QRT-PCR ANALYSIS OF GENES INVOLVED IN SIALYL-LEWIS X SYNTHESIS

唾液酸-刘易斯 X 合成涉及的基因的 QRT-PCR 分析

• 批准号: 8363112
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363112
• 关键词: Biopsy; Epithelial Cells; Funding; Genes; Grant; Helicobacter pylori; Human; Infection; National Center for Research Resources; Patients; Principal Investigator; Production; RNA; Research; Research Infrastructure; Resources; Source; Stomach; Technology; Transcript; United States National Institutes of Health; cost; sialyl Lewis x

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Transcript levels for B3GlcNAc T5 and genes involved in the production of sialyl-Lewis x will be analyzed by qRT-PCR to investigate changes resulting from infection of gastric epithelial cells with two different strains of Helicobacter pylori. We will be isolating RNA from human gastric biopsies from infected and non-infected patients to determine transcript abundance of the selected gene set.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 将通过qRT-PCR分析B3 GlcNAc T5和参与唾液酸-Lewis x产生的基因的转录水平，以研究由两种不同幽门螺杆菌菌株感染胃上皮细胞引起的变化。 我们将从感染和非感染患者的胃活检组织中分离RNA，以确定所选基因集的转录丰度。