Analysis of distribution of dihydrotestosterone concentration in prostate cancer tissue and differences of dihydrotestosterone and androgen induced gene expression after androgen deprivation

目的：分子研究表明，种族影响前列腺肿瘤生物学。我们评估了多个已知的预后标志物在本地化的前列腺癌组织微阵列（TMA）在日本patients.Methods：标本来自52例患者接受根治性手术在我们的机构在1997年和2001年之间没有新辅助激素治疗，完整的3个或更多可用的癌症斑点。检测Ki67、p53和雄激素受体（AR）抗原表达。结果：病理肿瘤（T）分类（p=0.047），世界卫生组织（WHO）评分（p= 0.026），WHO组织学分级（p = 0.026），手术切缘状态（p =0.018）是预测生化失败的重要传统临床病理变量。TMA Gleason总和（p=0.038）、TMA原发Gleason分级（p=0.013）、Ki67标记指数（LI）（p<0.0001）、p53（p=0.0097）和AR（p=0.0113）抗原表达也是显著的。此外，手术切缘状态和Ki67 LI独立相关治疗failure.Conclusions：特别是在一起，Ki67 LI和p53和AR表达在一个本地化的前列腺癌往往预测术后进展的日本患者。

Objectives : Molecular studies suggest that ethnicity influences prostate tumor biology. We evaluated multiple known prognostic markers in localized prostate cancers using tissue microarrays (TMA) in Japanese patients.Methods : Specimens were from 52 patients undergoing radical surgery at our institution between 1997 and 2001 without neoadjuvant hormonal therapy with complete 3 or more available cancer spots. Ki67, p53, and androgen receptor(AR) antigen expression were examined. Immunohistochemical scores were compared with outcomes of chemical relapse as monitored using prostate-specific antigen(PSA).Results : Pathologic tumor (T) classification (p=0.047), World Health Organization(WHO) score (p=0.026), WHO histologic grade (p=0.026), and surgical margin status (p=0.018) were significant conventional clinicopathologic variables for predicting biochemical failure. TMA Gleason sum (p=0.038), TMA primary Gleason grade (p=0.013), Ki67 labeling index (LI) (p<0.0001), p53 (p=0.0097), and AR (p=0.0113) antigen expression also were significant. Moreover, surgical margin status and Ki67 LI were independently associated with treatment failure.Conclusions : Especially together, Ki67 LI and p53 and AR expression in a localized prostate cancer often predicted postoperative progression in Japanese patients.