Interaction of sarcolemmal and mitochondrial ATP-sensitive K channel on cardioprotection
肌膜和线粒体 ATP 敏感 K 通道的相互作用对心脏保护作用
基本信息
- 批准号:15591636
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Ischemic Preconditioning is a phenomenon in which brief intermittent periods of ischemia are paradoxically protective against subsequent ischemic injury. To characterize the interaction sarcolemmal and mitochondrial ATP-sensitive K channel (KATP) on cardioprotection, we studied pretreatment effects of 2,4-dinitrophenol, a protonophore that uncouples mitochondrial respiration from ATP synthesis, on both flavoprotein oxidation, an index of mitochondrial uncoupling and sarcolemmal KATP activation in isolated rat ventricular myocytes. Effects of 5-hydroxydecanoic acid, a mitochondrial KATP inhibitor, on the pretreatment effects were also studied. Major findings of this study were that pretreatment of mitochondrial uncoupler sensitizes flavoprotein oxidation and sarcolemmal KATP activation. In the presence of 5-hydroxydecanoic acid the sensitizing effects were completely abolished. Without metabolic inhibition, sensitized myocardium represents almost normal flavoprotein oxidation and sarcol … More emmal KATP activity. These results suggest that, if we assume a memory molecule, direct effects of the memory molecule potentiates mitochondrial uncoupling and sarcolemmal KATP are negligible, but the memory molecule potentates mitochondrial uncoupling and sarcolemmal KATP in the face of metabolic impairment. In addition, the production of the memory molecule is 5-hydroxydecanoic acid-sensitive.Next, we evaluated the effects of isoflurane on the K_<ATP> channel activities in cultured rat smooth muscle cells. Openings of K_<ATP> channels were identified on the basis of the characteristic single-channel conductance (28±4pS), activation by 100 μM pinacidil, and block by 3 μM glibenclamide in cell-attached configuration. Application of isoflurane to bath solution during cell-attached recording significantly activated K_<ATP> channels. In contrast to cell-attached patches, isoflurane did not induced activation on K_<ATP> channel currents in outside-out patchclamp configuration. Isoflurane induced activation of K_<ATP> channels was abolished by a combination of PKA inhibitor peptide. Our results indicated that isoflurane activated K_<ATP> channels in rat smooth muscle cells via PKA activation K_<ATP> channel currents Less
缺血预适应是一种现象,在这种现象中,短暂的间歇性缺血对随后的缺血损伤具有矛盾的保护作用。为了研究肌膜和线粒体ATP敏感性钾通道(KATP)相互作用对心肌的保护作用,我们研究了2,4-二硝基酚对大鼠心肌细胞黄素蛋白氧化和肌膜KATP激活的影响。此外,还研究了线粒体KATP抑制剂5-羟基癸酸对预处理效果的影响。本研究的主要发现是线粒体解偶联蛋白的预处理使黄素蛋白的氧化和肌膜KATP的激活变得敏感。在5-羟基癸酸存在下,增敏作用完全消除。在没有代谢抑制的情况下,致敏心肌代表几乎正常的黄素蛋白氧化和肌醇…更多的KATP活性。这些结果表明,如果我们假设一个记忆分子,记忆分子的直接作用是加强线粒体解偶联,而肌膜KATP可以忽略不计,但在代谢受损的情况下,记忆分子增强了线粒体解偶联和肌膜KATP。此外,记忆分子的产生是对5-羟基癸酸敏感的。接下来,我们观察了异氟烷对培养的大鼠血管平滑肌细胞K_<;ATP>;通道活性的影响。根据单通道电导(28±4ps)、100μM吡那地尔激活和3μM格列本脲阻断细胞贴壁构型,确定K_1通道的开放。在细胞贴壁记录过程中,在浴液中加入异氟醚可显著激活钾通道。与细胞贴附的膜片不同,异氟醚在外向式膜片钳结构中不能引起钾通道电流的激活。异氟醚对K<;ATP和Gt;通道的激活作用可被PKA抑制肽联合阻断。我们的结果表明,异氟醚通过激活PKA激活大鼠血管平滑肌细胞上的钾通道,通道电流较少。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Molecular mechanisms of the inhibitory effects of propofol and thiamylal on sarcolemmal adenosine triphosphate-sensitive potassium channels
- DOI:10.1097/00000542-200402000-00024
- 发表时间:2004-02-01
- 期刊:
- 影响因子:8.8
- 作者:Kawano, T;Oshita, S;Nakaya, Y
- 通讯作者:Nakaya, Y
Molecular mechanisms of the inhibitory effects of bupivacaine, levobupivacaine, and ropivacaine on sarcolemmal adenosine triphosphate-sensitive potassium channels in the cardiovascular system
- DOI:10.1097/00000542-200408000-00020
- 发表时间:2004-08-01
- 期刊:
- 影响因子:8.8
- 作者:Kawano, T;Oshita, S;Nakaya, Y
- 通讯作者:Nakaya, Y
Kawano T, et al.: "Molecular mechanisms of inhibitory effects of propofol and thiamylal on sarcolemmal adenosine triphosphate-sensitive potassium channels"Anesthesiology. 100. 338-346 (2004)
Kawano T 等人:“丙泊酚和硫淀粉醛对肌膜三磷酸腺苷敏感钾通道抑制作用的分子机制”麻醉学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
OSHITA Shuzo其他文献
OSHITA Shuzo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('OSHITA Shuzo', 18)}}的其他基金
Isoflurane-induced postconditioning is mediated by activation of mitochondrial calcium-activated potassium channels
异氟烷诱导的后处理是通过线粒体钙激活钾通道的激活介导的
- 批准号:
21591975 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Does extraoellular potassium ion, which accumulates during myocardial is Ghemia, suppress the inorease of intraGelluIar calciumion?
心肌缺血时积累的细胞外钾离子是否抑制细胞内钙离子的增加?
- 批准号:
19591801 - 财政年份:2007
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The role of mitochondrial ATP sensitive potassium channel on cardioprotection of ischemic preconditioning and anesthetics
线粒体ATP敏感钾通道对缺血预适应和麻醉药心脏保护的作用
- 批准号:
13671586 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The role of K_<ATP> channel activities on the ischemic preconditioning and the influence of anesthetics on K_<ATP> channel activities.
K_<ATP>通道活性对缺血预处理的作用及麻醉药对K_<ATP>通道活性的影响。
- 批准号:
11671501 - 财政年份:1999
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Combined Effects of Acidosis, Hypoxia, and Anesthetics on the K_<ATP> Channels in Isolated Rat Myocytes.
酸中毒、缺氧和麻醉对离体大鼠肌细胞 K_<ATP> 通道的综合影响。
- 批准号:
09671568 - 财政年份:1997
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Determinants of cardioprotection by circulating prohibitin-1 during sepsis
败血症期间循环抑制素 1 的心脏保护作用的决定因素
- 批准号:
10577340 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Cardioprotection against Adverse Side-Effects of Ibrutinib in Cancer Chemotherapy
癌症化疗中依鲁替尼不良副作用的心脏保护作用
- 批准号:
487140 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Operating Grants
Targeting Immune-Responsive Gene 1 (Irg1) and Itaconate for Cardioprotection of the Donor Heart for Transplantation
靶向免疫反应基因 1 (Irg1) 和衣康酸对移植供体心脏进行心脏保护
- 批准号:
10895712 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Circadian Rhythm as a Therapeutic Target for Perioperative Cardioprotection
昼夜节律作为围手术期心脏保护的治疗目标
- 批准号:
10659089 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Improvement of the intraoperative cardioprotection by SGLT2 inhibitor in the era of increasing diabetic patients
糖尿病患者增多时代SGLT2抑制剂改善术中心脏保护作用
- 批准号:
23K08281 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Sex Differences in Endogenous Formaldehyde Production and One-Carbon Metabolism: Potential Links to Cardioprotection
内源性甲醛产生和一碳代谢的性别差异:与心脏保护的潜在联系
- 批准号:
10894590 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
Application of aldosterone antagonist to intraoperative cardioprotection
醛固酮拮抗剂在术中心脏保护中的应用
- 批准号:
22K08965 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cardioprotection during hypertensive heart disease is mediated through anti-viral cytokine pathways
高血压心脏病期间的心脏保护是通过抗病毒细胞因子途径介导的
- 批准号:
461883 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
Operating Grants
Sex Differences in Endogenous Formaldehyde Production and One-Carbon Metabolism: Potential Links to Cardioprotection
内源性甲醛产生和一碳代谢的性别差异:与心脏保护的潜在联系
- 批准号:
10536259 - 财政年份:2022
- 资助金额:
$ 2.24万 - 项目类别:
Therapeutic strategy for heart failure with preserved ejection fraction: Analysis of the signaling pathway involved in the cardioprotection through inter-organ interactions
射血分数保留性心力衰竭的治疗策略:通过器官间相互作用参与心脏保护的信号通路分析
- 批准号:
21K06810 - 财政年份:2021
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)