Estimation of cancer susceptibility and risk of head and neck carcinoma by gene polymorphism and expression.

在代谢致癌物、修复DNA损伤和诱导细胞凋亡的能力上的遗传差异可能是导致总体人群癌症风险差异的原因之一。DNA修复基因的能力可能在烟草相关癌症的发生中起重要作用。我们推测XPD/ERCC2Exson 23和XRCC1密码子26304的遗传多态性可能与SCCHN的遗传易感性有关。采用聚合酶链式反应-限制性片段长度多态性方法对这些基因的多态性进行分析。XPD/ERCC2基因外显子23多态结果如下：对照组：野生型27例(93.1%)，杂合型2例(6.9%)，突变型0例(0%)，喉癌患者：野生型54例(91.5%)，杂合型5例(8.5%)，突变型0例(0%)。XRCC1基因26304位密码子多态性结果如下：对照：野生家族型25例(86.2%)，杂合型4例(13.8%)，突变型0例(0%)，患者：野生家族型52例(88.1%)，杂合型6例(10.2%)，突变型1例(1.7%)。XPD/ERCC2、XRCC1基因多态性与喉癌易感性无关。我们得出的结论是，这些多态并不是烟草相关癌症易感性的指标。

Genetic differences in the ability to metabolize carcinogens, repair DNA damage and induce apoptosis may contribute to the variation in cancer risk in population at large. The capacity of DNA repair genes may a play important role in carcinogenesis with Tabacco- related cancer. We hypothesize that inherited polymorphisms of XPD/ERCC2 exson 23 and XRCC1 codon 26304 may contribute to genetic susceptibility to SCCHN. These polymorphisms were investigated with PCR-RFLP method. The result of XPD/ERCC2 exson 23 polymorphisms are below, control : wild home type 27 cases (93.1%), hetero type 2 cases (6.9%), mutant type 0 case (0%), patient (laryngeal carcinoma ; SCC) : wild home type 54 cases (91.5%), hetero type 5 cases (8.5%), mutant type 0 case (0%). The result of XRCC1 codon 26304 polymorphisms are below, control : wild home type 25 cases (86.2%), hetero type 4 cases (13.8%), mutant type 0 case (0%), patient : wild home type 52 cases (88.1%), hetero type 6 cases (10.2%), mutant type 1 case (1.7%). There were no signicicant between XPD/ERCC2, XRCC1 polymorphisms and susceptibility to laryngeal carcinoma. We concluded that these polymorphisms are not indicator of susceptibility to Tabacco- related cancer.