Isolation of cancer stem cells in bone and soft tissue tumors and novel molecular and cellular targeting therapy

目前的研究表明，大鼠和人肉瘤细胞含有干细胞亚群，在非贴壁依赖性、血清饥饿培养条件下形成“肉瘤球”，表现出较高的干细胞基因表达，具有自我更新能力。由于DNA修复酶的上调，肌球细胞显示出对化学试剂的抵抗能力。这些结果表明，肉瘤中的化疗耐药性可能部分是由干细胞样细胞群的存在引起的。这些结果表明，靶向肉瘤干细胞可以改善肉瘤患者的预后。

The current study indicates that rat and human sarcoma cells contain subpopulation of stem like cells with the formation of 'sarcoshpere'in anchorage-independent, serum starved culture condition, showing higher expression of stemness genes with the ability of self-renewal. Sarcosphere cells show the capacity of resistance to chemo-agents due to the up-regulation of DNA repair enzymes. These results suggest that the chemo-resistance in sarcomas might be caused in part by the presence of stem-like cell populations. These results suggest that targeting sarcoma stem cells could improve the prognosis of sarcoma patients.