Angiogenesis and Tumor Proliferation/Metastasis of Human Colorectal Cancer Cell Line SW620 Transfected with Endocrine Glands-Derived-Vascular Endothelial Growth Factor, As a New Angiogenic Factor

新型血管生成因子内分泌腺源性血管内皮生长因子转染人结直肠癌细胞株SW620的血管生成与肿瘤增殖/转移

• 批准号: 15591393
• 负责人: GOI Takanori
• 金额: $ 1.79万
• 依托单位: University of Fukui (2004)福井医科大学 (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591393/
• 关键词: Colorectal cancer; Angiogenesis; EG-VEGF; 肝転移; EG-VEGF遺伝子; 血管新生

Endocrine glands-derived-venous endothelial growth factor (EG-VEGF) was recently cloned as a new angiogenic factor that selectively acts on the endothelium of endocrine gland cells. The expression of EG-VEGF was comfirmed in all colorectal cancer cell lines. (On the other hand, the expression of EG-VEGF mRNA was not detected in colorectal normal mucosae.)Tumor proliferation(cecum, s.c.) was significantly higher in the EG-VEGF transfectants than in the control cells. Also, Liver metastatic ratio was higher in the EG-VEGF transfectants than in the control cells. In this study, EG-VEGF may lead to angiogenesis, promoting cell proliferation, and liver metastasis in colorectal cancers. And When the EG-VEGF gene-overexpressing colorectal cancer cell line had been treated with phosphorothioate antisense EG-VEGF oligonucleotides, angiogenesis and tumor growth were inhibited. Angiogenesis factor EG-VEGF indicates that it is a cancer-specific and possibly tissue-specific angiogenesis factor in the large intestine, and suggests that it can be targeted by a novel anti angiogenesis therapy.

内分泌腺源性静脉内皮生长因子（EG-VEGF）是最近克隆的一种新的血管生成因子，选择性作用于内分泌腺细胞的内皮。EG-VEGF在所有大肠癌细胞系中均有表达。(On而正常大肠粘膜组织中EG-VEGF mRNA无表达。肿瘤增殖（盲肠，s.c.）在EG-VEGF转染细胞中的表达显著高于对照细胞。同时，EG-VEGF转染细胞的肝转移率高于对照细胞。在这项研究中，EG-VEGF可能导致血管生成，促进细胞增殖，并在结直肠癌肝转移。硫代反义EG-VEGF寡核苷酸处理过表达EG-VEGF基因的大肠癌细胞株后，血管生成和肿瘤生长受到抑制。血管生成因子EG-VEGF表明它是大肠中的癌症特异性和可能的组织特异性血管生成因子，并且表明它可以被新的抗血管生成疗法靶向。