Isolation of cancer stem cells in bone and soft tissue tumors and novel molecular and cellular targeting therapy

骨和软组织肿瘤中癌症干细胞的分离以及新型分子和细胞靶向治疗

• 批准号: 20591765
• 负责人: HONOKI Kanya
• 金额: $ 2.75万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591765/
• 关键词: sarcoma; cancer stem cell; gene expression; drug resistance; osteosarcoma; mesenchymal stem cell; metastasis; stem cell niche; sarcosphere; DNA repair; INK4a

The current study indicates that rat and human sarcoma cells contain subpopulation of stem like cells with the formation of 'sarcoshpere'in anchorage-independent, serum starved culture condition, showing higher expression of stemness genes with the ability of self-renewal. Sarcosphere cells show the capacity of resistance to chemo-agents due to the up-regulation of DNA repair enzymes. These results suggest that the chemo-resistance in sarcomas might be caused in part by the presence of stem-like cell populations. These results suggest that targeting sarcoma stem cells could improve the prognosis of sarcoma patients.

目前的研究表明，大鼠和人肉瘤细胞在不依赖于锚定的血清饥饿培养条件下，含有可形成“sarcoshere”的干细胞亚群，具有较高的干性基因表达和自我更新能力。由于DNA修复酶的上调，肌球细胞显示出对化学药物的抵抗能力。这些结果表明，肉瘤中的化疗耐药性可能部分是由干细胞样细胞群的存在引起的。这些结果表明，靶向肉瘤干细胞可以改善肉瘤患者的预后。

项目成果

イホマイド脳症における危険因子の検討

碘霍胺脑病危险因素的检查

• 发表时间: 2009
• 作者: 藤井宏真;朴木寛弥;城戸顕;芳谷和洋;高倉義典
• 通讯作者: 高倉義典

Identification of sphere forming chemo-resistant stem-like cells in human Ewing sarcoma HTB166 cell line

人尤文肉瘤HTB166细胞系中球体形成化疗耐药干细胞的鉴定

• 发表时间: 2008
• 作者: Honoki;K;et al
• 通讯作者: et al

Human fibrosarcoma cell line HT1080lacking INK4a-ARF locus gene expression contains shere forming stem-like cells.

缺乏INK4a-ARF位点基因表达的人纤维肉瘤细胞系HT1080含有形成干细胞样细胞。

• 发表时间: 2008
• 作者: Honoki K;Fujii H;Tsujiuchi T;Kido A;Yoshitani K;Takakura Y
• 通讯作者: Takakura Y

Loss of lysophosphatidic acid receptor-3 enhances cell migration in rat lung tumor cells

• DOI: 10.1016/j.bbrc.2011.01.051
• 发表时间: 2011-02-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Hayashi, Mai;Okabe, Kyoko;Tsujiuchi, Toshifumi
• 通讯作者: Tsujiuchi, Toshifumi

低濃度ビスフォスフォネート製剤による造骨性肉腫細胞株の局所浸潤能の増強作用

低浓度双膦酸盐增强成骨肉瘤细胞系局部侵袭能力

• 发表时间: 2009
• 作者: 城戸顕;芳谷和洋;朴木寛弥;藤井宏真
• 通讯作者: 藤井宏真