Analysis of function of protease inhibitor in the human reproduction area and its clinical application

蛋白酶抑制剂在人类生殖领域的作用分析及临床应用

• 批准号: 13671713
• 负责人: SHIMOYA Koichiro
• 金额: $ 2.24万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671713/
• 关键词: protease inhibitor; Fallopian tube; SLPI; acrosome reaction; fraclalkine; SLPI(secretory leukocyte elastase inhibitor)

In the present study we examined the function of fractalkine in the reproductive area. Western blot analysis revealed the presence of fractalkine protein in the seminal plasma and the association between the fractalkine concentration of the seminal plasma and sperm mortality. We demonstrated the existence of fractalkine in the Fallopian tubes the localization of fractalkine in the epithelial cells of the Fallopian tubes. RT-PCR revealed the expression of fractalkine mRNA in the Fallopian tubes. We demonstrated the presence of CX3CR1 (fractalkine receptor) and the localization of CX3CR1 in the tails of the sperm. These results suggest that fractalkine in the Fallopian tube may have an important role in the sperm motion.

在本研究中，我们研究了fractalkine在生殖领域的功能。蛋白质印迹分析揭示了精浆中fractalkine蛋白的存在以及精浆中fractalkine浓度与精子死亡率之间的关联。我们证明了Fractalkine在输卵管中的存在，Fractalkine在输卵管上皮细胞中的定位。RT-PCR显示Fractalkine mRNA在输卵管中有表达。我们证明了CX 3CR 1（fractalkine受体）的存在和CX 3CR 1在精子尾部的定位。提示输卵管内Fractalkine可能在精子运动中起重要作用。

项目成果

Shimoya, K., et al.: "Diagnosis of ureterocele with transvaginal sonography."Gynecol Obstet Invest. 54. 58-60 (2002)

Shimoya, K. 等人：“经阴道超声诊断输尿管囊肿。”Gynecol Obstet Invest。

Shimoya, K., et al.: "Cervical fetal fibronectin(fFN)as a predictor of abnormal early pregnancy-Is that affected by blood contamination?"J Reprod Med. (in press).

Shimoya, K. 等人：“宫颈胎儿纤连蛋白 (fFN) 作为异常早期妊娠的预测因子 - 是否受到血液污染的影响？”J Reprod Med。

Shimoya, K., et al.: "Fractalkine(FRK) levels in amniotic fluid and its production during pregnancy"Mol Hum Reprod. 9(2). 97-101 (2003)

Shimoya, K. 等人：“羊水中的分形蛋白 (FRK) 水平及其在怀孕期间的产生”Mol Hum Reprod。

Ota, Y., Shimoya, K., et al.: "The expression of secretory leukocyte protease inhibitor(SLPI) in the fallopian tube: SLPI protects the acrosome reaction of sperm from inhibitory effects of elastase"Hum Reprod. 17(10). 2517-2522 (2002)

Ota, Y.、Shimoya, K. 等人：“分泌性白细胞蛋白酶抑制剂 (SLPI) 在输卵管中的表达：SLPI 保护精子的顶体反应免受弹性蛋白酶的抑制作用”Hum Reprod。

Shimoya, K., et al.: "Cervical fluid oncofetal firbronectin as a predictor of early etopic pregnancy Is it affected by blood contamination?"J Reprod Med. 47(8). 640-644 (2002)

Shimoya, K. 等人：“宫颈液癌胎儿纤连蛋白作为早期异位妊娠的预测因子，它是否受到血液污染的影响？”J Reprod Med。