RESEARCH ON APOPTOTIC CELL DEATH IN THE RAT HINDLIMB ALLOTRANSPLANTATION MODEL OF ACUTE REJECTION

进行了大鼠下肢异体移植（fisher 344 to lewis）和等体移植（lewis to lewis）。采用定量western blot和免疫组织化学方法对小鼠进行时间谱分析。同时，我们采用隧道法检测移植物中凋亡细胞的死亡情况。再融合后立即（第0天）和术后第1、3、7、12天（定量western blot分析n = 5，免疫组织化学分析n = 5）进行分析。与同种异体移植物相比，同种异体移植物中dna的表达水平均匀且显著增加（p < 0.001）。异体豆荚1上的角化细胞、毛囊细胞和真皮下血管丛内皮细胞对dna的免疫反应性增强。同时，我们检测到在相同类型的免疫组化阳性细胞中凋亡细胞死亡。这些结果提示凋亡细胞死亡可能参与mhc相容性小错配同种异体移植排斥反应的过程。因此，调节细胞凋亡的机制可能是改善mhc兼容的小错配同种异体移植物结果的重要治疗选择。

ALLOTRANSPLANTATION (FISHER 344 TO LEWIS) AND ISOTRANSPLANTATION (LEWIS TO LEWIS) OF THE RAT LOWER EXTREMITIES WERE PERFORMED. TEMPORAL PROFILES OF DNASEY WERE CONDUCTED BY QUANTITATIVE WESTERN BLOT ANALYSIS AND IMMUNOHISTOCHEMISTRY. ALSO, WE PERFORMED TUNEL TO DETECT APOPTOTIC CELL DEATH IN THE GRAFTS. ANALYSES WERE PERFORMED IMMEDIATELY AFTER REPER-FUSION (DAY 0), AND ON POSTOPERATIVE DAYS (PODS) 1, 3, 7,12 (N = 5 FOR QUANTITATIVE WESTER BLOT ANALYSIS AND N = 5 FOR IMMUNOHISTOCHEMISTRY).THE EXPRESSION LEVELS OF DNASEY WERE UNIFORMLY AND SIGNIFICANTLY AUGMENTED UNTIL POD 7 IN THE ALLOGRAFTS, COMPARED WITH ISOGRAFTS (P< 0.001). IMMUNOREACTIVITIES TO DNASEY IN KERATINOCYTES, HAIR FOLLICULAR CELLS AND ENDTHELIAL CELLS OF SUBDERMAL VASCULAR PLEXUS WERE ENHANCED IN THE ALLOGRAFTS ON POD 1. ALSO, WE DETECTED APOPTOTIC CELL DEATH IN THE SAME TYPES OF CELLS POSITIVE TO DNASEY IMMUNOHISTOCHEMISTRY.THESE RESULTS SUGGEST THE POSSIBLE INVOLVEMENT OF APOPTOTIC CELL DEATH IN THE PROCESS OF MHC-COMPATIBLE MINOR-MISMATCH ALLOGRAFT REJECTION. HENCE, MODULATION OF MECHANISMS OF APOPTOSIS MIGHT BE AN IMPORTANT THERAPEUTIC OPTION FOR IMPROVING THE OUTCOME OF MHC-COMPATIBLE MINOR-MISMATCH ALLOGRAFTS.