得性耐受。另一方面，我们知道，在本次肝移植中，移植后早期发生严重排斥反应后会产生获得性耐受。因此，我们给予候选者FK506（他克莫司），它是一种有效的免疫抑制剂，以明确防止初始排斥反应的影响。通过这种治疗，我们能够防止排斥反应，但获得性耐受的产生被阻止。较少的

项目成果

Clinical diagnosis and treatment of the rejection on liver transplantation

肝移植排斥反应的临床诊治

• 发表时间: 2005
• 期刊: KAN・TAN・SUI (Japan) 50(1)
• 作者: Masahito Hibi;Takashi Hashimoto;Fujio Hara;Hirokazu Tomishige;Yoshihisa Nishida;Atsushi Sugioka;Takazumi Kato;Tetsushi Yoshikawa;Tatsuya Suzuki;Tanahashi Yoshinao;Tsukasa Nakamura
• 通讯作者: Tsukasa Nakamura

特集 生体肝移植とその短期および長期成績 I.ドナー 1.左肝切除

专题 活体肝移植及其近期和远期结果 一、供体 1. 左肝切除

• 发表时间: 2004
• 期刊: 外科 66(9)
• 作者: Masahito Hibi;Takashi Hashimoto;Fujio Hara;Hirokazu Tomishige;Yoshihisa Nishida;Atsushi Sugioka;Takazumi Kato;Tetsushi Yoshikawa;Tatsuya Suzuki;Tanahashi Yoshinao;Tsukasa Nakamura;Yuichi Shirasawa;棚橋 義直
• 通讯作者: 棚橋 義直

Postoperative corticosteroid therapy for bile drainage in biliary atresia - A nationwide survey

• DOI: 10.1016/j.jpedsurg.2004.08.019
• 发表时间: 2004-12-01
• 期刊: JOURNAL OF PEDIATRIC SURGERY
• 影响因子: 2.4
• 作者: Muraji, T;Nio, M;Ohi, R
• 通讯作者: Ohi, R

Long-term prognosis of left liver donor on the living donor liver transplantation

活体肝移植中左肝供者的远期预后

• 发表时间: 2004
• 期刊: Surgery 66(9)
• 作者: Tanahashi Yoshinao;Takashi Hashimoto;Tatsuya Suzuki;Tomoshi Kondo;Yoko Sato;Tsukasa Nakamura;Yoshinori Nakamura;Yota Yamagishi;Masahito Hibi;Takazumi Kato;Atsushi Sugioka
• 通讯作者: Atsushi Sugioka

特集 生体肝移植とその短期および長期成績I.ドナー 1.左肝切除

专题 活体肝移植及其近期和远期结果 一、供体 1. 左肝切除

• 发表时间: 2004
• 期刊: 外科 66(9)
• 作者: Toshihiro Muraji;Masaki Nio;Youkatsu Ohhama;Takashi Hashimoto;Tadashi Iwanaka;Hideo Takamatsu;Naomi Ohnuma;Tetsuo Kato;Ryoji Ohi;棚橋 義直
• 通讯作者: 棚橋 義直