Analysis of function of protease inhibitor in the human reproduction area and its clinical application

在本研究中，我们研究了Fractalkine在生殖区的功能。Western印迹分析显示，精浆中存在Fractalkine蛋白，精浆中Fractalkine浓度与精子死亡率之间存在关联。我们证实了Fractalkine在输卵管中的存在，以及Fractalkine在输卵管上皮细胞中的定位。RT-PCR检测到Fractalkine基因在输卵管中的表达。我们证明了CX3CR1(Fractalkine Receptor)的存在以及CX3CR1在精子尾部的定位。这些结果表明，输卵管中的Fractalkine可能在精子运动中起着重要作用。

In the present study we examined the function of fractalkine in the reproductive area. Western blot analysis revealed the presence of fractalkine protein in the seminal plasma and the association between the fractalkine concentration of the seminal plasma and sperm mortality. We demonstrated the existence of fractalkine in the Fallopian tubes the localization of fractalkine in the epithelial cells of the Fallopian tubes. RT-PCR revealed the expression of fractalkine mRNA in the Fallopian tubes. We demonstrated the presence of CX3CR1 (fractalkine receptor) and the localization of CX3CR1 in the tails of the sperm. These results suggest that fractalkine in the Fallopian tube may have an important role in the sperm motion.