Monitoring of CD15s Ag in Clinical Renal Tx and xenogereic model.

临床肾 Tx 和异种模型中 CD15s Ag 的监测。

• 批准号: 15591717
• 负责人: ISHIDA Hideki
• 金额: $ 1.54万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591717/
• 关键词: CD15s antigen; blood type incompatible; Renal Transplantation; Xonotransplantation; immuno suppressive regimen; CD15S抗原; 血液型不適合腎移植; 免疫抑制剤の調節

Renal transplantation across the blood barrier is a unique model for investigating the humoral response to different carbohydrate antigens. However, in such a renal transplantation, the characteristics of B cells as well as of the antibodies produced by B cells are less well defined.In the present study we investigated B cell subsets, i.e., the CD5(+) B-1 and CD5(-) B-2 subsets, by flow cytometric analysis, and their subclasses of antibody, by ELISA, in patients who had undergone renal transplantation across the blood barrier. The subjects consisted of five recipients with good function (group 1) and five recipients with graft loss (group 2) accompanied by antibody-titer elevation after ABO-incompatible renal transplantation.The B-cell population analysis revealed that CD5(+) B-1 cells temporarily increased in all patients in both groups soon after transplantation, and that CD5(-) B-2 cells significantly increased one month after transplantation only in group 2. The antibody subclasses analysis showed mild elevation of IgG2 and IgM in group 1 as opposed to remarked elevation of IgG2,IgM and IgG1 in group 2.

跨血屏障肾移植是研究不同碳水化合物抗原的体液反应的独特模型。然而，在这样的肾移植中，B细胞的特性以及B细胞产生的抗体都不太明确。在本研究中，我们通过流式细胞术分析研究了B细胞亚群，即CD5(+) B-1和CD5(-) B-2亚群，并通过ELISA检测了它们的抗体亚类，这些患者接受了跨血屏障肾移植。研究对象包括5例功能良好的受体（1组）和5例abo血型不相容肾移植术后伴有抗体滴度升高的移植物丧失受体（2组）。b细胞群分析显示，两组患者的CD5(+) B-1细胞在移植后不久短暂增加，而CD5(-) B-2细胞仅在移植后1个月显著增加。抗体亚类分析显示，IgG2和IgM在1组中轻度升高，而IgG2、IgM和IgG1在2组中明显升高。

项目成果

Therapeutic apheresis for ABO incompatible renal transplantat

ABO血型不合肾移植的治疗性单采术

• 发表时间: 2003
• 期刊: Therapeutic Apheresis 7(6)
• 作者: Hideki ISHIDA;et al.;石田英樹
• 通讯作者: 石田英樹

Hepatitis C virus dealeses in patients with maintainance hemofiltration therapy

丙型肝炎病毒在维持性血液滤过治疗患者中传播

• 发表时间: 2004
• 期刊: Artificial Organs 28
• 作者: Hideki ISHIDA;et al.
• 通讯作者: et al.

石田英樹 ら: "日本の腎移植の現状と問題点"慢性腎不全(新しい診断と治療のABC) 最新医学社. 初. 184-190 (2003)

Hideki Ishida 等：“日本肾移植的现状和问题”慢性肾衰竭（新诊断和治疗的 ABC） Shinsei Igakusha 第一。

Organ donation problems in Japan

日本的器官捐献问题

• 发表时间: 2004
• 期刊: Saudi Kidney Dis Transplant 15
• 作者: Hideki ISHIDA;et al.;石田英樹;石田英樹;石田英樹
• 通讯作者: 石田英樹

Efficiency of IgM anti-blood type Ab by ELISA

通过 ELISA 检测 IgM 抗血型抗体的效率

• 发表时间: 2004
• 期刊: Transplant Proceeding 36
• 作者: Hideki ISHIDA;et al.;石田英樹
• 通讯作者: 石田英樹