Effects of aging on the actions of general anesthetics in hippocampal slice

衰老对海马切片全身麻醉药作用的影响

• 批准号: 15591620
• 负责人: HIROTA Koki
• 金额: $ 2.3万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591620/
• 关键词: rat; hippocampus; aging; general anesthetics; excitatory synaptic transmission; inhibitory synaptic transmission

We compared age-related modifications of the effects of general anesthetics in area CA1 of hippocampal slices at 4 weeks (younger group) and 18 weeks (elder group) of age.Method. Transverse hippocampal slices (400 μm) were prepared from male Wistar rats. Two sets of bipolar electrodes were placed on Schaffer collateral fibers to elicit population spikes (PS) in area CA1 [Sch], and on alveus hippocampi to activate the inhibitory interneurons for GABAergic modulation [Alv].Results. Volatile anesthetics (isoflurane, sevoflurane) decreased PS[Sch], while intravenous anesthetics (thiopental, propofol) had a minimal effects on PS[Sch]. The extents of inhibitory effects by volatile anesthetics were similar between younger and elder groups. On the other hand, both volatile and intravenous anesthetics reduced PS[Sch+Alv]. The actions were grater on younger group than on elder group.Conclusion. We demonstrated the age-dependent modification of general anesthetic. actions on the inhibitory synaptic transmission, but not on the excitatory synaptic transmission, in rat CNS preparations in vitro.

我们比较了4周龄（年轻组）和18周龄（老年组）时全身麻醉药对海马脑片CA1区作用的年龄相关性改变。从雄性Wistar大鼠制备横向海马切片（400 μm）。将两组双极电极分别置于Schaffer侧支纤维上，在CA1区[Sch]诱发群体锋电位（PS），并在肺泡上诱发抑制性中间神经元[Alv]诱发GABA能调制。挥发性麻醉剂（异氟烷、七氟烷）降低PS[Sch]，而静脉麻醉剂（硫喷妥钠、丙泊酚）对PS[Sch]的影响极小。挥发性麻醉药的抑制作用程度在年轻组和老年组之间相似。另一方面，挥发性和静脉麻醉剂均降低PS[Sch+Alv]。青年组较老年组行为更明显。我们证明了全身麻醉药的年龄依赖性修改。在离体大鼠中枢神经系统中，对抑制性突触传递有作用，但对兴奋性突触传递无作用。

项目成果

Anoxic depolarization of rat hippocampal slices is prevented by thiopental but not by propofol or isoflurane.

硫喷妥钠可以防止大鼠海马切片的缺氧去极化，但丙泊酚或异氟烷则不能。

• 发表时间: 2005
• 期刊: British Journal of Anaesthesia 94
• 作者: R.Sasaki;K.Hirota;et al.
• 通讯作者: et al.

麻酔科スタンダード(小川節郎, 新宮 興, 武田純三, 西野 卓 編)

麻醉学标准（小川节郎、新宫彰、武田顺三、西野隆主编）

• 发表时间: 2004
• 作者: R.Sasaki;K.Hirota;et al.;広田弘毅
• 通讯作者: 広田弘毅

マグネシウムの基礎と臨床(外 須美夫 編集)

镁的基础知识和临床实践（Sumio Soto 编辑）

• 发表时间: 2005
• 作者: 廣田弘毅;佐々木利佳
• 通讯作者: 佐々木利佳

Thiopentalの反回性抑制効果に及ぼす加齢の影響

衰老对硫喷妥钠复发抑制作用的影响

• 发表时间: 2005
• 期刊: 麻酔と蘇生 41
• 作者: 佐々木利佳;広田弘毅;他
• 通讯作者: 他

The effects of mannitol and hypothermia on spontaneous spreading depression induced by anoxia in the rat hippocampal CA1.

甘露醇和低温对缺氧引起的大鼠海马CA1自发性抑郁症的影响。

• 发表时间: 2003
• 期刊: Anesthesia and Resuscitation 39
• 作者: R.Sasaki;K.Hirota;et al.
• 通讯作者: et al.