Development of recombinant vaccines against canine protozoan diseases by using canine herpes virus vector

利用犬疱疹病毒载体研制犬原虫疾病重组疫苗

• 批准号: 13556049
• 负责人: XUAN Xuenan
• 金额: $ 8.7万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13556049/
• 关键词: Babesia gibsoni; Neospora caninum; Canine herpesvirus; Vaccnia virus; Recombinant vaccine; Canine; Protozoan disease; Live vaccine; 大腸菌発現性; バキュロウイルス; SCIDマウス感染モデル; 大腸菌発現系; cDNAライブラリー

1.Development of canine herpesvirus vectorIn the present study, some virulent and non-essential genes, such as TK, gE and gI genes of canine herpesviruse were identified and cloned. Canine herpesviruse deleting these genes can grow in vitro, however their virulence reduced greatly in vivo. Therefore, these genes were proposed as targets for insertion of foreign immunogenic genes.2.Cloning of genes encoding immunodominant antigens of canine protozoan parasitesMany genes encoding immunodominat antigens of canine pathogenic protozoan parasites, Babesia gibsoni and Neospora caninum were identified, cloned and characterized. The main genes areas follows : B.gibosoni BgP29, BgP50, BgP76, BgTRAP, BgAMA-1. BgRAP-1, Bg12D3 ; N.caninum NcSAG1, NcSRS2, NcGRA7, NcPDI, NcRP1, NcHSP70.3.Construction of recombinant viruses expressing protozoan antigensRecombinant canine herpesvirus or vaccinia virus expressing BgP29, BgP50, BgP76, NcSAG1, NcSRS2 were constructed, and demonstrated that the recombinant antigens expressed by recombinant viruses were similar to their native antigens from parasites in antigenicity.4.Protective effects of recombinant vaccinesDogs immunized with above recombinant viruses produced partial protective immunity against B.gibsoni and N.caninum infections, respectively.

1.犬类疱疹病毒载体的开发是在本研究中，鉴定并克隆了犬疱疹的一些有毒和非必需的基因，例如犬疱疹的TK，GE和GI基因。这些基因删除这些基因的犬疱疹可以在体外生长，但是它们的毒力在体内大大降低。因此，提出了这些基因作为插入外源免疫原基因的靶标。2。编码犬类原生动物寄生虫的免疫抗原的基因，编码犬种病原体寄生虫的免疫神经抗原的犬类寄生虫基因，babesia gibsoni ggibsoni and neospora and neospora and and and an and and and and an and and and。主要基因领域如下：B.Gibosoni BGP29，BGP50，BGP76，BGTRAP，BGAMA-1。 Bgrap-1，BG12D3; N.CANINUM NCSAG1，NCSRS2，NCGRA7，NCPDI，NCRP1，NCRP1，NCHSP170.3.表达原生动物抗抗激素癌症的重组病毒的结构证明由重组病毒表达的重组抗原与抗原性中的寄生虫的天然抗原相似。4。重组疫苗染料对用上述重组病毒免疫的重组疫苗染料的保护作用，可产生对B.Gibsoni和N.Gibsoni和N. caninum carininum Inctection的部分保护性免疫。

项目成果

Fukumoto, S., et al.: "Identification and expression of a 50-kilodalton surface antigen of Babesia gibsoni and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay"J. Clin. Microbiol. 39・7. 2603-2609 (2001)

Fukumoto, S. 等人：“吉氏巴贝虫表面抗原的鉴定和表达及其在酶联免疫吸附测定中的诊断潜力”J. Clin 39·7。 (2001)

Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection.

• DOI: 10.1016/s0264-410x(00)00389-3
• 发表时间: 2001-12
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: Y. Nishikawa;N. Inoue;X. Xuan;H. Nagasawa;I. Igarashi;K. Fujisaki;H. Otsuka;T. Mikami

Nishikawa, Y., et al.: "Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation"Virus Res.. 87・1. 1-9 (2002)

Nishikawa, Y., et al.：“犬疱疹病毒 ORF2 是一种通过 N-连接糖基化修饰的膜蛋白”Virus Res.. 87・1 (2002)。

Fukumoto, S., et al.: "Development of a polymerase chain reaction method for diagnosing Babesia gibsoni infection in dogs"J. Vet. Med. Sci. 63・9. 977-981 (2001)

Fukumoto, S., et al.：“诊断犬吉布氏巴贝虫感染的聚合酶链反应方法的开发”J. Vet Med. 977-981 (2001)。

Fukumoto, S., et al.: "High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity"Clin.Diagn.Lab.Immunol.. 10・4. 596-601 (2003)

Fukumoto, S., et al.：“杆状病毒在昆虫细胞中高水平表达吉氏巴贝虫表面抗原 P50 及其免疫原性和抗原性的评估”Clin.Diagn.Lab.Immunol.. 10・4。 601（2003）