Sialyl Lewis X binding lectins on natural killer cells and their functions

自然杀伤细胞上唾液酸路易斯X结合凝集素及其功能

基本信息

批准号：
16590465
负责人：
MATSUMOTO Kojiro
金额：
$ 2.18万
依托单位：
Toho University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590465/
关键词：
natural killer cells sialyl Lewis X killer lectin-like receptor cell-mediated cytotoxicity tyrosine phosphorylation NKG2D CD94 NK細胞レクチン様レセプターシアリルルイスX 細胞障害活性

项目摘要

Natural killer (NK) cells mediate cytotoxicity through cell-surface receptors including lectin-like receptors. We have investigated whether sialyl Lewis X (sLeX) antigen, Neu5Acα2,3Galβ1,4 (Fucα1,3)GlcNAc-R, can bind to the lectin-like receptor on human NK-derived KHYG cells, using transferrin secreted by human hepatoma-derived HepG2 cells (Hep-TF), whose N-glycans are rich in α1,3-fucosylated bi-, tri-, and tetra-antennary complex types of N-glycans, and commercially available human transferrin (Nor-TF), which is comprised of bi-antennary N-glycans without α1,3-fucosylation.High sLeX-expressing erythroleukemia-derived K562 cells isolated from fucosyltransferase 3-transfected cells were 2.5 fold more susceptible than wild-type K562 cells to KHYG cells. Fluorescein isothiocyanate (FITC)-labeled Hep-TF bound 1.8 fold more strongly to KHYG cells than did FITC-labeled Nor-TF. The binding was suppressed by treatment with anti-NKG2D, anti-NKG2C, anti-CD94, and anti-CD 161 antibodies. FITC-labeled Hep-TF bound more strongly to human monocyte-derived U937 cells transfected with NKG2D and CD94 than to wild-type U937 cells. Moreover, tyrosine phosphorylation of a 17 kDa protein in the KHYG cells was enhanced by incubation on a Hep-TF coated plate and treatment with an anti-NKG2D antibody, but not by a Nor-TF coated plate and an anti-CD94 antibody.These results indicated that the interaction of sLeX antigen with the lectin-like receptors on NK cells induces cytotoxicity, which is mediated through a tyrosine-phosphorylated 17 kDa protein.

天然杀伤（NK）细胞通过包括教授样受体在内的细胞表面受体介导细胞毒性。我们已经研究了siAllyl Lewis X（Slex）抗原，Neu5ACα2,3GALβ1,4（FUCα1,3）GlcNAC-R是否可以使用人类NK衍生的Khhyg细胞上的讲座受体结合，使用人肝瘤的hepG2细胞（hepg2 fried an-fried Bi fribies）的hep-tfucs an-hep-fucs an-fried Bi fried Bi fried HepG2细胞（hep-fucs），其N-fcans and-f in-n-fcans in-n-fcans in-n-fcans。 n-糖果的三 - 和四静脉复杂类型，以及商业上可用的人类转铁蛋白（NOR-TF），由无α1,3-螺旋化的双期n-糖果组成。高表达嗜酸性的erythrololeukia drountythrolookiby farsifed trythroliqused K562细胞均来自富甲基蛋白酶基的iptersylys 3--富含质量均具有3.富含质量的细胞。2. K562细胞到khyg细胞。荧光素异硫氰酸荧光素（FITC）标记的HEP-TF结合1.8倍与khyg细胞的结合比FITC标记的NOR-TF更强烈。通过抗NKG2D，抗NKG2C，抗CD94和抗CD 161抗体的处理来抑制结合。与野生型U937细胞相比，FITC标记的HEP-TF更强烈地与用NKG2D和CD94翻译的人单核细胞衍生的U937细胞结合。此外，通过在HEP-TF涂层板上孵育并用抗NKG2D抗体进行处理，从而增强了Khyg细胞中17 kDa蛋白的酪氨酸磷酸化，从而增强了抗NKG2D抗体，而不是通过NOR-TF涂层的板和抗CD94抗体的抗体，这些结果表明，这些结果表明了与SLEX抗体相互作用的相互作用。通过酪氨酸磷酸化的17 kDa蛋白介导。