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Analysis of chemokine-chemokine receptor system in human female reproductive tissue and its function

人类女性生殖组织趋化因子-趋化因子受体系统及其功能分析

基本信息

批准号：
15591746
负责人：
SHIMOYA Koichiro
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Fractalkine is a CX3C chemokine that has chemoattractant activity for T cells, monocytes and natural killer cells. Western blot analysis revealed that fractalkine protein was detected in the all parts of the Fallopian tube. Immunohistochemical analysis revealed positive staining of the epithelial cells in the oviduct. RT-PCR demonstrated that CX3CR1 transcripts were expressed in the Fallopian tube. RT-PCR also revealed that CX3CR1 positive cells were present in the tube. CX3CR1-positive cells were present in the stroma of the Fallopian tube. RT-PCR demonstrated that CX3CR1 transcripts were expressed in the ejaculated sperm. Immunohistochemical analysis demonstrated positive staining of the tail of the spermatozoa. These results suggest that fractalkine in the Fallopian tube contributes to the immunodefence mechanism during fertilization and to the sperm motion in the oviduct. Fractalkine protein was detected in the ascites. Positive staining was confirmed in peritoneal surface cells and perivascular cells of the peritoneum. CX_3CR1 positive cells were present in the cells in the peritoneal fluid.We examined the association between fractalkine in the peritoneal fluid and endometriosis. The fractalkine concentrations in the peritoneal fluid with endometriosis were lower than those without endometriosis. There was no significant difference between serum fractalkine levels with and without endometriosis. These results suggested that decreased levels of fractalkine found in the peritoneal fluid of patients with endometriosis may contribute to the pathogenesis of endometriosis.We also examined the expression of fractalkine in the endometrium of the uterus and the uterine cervix. Western blot analysis revealed that fractalkine were present in both tissues. RT-PCR also demonstrtated that fractalkine transcripts were present in these organs. These results suggested that fractalkine might play an important role to protect against invasive pathogens in the uterine cavity.

Fractalkine是一种CX 3C趋化因子，对T细胞、单核细胞和自然杀伤细胞具有趋化活性。Western blot分析显示，Fractalkine蛋白在输卵管的各个部位都有表达。免疫组化显示输卵管上皮细胞呈阳性染色。RT-PCR结果显示，CX 3CR 1基因在输卵管中有表达。RT-PCR也显示CX 3CR 1阳性细胞存在于管中。CX 3CR 1阳性细胞存在于输卵管间质中。RT-PCR结果显示CX 3CR 1基因在精子中有表达。免疫组化显示精子尾部呈阳性染色。这些结果表明，输卵管中的fractalkine有助于受精过程中的免疫防御机制和精子在输卵管中的运动。腹水中检测到Fractalkine蛋白。阳性染色在腹膜表面细胞和腹膜血管周围细胞中证实。腹腔液中CX_3CR1阳性细胞的表达与子宫内膜异位症的发生有关。子宫内膜异位症患者腹腔液中Fractalkine浓度低于非子宫内膜异位症患者。子宫内膜异位症与非子宫内膜异位症患者血清Fractalkine水平无显著差异。这些结果表明，子宫内膜异位症患者腹腔液中Fractalkine水平的降低可能有助于子宫内膜异位症的发病机制。我们还研究了子宫内膜和子宫颈中Fractalkine的表达。Western blot分析显示，fractalkine存在于两种组织中。RT-PCR也证实了Fractalkine转录本在这些器官中的存在。提示Fractalkine可能在保护子宫免受入侵性病原体的侵袭中发挥重要作用。